Neonatal calves’ diarrhea, which can be severe enough to cause death, has a significant impact on the global cattle industry. In this study, alfalfa polysaccharides and seaweed were found significantly improve diarrhea condition in neonatal calves. To explore underlying mechanisms, further microbiomic metabolomic analyses conducted. This study investigated of growth performance, serum metabolites, gut microbiota, metabolomics Holstein A total 24 newborn calves randomly assigned three groups,...

With a rich breeding history, Nanyang cattle (NY cattle) have undergone extensive natural and artificial selection, resulting in distinctive traits such as high fertility, excellent meat quality, disease resistance. This makes them an ideal model for studying the mechanisms of environmental adaptability. To assess population structure genetic diversity NY cattle, we performed whole-genome resequencing on 30 individuals. These data were then compared with published from 432 globally. The...

Heat stress influences rumen fermentative processes with effects on the physiology and production of dairy cows. However, underlying relationship between microbiota its associated metabolism heat tolerance in cows have not been extensively described yet. Therefore, main objective this study was to investigate differential resistance Holstein using bacterial metabolome analyses. We performed both principal component analysis membership function select seven heat-tolerant (HT) heat-sensitive...

Knowledge of RNA molecules regulating testicular development and spermatogenesis in bulls is essential for elite bull selection an ideal breeding program. Herein, we performed direct sequencing (DRS) to explore the functional characterization produced testicles 9 healthy Simmental at three stages (prepuberty, puberty, postpuberty). We identified 5,043 differentially expressed genes associated with weight. These exhibited more alternative splicing sexual maturity, particularly 3' (A3) 5' (A5)...

Abstract Supplementation with selenium is common for dairy cows, but the importance of source not clear. This study aimed to compare nano-selenium (Nano-Se) and sodium selenite supplements cows on lactation performance, milk Se levels selenoprotein (Sel) gene expression. Twelve multiparous Holstein were randomly divided into two groups: a control group fed basal diet plus 0.30 mg Se/kg DM as or Nano-Se 30 days. Dry matter intake, yield composition affected by dietary ( P > 0.05); however,...

Exposure to the stress (HS) negatively affects physiology, performance, reproduction and welfare of buffalo. However, mechanisms by which HS rumen bacteria its associated metabolism in buffalo are not well known yet. This study aimed gain insight into adaption complexity metabolome six during using 16S rDNA gas chromatography metabolomics analyses. increased respiratory rate (p < 0.05) skin temperature 0.01), it decreased content acetic acid butyric rumen. Omics sequencing revealed that...

Maize silage has a significant environmental impact on livestock due to its high requirement for fertilizer and water. Mulberry the potential replace much of large amount maize grown in China, but feeding value conserved form needs be evaluated. We fed Hu lambs diets with 20–60% replaced by mulberry silage, adjusting soybean meal content when increasing inclusion rate an attempt balance crude protein diets. had higher lower acidic neutral detergent fiber contents compared silage. Replacing...

Reportedly, the number of κ-casein (κ-CN) B alleles increases proportion κ-CN to total protein and content. This phenomenon is caused by single-nucleotide polymorphisms (SNPs) in promoter region CSN3, which encodes variant. Therefore, a series 5'-deleted plasmids were constructed define core CSN3. The activity was analyzed comparing luciferase among recombinant vectors with truncated promoters. No mutation occurred (5'-ctatcgtcagatctttcctttctgtcatcttcctattggtg-3') CSN3 40 cows. A 2092 bp...

This study aimed to investigate the flavor formation and meat quality differences among different beef cuts in Chinese Wagyu cattle. The metabolites gene expression profiles of chuck, neck, rump, tenderloin, longissimus lumborum were analyzed. results revealed that a total 240 volatile organic compounds 779 lipid molecules detected cuts, with hydrocarbons (accounting for 29.71%) triglycerides (representing 41.21%) emerging as most prominent compounds, respectively. sensory-directed analysis...

Objectives This cross-sectional study aimed to assess parents’ knowledge, attitude and practice (KAP) towards myopia amidst increased children’s online course participation during the COVID-19 pandemic, potentially impacting visual health. Design The adopted a design analyse parent KAP regarding myopia. Setting participants Conducted from 19 August 2022 October 2022, in Jinan High-tech District, included 3261 participants, comprising 800 males (24.5%). Interventions A self-administered...

The study aimed to compare the growth performance, rumen fermentation and bacterial community of fattening Hu sheep fed either total mixed ration (TMR) or pelleted (PTMR), assess feeding efficiency. In a 58-day experiment, forty-eight were randomly assigned two groups (TMR PTMR), with six pens per group four pen. Body weight feed intake measured throughout experiment performance. On final day fluid was collected from using collector hours post-feeding. in PTMR exhibited significantly higher...

Background: Xianan cattle, the first cross-bred beef cattle developed in China, are recognized for their rapid growth, tolerance to rough feed, and high meat yield. These characteristics make them a valuable model studies aimed at improving production traits. Methods: In this study, two complementary gene mapping strategies, selection signature analysis association analysis, were employed identify candidate genes associated with body weight. The analyses utilized resequencing data comprising...

Melatonin (MT) influences lipid metabolism in animals; however, the mechanistic effect of melatonin on liver fat and abdominal adipose deposition requires further clarity. In order to study effects metabolism, hepatic animals, twenty Sprague-Dawley (SD) rats 6 weeks age with similar bodyweight were randomly divided into two groups: control (CTL) MT-treated (10 mg/kg/day). During a 60-day experiment, food intake measured daily weekly respectively. At end treatment, blood samples collected...

Metabolism in most organisms can show variations between the day and night. These may also affect composition of products derived from livestock. The aim present study was to investigate difference milk night dairy cows. Ten multiparous Holstein cows (milk yield = 25.2 ± 5.00 kg/d) were randomly selected during mid lactation. Milk samples collected at 0500 h ("night milk") 1500 ("day analyzed determine their composition. Mid-infrared spectroscopy used analyze macronutrient content milk....

Abstract α-Lipoic acid (α-LA) was usually applied to improve the ability of removing reactive oxygen species host. The affection α-LA on ruminants mainly focused variation serum antioxidant and immune indexes, but research tissues or organs remained limited. aim this study explore effects dietary supplementation with different levels growth performance, status, indexes in sheep. One hundred Duhu F1 hybrid (Dupo♀ × Hu sheep♂) sheep aged 2 3 mo similar body weight (27.49 ± 2.10 kg) were...

This study aimed to assess the effects of feeding with different forage sources and starter concentrations on growth performance, nutrient digestibility, ruminal fermentation, microbial community in weaned Holstein calves. A total 54 calves (body weight (BW) = 77.50 ± 5.07 kg; age 70 2.54 days) were assigned 1 3 treatment groups ( n 18/group) that offered diets forages: (1) peanut vine (PV), (2) oat hay (OH), or (3) an alfalfa + combination (alfalfa hay:oat =1:1, AO). Starter intakes...

Streptococcus pluranimalium is a new member of the genus isolated from multiple different animal hosts. It has been identified as pathogen associated with subclinical mastitis, valvular endocarditis and septicaemia in animals. Moreover, this bacterium emerged for human infective brain abscess. However, patho-biological properties S. remain virtually unknown. The aim study was to determine complete genome sequence strain TH11417 cattle characterize its antimicrobial resistance, virulence,...

Introduction The panicle fertilization strategy for japonica and indica rice under wheat straw return (SR) has not been updated, especially on the elaboration of their impacts spikelet differentiation degeneration. This study aimed to verify hypothesis that SR increases number by reducing degeneration explore possibility simplifying fertilization. Methods In three consecutive years, four varieties were field-grown in Yangzhou, Jiangsu Province, China. Six rates split treatments applied no...

This study systematically investigated the effects of tea polyphenols on methane (CH4) production and rumen epithelial cell transport capability in cattle using both vitro animal experiments, employing multi-omics techniques. The results demonstrated that, compared to control group, significantly reduced CH4 acetate/propionate ratio (p < 0.05). Tea by inhibiting relative abundance unclassified_d_Archaea methanogens protozoa Pseudoentodinium g__Balantioides. experiments showed that...