A5077420454- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Genetic Associations and Epidemiology
- Forensic and Genetic Research
- Pharmacogenetics and Drug Metabolism
- Genomics and Rare Diseases
- Race, Genetics, and Society
- Genomic variations and chromosomal abnormalities
- Nutrition, Genetics, and Disease
- Drug-Induced Hepatotoxicity and Protection
- RNA and protein synthesis mechanisms
- Occupational Health and Safety in Workplaces
- interferon and immune responses
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Mosquito-borne diseases and control
- Genomics and Phylogenetic Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- 3D Printing in Biomedical Research
- Zoonotic diseases and public health
- Infectious Diseases and Tuberculosis
- Hydraulic and Pneumatic Systems
- Diagnosis and treatment of tuberculosis
- RNA modifications and cancer
- Viral Infections and Vectors
Instituto Nacional de Salud
2016-2025
Universidad de Huánuco
2019-2025
Universidad Norbert Wiener
2022-2023
Universidad Científica del Sur
2018-2022
Ministerio de Educación del Perú
2021
Universidad de Ingeniería y Tecnología
2020-2021
National Center for Genetic Engineering and Biotechnology
2010-2020
Instituto de Salud Pública de Chile
2020
University of Chile
2020
Instituto Nacional de Salud del Niño
2018
Abstract As whole-genome sequencing (WGS) becomes the gold standard tool for studying population genomics and medical applications, data on diverse non-European admixed individuals are still scarce. Here, we present a high-coverage WGS dataset of 1,171 highly elderly Brazilians from census-based cohort, providing over 76 million variants, which ~2 absent large public databases. enables identification ~2,000 previously undescribed mobile element insertions without previous description, nearly...
Significance Through the Peruvian Genome Project we generate and analyze genomes of 280 individuals where majority have >90% Native American ancestry explore questions at interface evolutionary genetics, history, anthropology, medicine. This is most extensive sampling high-coverage mestizo whole to date. We estimate an initial peopling Peru was rapid began by 12,000 y ago. In addition, populations exhibit admixture between groups prior their Spanish likely influenced Inca Empire conquest....
Significance Native Americans are neglected in human genetics studies, despite recent interest the study of ancient DNA their ancestors. Our findings on Andean and Amazonian populations exemplify how current pattern genetic diversity is influenced by interaction history environment. In present case, this 1) altitudinal climatic differences among northern, lower, fertile Andes versus southern, higher, arid 2) sharp between highlands Amazon lowlands, where natural selection other evolutionary...
The Transatlantic Slave Trade transported more than 9 million Africans to the Americas between early 16th and mid-19th centuries. We performed a genome-wide analysis using 6,267 individuals from 25 populations infer how different African groups contributed North-, South-American, Caribbean populations, in context of geographic geopolitical factors, compared genetic data with demographic history records Trade. observed that West-Central Africa Western Africa-associated ancestry clusters are...
The metabolic conversion of dietary omega-3 and omega-6 18 carbon (18C) to long chain (>20 carbon) polyunsaturated fatty acids (LC-PUFAs) is vital for human life. rate-limiting steps this process are catalyzed by acid desaturase (FADS) 1 2. Therefore, understanding the evolutionary history FADS genes essential our hominin evolution. have two haplogroups, ancestral derived, with derived haplogroup being associated more efficient LC-PUFA biosynthesis than haplogroup. In addition, there a...
Background Early detection and diagnosis of tuberculosis remain major global priorities for control. Few studies have used a qualitative approach to investigate the social determinants contributing diagnostic delay none compared data collected from individual, community, health-system levels. We aimed characterize that contribute among persons diagnosed with living in resource-constrained settings. Methods/Principle findings Data were public health facilities high incidence 19 districts...
<title>Abstract</title> In Peru, 33 113 individuals were diagnosed with tuberculosis (TB) in 2023. While TB treatments are generally effective, 3.4–13% of cases associated significant adverse drug reactions, drug-induced liver injury (DILI) being the most prevalent. Limited data exist on genetic risk factors for DILI Latin America; even less is known about these native Peruvian populations. This study aimed to determine prevalence drug-metabolizing genotypes A cross-sectional analysis was...
The ESR1 gene is relevant in breast cancer treatments the pharmacogenetics context. However, Native, African, and mixed populations are known to be underrepresented genomic studies. This particularly important given that difference variants' frequencies among different can lead population‐specific clinical implications. Therefore, this study aims infer subcontinental ancestry allele of variants 2,427 individuals from 26 worldwide 1000 Genomes Project 125 Natives Peru, whose genomes have not...
Abstract Latin American populations exhibit high genetic and phenotypic diversity shaped by complex admixture histories, yet remain underrepresented in genomic research. Here, we analyze genome-wide data from 432 urban individuals across 13 regions of Peru, including 346 newly genotyped the Peruvian Genome Project. We revealed fine-scale population structure demographic patterns both ancient recent events. Indigenous ancestries trace back to north-south interactions consisted with...
Objective Systemic lupus erythematosus (SLE) results in worse clinical outcomes among individuals of Amerindian descent. The genetic basis for this is uncertain, and there a significant lack research focused on ancestry populations. This study aims to compare the frequencies SLE risk variants polygenic scores between Indigenous Peruvians global populations with diverse ancestral backgrounds. Methods We studied 670 from Peruvian Genome Project, 2,068 1000 Genomes Project Phase 3 release, 47...
M. tuberculosis (MTB) species-specific antigenic determinants of the human T cell response are important for immunodiagnosis and vaccination. As hypoxia is a stimulus in chronic infection, we analyzed transcriptional profiles MTB subject to 168 hours test hypothesis that upregulation by might result gene products being recognized as antigens. We identified two region difference (RD) 11 (Rv2658C Rv2659c), one RD2 (Rv1986) absent from commonly used BCG strains. In infected persons, IL-2...
We analyzed whole genome-based transcriptional profiles of Mycobacterium tuberculosis subjected to prolonged hypoxia guide the discovery novel potential Ags, by a combined bioinformatic and empirical approach. fold induction 100 most highly induced genes at 7 d hypoxia, as well transcript abundance, peptide-binding prediction (ProPred) adjusted for population-specific MHC class II allele frequency, literature search. Twenty-six candidate were selected this approach evaluated empirically...
The emergence of multidrug-resistant strains is a major health problem especially for countries with high TB incidence such as Peru. In this study, we evaluated High Resolution Melting (HRM) assay in Peruvian isolates the detection mutations within rpoB, katG genes and promoter region inhA to determine isoniazid rifampicin resistance Mycobacterium tuberculosis (Mtb). DNA samples extracted from total 167 clinical Mtb, 89 drug-sensitive 78 multidrug-resistant, were blindly analyzed by HRM...
The emergence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant (XDR-TB) strains is a major health problem for high Tuberculosis (TB) incidence countries. Therefore, it interest to identify antibiotic resistant bacteria by mismatch detection using DNA hybridization. We generated PCR products five genes (rpoB, inhA, katG, gyrA rrs) associated with drug resistance TB from MDR XDR Mycobacterium (MTB) samples. These were hybridized MTB H37Rv (pansusceptible laboratory...
In Peru, 32,970 people were diagnosed with tuberculosis (TB) in 2019. Although TB treatment is effective, 3.4%-13% associated significant adverse drug reactions (ADR), considering drug-induced liver injury (DILI) as the most prevalent. Among first-line anti-TB drugs, isoniazid (INH) primarily responsible for occurrence of DILI. INH metabolized by enzymes N-acetyltransferase-2 (NAT2) and Cytochrome P450 2E1 (CYP2E1). Based on previous studies, we hypothesized that interactions between slow...
Abstract In Peru, 29 292 people were diagnosed with tuberculosis in 2022. Although treatments are effective, 3.4%–13% associated significant adverse drug reactions, drug‐induced liver injury (DILI) considered the most predominant. Among first‐line antituberculosis drugs, isoniazid is main responsible for appearance of DILI. liver, (INH) metabolized by N‐acetyltransferase‐2 ( NAT2 ) and cytochrome P450 2E1 CYP2E1 ). Limited information exists on genetic risk factors presence DILI to drugs...
SUMMARY The process of inhabiting the Americas by ancestral native American populations involved many individuals settling in Peruvian Andes and Amazonian regions. Due to Latin countries representing less than 1% human genome data available public reference databases, evolution migration processes adapting this unique geography have not yet been fully explained. Genome Project is an initiative, started 2011, address underrepresentation genomic from South populations. This project has...
Tuberculosis (TB) is one of the most fatal infectious diseases, caused by aerobic bacteria Mycobacterium tuberculosis . It estimated that one-third world’s population infected with latent (LTB) version this disease, only 5-10% individuals developing its active (ATB) form. Pulmonary adenocarcinoma (PA) common and diverse form primary lung carcinoma. The simultaneous or sequential occurrence TB cancer in patients has been widely reported known to be an issue for diagnosis surgical treatment....