A5029155964- Digestive system and related health
- Cancer Cells and Metastasis
- Wnt/β-catenin signaling in development and cancer
- Vitamin D Research Studies
- MicroRNA in disease regulation
- Genetic factors in colorectal cancer
- RNA modifications and cancer
- Ion Transport and Channel Regulation
- Helicobacter pylori-related gastroenterology studies
- Hippo pathway signaling and YAP/TAZ
- Cancer-related gene regulation
- Colorectal Cancer Surgical Treatments
- Congenital gastrointestinal and neural anomalies
- Extracellular vesicles in disease
- Colorectal Cancer Treatments and Studies
- Caveolin-1 and cellular processes
- Clinical Nutrition and Gastroenterology
- Congenital Diaphragmatic Hernia Studies
- Parathyroid Disorders and Treatments
- Mycobacterium research and diagnosis
- Skin and Cellular Biology Research
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- Cellular transport and secretion
Rutgers, The State University of New Jersey
2017-2025
Environmental and Occupational Health Sciences Institute
2022-2025
National Institute of Environmental Health Sciences
2025
Instituto Nacional de Salud
2020-2022
Rutgers Cancer Institute of New Jersey
2017
University of Notre Dame
2011-2013
Oncogenic mutations in BRAF are believed to initiate serrated colorectal cancers; however, the mechanisms of BRAF-driven colon cancer unclear. We find that oncogenic paradoxically suppresses stem cell renewal and instead promotes differentiation. Correspondingly, tumor formation is inefficient mouse models cancer. By reducing levels differentiation via genetic manipulation either two distinct differentiation-promoting factors (Smad4 or Cdx2), activity restored BRAFV600E intestines, capacity...
Epithelial organoids derived from intestinal tissue, called enteroids, recapitulate many aspects of the organ in vitro and can be used for biological discovery, personalized medicine, drug development. Here, we interrogated cell signaling environment within developing human intestine to identify niche cues that may important epithelial development homeostasis. We identified an EGF family member, EPIREGULIN (EREG), which is robustly expressed crypt. Enteroids generated grown standard culture...
Wnt signaling has an essential role in embryonic development as well stem/progenitor cell renewal, and its aberrant activation is implicated many diseases, including several cancers. β-Catenin a critical component of Wnt-mediated transcriptional activation. Here we show that ARF6 during canonical promotes the intracellular accumulation β-catenin via mechanism involves endocytosis growth factor receptors robust extracellular signal-regulated kinase (ERK). ERK casein 2-mediated phosphorylation...
Stromal fibroblasts regulate critical signaling gradients along the intestinal crypt-villus axis 1 and provide a niche that supports adjacent epithelial stem cells. Here we report Pdgfra -expressing secrete ligands promote regenerative-like state in mucosa during early WNT-mediated tumorigenesis. Using mouse model of WNT-driven oncogenesis single-cell RNA sequencing (RNA-seq) mesenchyme cell populations, revealed dynamic reprogramming + facilitates tissue transformation. Functional assays...
SUMMARY Colorectal cancer (CRC) is a leading cause of deaths, predominantly initiated by genetic inactivation the APC tumor suppressor. Current dogma holds that promotes tumorigenesis via hyperactivation WNT pathway transcriptional effector, β-CATENIN. Although β-CATENIN activation required for initiation, activating mutations in are infrequent. Here, we ask what underlies selective pressure comparing oncogenic effects loss to hyperactivation. We find activates β-CATENIN-independent fetal...
Although vitamin D is critical for the function of intestine, most studies have focused on duodenum. We show that transgenic expression receptor (VDR) only in distal intestine VDR null mice (KO/TG mice) results normalization serum calcium and rescue rickets. it had been suggested transport involves a paracellular process, we found 1,25-dihydroxyvitamin
The adult gut epithelium has a remarkable ability to recover from damage. To achieve cellular therapies aimed at restoring and/or replacing defective gastrointestinal tissue, it is important understand the natural mechanisms of tissue regeneration. We employed combination high throughput sequencing approaches, mouse genetic models, and murine human organoid identified role for TGFB signaling during intestinal regeneration following injury. At 2 days irradiation (IR)-induced damage crypts,...
Tuberculosis (TB) is one of the most fatal infectious diseases, caused by aerobic bacteria Mycobacterium tuberculosis . It estimated that one-third world’s population infected with latent (LTB) version this disease, only 5-10% individuals developing its active (ATB) form. Pulmonary adenocarcinoma (PA) common and diverse form primary lung carcinoma. The simultaneous or sequential occurrence TB cancer in patients has been widely reported known to be an issue for diagnosis surgical treatment....
SLC26A3 [downregulated in adenoma (DRA)] plays a key role mammalian intestinal NaCl absorption, that it mediates apical membrane Cl − /[Formula: see text] exchange. DRA function and expression are significantly decreased diarrhea associated with inflammatory bowel disease. is also considered to be marker of cellular differentiation predominantly expressed differentiated epithelial cells. Caudal-type homeobox protein-2 (CDX2) known regulate genes involved proliferation. Reduced both CDX2...
1,25-dihydroxyvitamin D (VD) regulates intestinal calcium absorption in the small intestine (SI) and also reduces risk of colonic inflammation cancer. However, compartment-specific target genes VD signaling are unknown. Here, we examined action across three functional compartments using RNA-seq to measure VD-induced changes gene expression Chromatin Immunoprecipitation with next generation sequencing vitamin receptor (VDR) genomic binding. We found that regulated 55 shared transcripts SI...
ABSTRACT Although Wnt signaling is clearly important for the intestinal epithelial homeostasis, relevance of various sources ligands themselves remains incompletely understood. Blocking release in distinct stromal cell types suggests obligatory functions several and yields different observations. The physiological contribution to tissue homeostasis unclear. We show here that blocking Wnts affects colonic Reg4+ differentiation impairs regeneration after injury mice. Single-cell RNA analysis...
To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) active TB compared to healthy controls.We used 28 (9 TB, 10 9 con trols) for analysis gene by RT-qPCR Primers TaqMan probes. The was calculated Livak method using a normalizing (RNU-48).Overex pression found people tuberculosis, controls (0.63 vs. 0.01; p value = 0.032).The could be considered biomarker differentiate disease. Studies larger sample sizes...
Background SLC26A3 (Down Regulated in Adenoma, DRA) plays a key role mammalian intestinal NaCl absorption via mediating apical membrane Cl − /HCO 3 exchange. DRA function and expression is significantly decreased diarrheal diseases associated with infections inflammatory bowel (IBD). Since, now being touted as an important therapeutic target for infectious IBD diarrhea, it critical to understand the detailed mechanisms of regulation at molecular cellular level health disease. Earlier studies...
Vitamin D receptor (VDR) is a transcription factor that mediates calcium absorption by intestinal epithelial cells. Although ca-nonically thought to occur only in the small intestine, recent studies have shown VDR activity co-lon alone sufficient prevent deficiency mice. Here, we further investigate colon. We assess VDR-DNA binding mouse duodenal crypt, villi, and colonic cells using Chromatin Immunoprecipitation se-quencing (ChIP-seq). find most VDR-respon-sive elements are common all...