A5101823941- Adipose Tissue and Metabolism
- Iron Metabolism and Disorders
- Genetic and phenotypic traits in livestock
- Apelin-related biomedical research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Gut microbiota and health
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Nuclear Receptors and Signaling
- RNA modifications and cancer
- Reproductive Physiology in Livestock
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and biosynthesis
- Genetic Mapping and Diversity in Plants and Animals
- Helicobacter pylori-related gastroenterology studies
- Autophagy in Disease and Therapy
- Folate and B Vitamins Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- NF-κB Signaling Pathways
- Wnt/β-catenin signaling in development and cancer
- Bone and Dental Protein Studies
Guangxi Buffalo Research Institute
2017-2025
Chinese Academy of Agricultural Sciences
2019-2025
Shenzhen Institutes of Advanced Technology
2025
Chinese Academy of Sciences
2023-2025
Qinghai University
2024
Hunan University of Traditional Chinese Medicine
2024
Aptose Biosciences (United States)
2023
Institut Pasteur of Shanghai
2023
University of Chinese Academy of Sciences
2023
University of Michigan
2012-2022
Abstract Microfold cells (M-cells) are specialized of the intestine that sample luminal microbiota and dietary antigens to educate immune intestinal lymphoid follicles. The function M-cells in systemic inflammatory responses still unclear. Here we show epithelial non-canonical NFkB signaling mediated by NFkB-inducing kinase (NIK) is highly active follicles, required for M-cell maintenance. Intestinal NIK modulates differentiation elicits both local IL-17A IgA production. Importantly, mouse...
aa: amino acid; ATF6: activating transcription factor 6; ATG5: autophagy related 5; CCPG1: cell cycle progression 1; CFTR: CF transmembrane conductance regulator; cGAMP: cyclic GMP-AMP; CGAS: GMP-AMP synthase; CHX: cycloheximide; Co-IP: co-immunoprecipitation; CQ: chloroquine; EIF2A/eIF2α: eukaryotic translation initiation 2A; EIF2AK3/PERK: 2 alpha kinase 3; ER: endoplasmic reticulum; ERN1/IRE1: reticulum to nucleus signaling GFP: green fluorescent protein; HSPA5/GRP78: heat shock protein...
The mammary gland is the production organ in mammals that of great importance for milk and quality. However, characterization buffalo transcriptome identification valuable candidate genes affect limited. Here, we performed differential expressed (DEGs) analysis tissue on day 7, 50, 140, 280 after calving conducted gene-based genome-wide association studies (GWAS) yield 935 Mediterranean buffaloes. We then employed weighted gene co-expression network (WGCNA) to identify specific modules hub...
Abstract Bispecific antibodies (BsAbs) represent an emerging class of immunotherapy, but inefficiency in the current discovery has limited their broad clinical availability. Here we report a high throughput, agnostic, single-cell-based functional screening pipeline, comprising molecular and cell engineering for efficient generation BsAb library cells, followed by interrogation at single-cell level to identify sort positive clones downstream sequence identification functionality...
// Tao Wei 1, *, 4 , Li-Na Zhang 2, * Yi Lv 1 Xiao-Ya Ma 2 Lei Zhi 3 Chang Liu Feng Xu-Feng Department of Hepatobiliary Surgery and Institute Advanced Surgical Technology Engineering, the 1st Affiliated Hospital Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi Province, 710061, China Pharmacy, 2nd 710004, General Surgery, Ningxia Yinchuan, Hui Autonomous Region, 750004, Pancreatic The Second Hospital, Zhejiang University School Medicine, Hangzhou, 310009, These authors contributed...
Colorectal cancer (CRC) is the third-leading cause of mortality in United States and other industrialized countries. A hypoxic microenvironment a hallmark for solid tumors. The hypoxia-induced signal transduction transcriptionally mediated by hypoxia-inducible factor (HIF). Three major HIF isoforms, HIF-1α, HIF-2α, HIF-3α, are present intestine. Our previous work demonstrates that HIF-2α essential CRC growth progression. However, mechanisms mediating cell proliferation after hypoxia or...
Knowledge of RNA molecules regulating testicular development and spermatogenesis in bulls is essential for elite bull selection an ideal breeding program. Herein, we performed direct sequencing (DRS) to explore the functional characterization produced testicles 9 healthy Simmental at three stages (prepuberty, puberty, postpuberty). We identified 5,043 differentially expressed genes associated with weight. These exhibited more alternative splicing sexual maturity, particularly 3' (A3) 5' (A5)...
Apelin (APLN), as a ligand for APJ (an orphan G-protein-coupled receptor), is an adipokine with pleiotropic effects in many physiological processes of the body. It has important role control reproduction particularly females (mainly ovarian function). This study was carried out to investigate mRNA and protein amounts APLN/APJ granulose cells (GCs) follicles small (SF), medium (MF), large (LF) sizes buffalo (Bubalus bubalis) effect IGF1 follicle-stimulating hormone (FSH) on expression levels...
Calf diarrhea is of great concern to the global dairy industry as it results in significant economic losses due lower conception rates, reduced milk production, and early culling. Although there evidence an association between altered gut microbiota diarrhea, remarkably little known about microbial metabolic mechanisms underlying link dysbiosis occurrence calf diarrhea. Here, we used fecal metagenomic metabolomic analyses demonstrate that microbiota-driven disorders purine or arachidonic...
Background Laryngeal cancer (LC) is characterized by high mortality and remains challenging in prognostic evaluation treatment benefits. Ubiquitin-related genes (UbRGs) are widely involved initiation progression, but their potential value LC unknown. Methods RNA-seq clinical data of were obtained from TCGA GEO. UbRGs that independently influenced the overall survival (OS) patients screened with differential expression, COX LASSO regression analyses. A signature was then established assessed...
Background Iron dysregulation is a potential contributor to the pathology of obesity-related metabolic complications. KK/HIJ (KK) mice, polygenic obese mouse model, have elevated serum iron levels. A subset KK male mice display bronzing epididymal adipose tissue (eAT) associated with >100-fold (p<0.001) higher concentration. Methods To further phenotype and characterize overload, 27 were evaluated. 14 had eAT 13 normal appearing eAT. Fasting tissues collected for content, qPCR, histology...
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Endometritis is a uterine infection caused by bacterial pathogens and has detrimental effects on productive reproductive performance in dairy cows. A large number of studies have demonstrated the association gut microbiota with infectious diseases. However, role cows endometritis still poorly understood. In present study, we characterized fecal microbial populations suffering from metritis (n = 10) healthy 9) using 16 S rRNA gene sequencing. Results revealed an increased abundance Firmicutes...
Hypoxia-inducible factor 2α (HIF2α) directly regulates a battery of genes essential for intestinal iron absorption. Interestingly, deficiency and overload disorders do not result in increased expression glycolytic or angiogenic HIF2α target genes. Similarly, inflammatory tumor foci can induce distinct subset vivo. These observations indicate that different stimuli activate subsets via mechanisms remain unclear. Here, we conducted high-throughput siRNA-based screen to identify regulate...
The prevalence of iron deficiency tends to be higher in athletic populations, especially among endurancetrained females. Recent studies have provided evidence that the iron-regulating hormone hepcidin is transiently increased with acute exercise and suggest this may contribute anemia athletes. purpose study was determine whether resting serum significantly elevated highly trained female distance runners compared a low control group. Due importance monocyte process recycling, expression also...
Collagens, as extracellular matrix proteins, support cells for structural integrity and contribute to mammary basic structure development. This study aims perform the genomic identification, evolution, expression analyses of collagen gene family in water buffalo (Bubalus bubalis) during lactation. A total 128 protein sequences were deduced from 45 genes identified silico genome, which classified into six groups based on their phylogenetic relationships, conserved motifs, analyses. The...
Testis is the primary organ of male reproductive tract in mammals that plays a substantial role spermatogenesis. Improvement our knowledge regarding molecular mechanisms testicular development and spermatogenesis will be reflected producing spermatozoa superior fertility. Evidence showed N6-Methyladenosine (m6A) dynamic post-transcription gene expression regulation strongly associated with production traits. However, m6A bovine testis has not been investigated yet. In this study, we...
Identification of selection signature is important for a better understanding genetic mechanisms that affect phenotypic differentiation in livestock. However, the genome-wide responses have not been investigated production traits Chinese crossbred buffaloes. In this study, an SNP data set 133 buffaloes (Chinese buffalo, n = 45; local swamp 88) was collected from Dryad Digital Repository database (https://datadryad.org/stash/). Population genetics analysis showed these were divided into...
Cytochrome P450 aromatase 19A1 (CYP19A1) is a critical enzyme in estrogen synthesis. However, the effect of CYP19A1 on cell growth and hormone secretion buffalo follicular granulosa cells (BFGCs) poorly understood. The objective this study was to assess role proliferation BFGCs by knocking down mRNA expression. expression level gene knocked using siCYP19A1-296 fragment with best interference efficiency 72.63%, as affirmed real-time quantitative PCR (qPCR) morphology analysis. knockdown...