A5037770501- Cancer, Hypoxia, and Metabolism
- Synthesis and biological activity
- Multicomponent Synthesis of Heterocycles
- Digestive system and related health
- Diet and metabolism studies
- Glioma Diagnosis and Treatment
- Tryptophan and brain disorders
- RNA modifications and cancer
- Metal complexes synthesis and properties
- Gut microbiota and health
- CAR-T cell therapy research
- Nanoplatforms for cancer theranostics
- Pancreatic function and diabetes
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Ferrocene Chemistry and Applications
- Pharmacology and Obesity Treatment
- Quinazolinone synthesis and applications
- Adipose Tissue and Metabolism
- Corrosion Behavior and Inhibition
- Neuroblastoma Research and Treatments
- Bioactive Compounds and Antitumor Agents
- Virus-based gene therapy research
- ATP Synthase and ATPases Research
- Cancer, Lipids, and Metabolism
Rutgers, The State University of New Jersey
2022-2025
Environmental and Occupational Health Sciences Institute
2024
Rutgers Cancer Institute of New Jersey
2024
Rutgers Sexual and Reproductive Health and Rights
2024
Memorial Sloan Kettering Cancer Center
2018-2022
Skolkovo Institute of Science and Technology
2019
University of Hyderabad
2012-2014
Previous studies show that LDH-A knockdown reduces orthotopic 4T1 breast tumor lactate and delays growth the development of metastases in nude mice. Here, we report significant changes microenvironment (TME) a more robust anti-tumor response immune competent BALB/c murine cancer cells were transfected with shRNA plasmids directed against (KD) or scrambled control plasmid (NC). Cells also transduced dual luciferase-based reporter systems to monitor HIF-1 activity by bioluminescence imaging,...
A series of eight pyruvate-based aroylhydrazones was synthesised and characterised. The reaction the sodium salts with one equivalent copper(II) chloride allowed isolation neutral 1 : complexes in which hydrazones occupy three basal coordination sites a square pyramidal Cu(II)-centre, two solvent molecules completing sphere. Structural details were obtained through determination crystal structures representative Cu(II) complexes. evaluation antimycobacterial activity pryruvate showed that...
To enhance human prostate-specific membrane antigen (hPSMA)-specific chimeric receptor (CAR) T cell therapy in a hPSMA
The effects of the LDH-A depletion via shRNA knockdown on three murine glioma cell lines and corresponding intracranial (i.c.) tumors were studied compared to pharmacologic (GNE-R-140) inhibition LDH enzyme complex, scrambled control (NC) lines. genetic-shRNA drug-targeted tumor-cell metabolism, tumor growth, animal survival similar. KD GNE-R-140 unexpectedly increased aggressiveness GL261 gliomas, but not CT2A ALTS1C1 i.c. gliomas. Furthermore, bioenergetic profiles (ECAR OCR) NC cells...
Terminal differentiation requires massive restructuring of the transcriptome. During intestinal differentiation, expression patterns nearly 4,000 genes are altered as cells transition from progenitor in crypts to differentiated villi. We identify dynamic occupancy RNA polymerase II (Pol II) gene promoters primary driver transcriptomic shifts during vivo. Changes enhancer-promoter looping interactions accompany Pol and dependent upon HNF4, a pro-differentiation transcription factor. Using...
Three murine glioma cell lines (GL261, CT2A, and ALTS1C1) were modified to downregulate the expression of LDH-A gene using shRNA, compared shRNA scrambled control (NC) lines. Differences in LDH-B mRNA, protein enzymatic activity, as well their LDH isoenzyme profiles, observed six lines, confirmed successful KD. KD (knock-down) resulted metabolic changes cells with a reduction glycolysis (GlycoPER) an increase basal respiratory rate (mitoOCR). GL261 had more limited ATP production capacity...
Depression is a prevalent psychological condition with limited treatment options. While its etiology multifactorial, both chronic stress and changes in microbiome composition are associated disease pathology. Stress known to induce dysbiosis, defined here as change microbial pathological condition. This state of dysbiosis feedback on depressive symptoms. studies have demonstrated that targeted restoration the can alleviate depressive-like symptoms mice, translating these findings human...
Summary Obesity has become an epidemic, prompting advances in therapies targeting this condition. Estrogen-related receptor α (ESRRA), a transcription factor, plays pivotal roles energy metabolism across diverse tissues. Studies have demonstrated that loss of Esrra leads to fat malabsorption and resistance diet-induced obesity. However, the reliance these studies on germline mutants overlooks tissue-specific implications ESRRA Notably, exhibits high expression gastrointestinal (GI) tract...
Abstract Background: Lactate metabolism in tumors is now recognized as a major energy source and gluconeogenic precursor for many tumors, well shown to exhibit signaling properties. There less information on the role of LDH/lactate axis brain although lactate formation gliomas associated with poor survival. Methods: Three murine glioma cell lines (GL261, CT2A, ALTS1C1) were transduced knockdown (KD) expression LDH-A gene. The effects KD compared those control (NC) cells tumors. Results:...
SUMMARY Terminal differentiation requires a massive restructuring of the transcriptome. During intestinal differentiation, expression patterns nearly 4000 genes are altered as cells transition from progenitor in crypts to differentiated villi. We identified dynamic recruitment RNA Polymerase II (Pol II) gene promoters primary driver transcriptomic shifts during vivo . Changes enhancer-promoter looping interactions accompany Pol and dependent upon HNF4, pro-differentiation transcription...
Abstract Utilizing the Biginelli reaction, a series of dihydropyrimidines (IV) is synthesized and evaluated for in vitro anticancer (MCF‐7) antitubercular (MTB) potency.
Abstract Objective: Determine whether LDH-A knockdown enhances CAR T-cell tumor-targeting and treatment response.Background: It has been shown that T-cells are restricted from entering many human murine solid tumors, including both prostate tumors. CD3(+) Myc-CaP tumors localize around the periphery of tumor nodules (1). Human specific membrane antigen (hPSMA) targeted also hPSMA(+) this is only partially reversed by anti-PD1 This report describes benefit depletion a greater response...
Abstract Abnormal vessel formation plays a major role in tumor progression. Previously, we showed that lactate dehydrogenase A (LDH-A) knockdown (KD) reduces orthotopic 4T1 breast lactate, and has robust anti-tumor effect through changes the microenvironment (TME) [1, 2]. Tumors with LDH-A downregulation have lower VEGF-A secretion reduced of new blood vessels [2]. In addition, KD cells/tumors displayed HIF-1 signaling, as assessed by BLI using HRE-exGLuc reporter. Changes bioluminescence...
Abstract Depression is a prevalent psychological condition with limited treatment options. While its etiology multifactorial, both chronic stress and changes in the microbiome are associated disease pathology. In depression, known to induce dysbiosis, defined here as change microbial composition pathological condition. This state of dysbiosis then feedback on depressive symptoms. studies have demonstrated that targeted restoration can alleviate depressive-like symptoms mice, translating...