A5006257595- Advanced MRI Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- MRI in cancer diagnosis
- Medical Imaging Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Metabolomics and Mass Spectrometry Studies
- Radiomics and Machine Learning in Medical Imaging
- Angiogenesis and VEGF in Cancer
- Cancer Research and Treatments
- Spectroscopy and Chemometric Analyses
- Cancer, Lipids, and Metabolism
- Mitochondrial Function and Pathology
- Cancer Immunotherapy and Biomarkers
- Lanthanide and Transition Metal Complexes
- Advanced Fluorescence Microscopy Techniques
- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Cancer-related Molecular Pathways
- Immune cells in cancer
- Virus-based gene therapy research
- CAR-T cell therapy research
- Ubiquitin and proteasome pathways
- Cancer Genomics and Diagnostics
- bioluminescence and chemiluminescence research
- Electron Spin Resonance Studies
Memorial Sloan Kettering Cancer Center
2012-2024
The University of Texas MD Anderson Cancer Center
2023
Kettering University
2010-2022
Skolkovo Institute of Science and Technology
2019
Cornell University
2012
Johns Hopkins University
2000-2011
Johns Hopkins Medicine
2000-2010
Sidney Kimmel Comprehensive Cancer Center
2007
Resonance Research (United States)
2001
Cancer cells adapt their metabolism during tumorigenesis. We studied two isogenic breast cancer lines (highly metastatic 4T1; nonmetastatic 67NR) to identify differences in glucose and glutamine response metabolic environmental stress. Dynamic magnetic resonance spectroscopy of 13C-isotopomers showed that 4T1 have higher glycolytic tricarboxylic acid (TCA) cycle flux than 67NR readily switch between glycolysis oxidative phosphorylation (OXPHOS) different extracellular environments. OXPHOS...
In vivo knowledge of the spatial distribution viable, necrotic, and hypoxic areas can provide prognostic information about risk developing metastases regional radiation sensitivity may be used potentially for localized dose escalation in treatment. this study, multimodality magnetic resonance imaging (MRI) positron emission tomography (PET) using stereotactic fiduciary markers Dunning R3327AT prostate tumor were performed, focusing on relationship between dynamic contrast-enhanced (DCE) MRI...
Several studies have linked vascular density, identified in histologic sections, to “metastatic risk.” Functional information of the vasculature, not readily available from can be obtained with contrast-enhanced MRI exploit for therapy or metastasis prevention. Our aims were determine if human breast and prostate cancer xenograffs preselected differences invasive metastatic characteristics established correspondingly different volume permeability, quantified here noninvasive intravascular...
Although the mechanisms through which hypoxia influences several phenotypic characteristics such as angiogenesis, selection for resistance to apoptosis, radiation and chemotherapy, increased invasion metastasis are well characterized, relationship between tumor components of extracellular matrix (ECM) is relatively unexplored. The collagen I (Col1) fiber solid tumors major structural part ECM. Col1 density can increase initiation, progression, metastasis, with cancer cell occurringalong...
Abstract Tumor heterogeneity can be elucidated by mapping subregions of the lesion with differential imaging characteristics, called habitats. Dynamic Contrast Enhanced (DCE-)MRI depict tumor microenvironments identifying areas variable perfusion and vascular permeability, since individual habitats vary in rate magnitude contrast uptake washout. Of particular interest is hypoxia, characterized inadequate hyper-permeable vasculature. An automatic procedure for delineation from DCE-MRI was...
Tumor cure with conventional fractionated radiotherapy is 65%, dependent on tumor cell-autonomous gradual buildup of DNA double-strand break (DSB) misrepair. Here we report that single-dose (SDRT), a disruptive technique ablates more than 90% human cancers, operates distinct dual-target mechanism, linking acid sphingomyelinase-mediated (ASMase-mediated) microvascular perfusion defects to unrepair in cells confer cell lethality. ASMase-mediated microcirculatory vasoconstriction after SDRT...
A direct correlation exists between increased choline kinase (Chk) expression, and the resulting increase of phosphocholine levels, histological tumor grade. To better understand function Chk phospholipid metabolism in breast cancer we have stably overexpressed one two isoforms Chk-alpha known to be upregulated malignant cells, non-invasive MCF-7 human cells. Dynamic tracking cell invasion were studied with a magnetic resonance (MR) compatible perfusion assay. The MR based assay demonstrated...
In solid tumors, hypoxia contributes significantly to radiation and chemotherapy resistance poor outcomes. The "gold standard" pO(2) electrode measurements of in vivo are unsatisfactory because they invasive have limited spatial coverage. Here, we present an approach identify areas tumor using the signal versus time curves dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) data as a surrogate marker hypoxia. We apply unsupervised pattern recognition (PR) technique determine...
Significance Solid tumors adapt a glycolytic phenotype for their energetic requirements, leading to acidification of the extracellular environment. Targeting this global event is important gauge pace tumor growth and invasiveness, as well provide basis predicting disease response pH-dependent chemotherapies. To realize goal, noninvasive method necessary measure meet clinical needs. This study explores utility pH (low) insertion peptide, an acidosis-targeting PET-based imaging probe...
Hostile physiological environments such as hypoxia and acidic extracellular pH, which exist in solid tumors, may promote invasion metastasis through inflammatory responses formation of eicosanoids. Here, we have investigated the effects antiinflammatory agent indomethacin on metabolism human breast cancer cell line MDAMB-435 Dulbecco's Modified Eagles (DME)-based or Roswell Park Memorial Institute (RPMI)-based medium, using a magnetic resonance-compatible assay. Indomethacin treatment...
The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines different metastatic ability are investigated by fluorescence spectroscopy. (fibroblast), moderately (DU-145), advanced (PC-3) were each selectively excited at 300 nm, their emission spectra analyzed using principal component analysis to explore the differences relative contents tryptophan reduced nicotinamide adenine dinucleotide in these lines. results show that featured predominantly cells...
Cancer growth and proliferation rely on intracellular iron availability. We studied the effects of Deferiprone (DFP), a chelator iron, three prostate cancer cell lines: murine, metastatic TRAMP-C2; non-metastatic Myc-CaP; human, 22rv1. The DFP were evaluated at different cellular levels: culture migration; metabolism live cells (time-course multi-nuclear magnetic resonance spectroscopy perfusion studies, with 1-13 C-glucose, extracellular flux analysis); expression (Western blot) activity...
Triple negative breast cancer (TNBC) is a highly aggressive form of cancer. Detecting TNBC early crucial for improving disease prognosis and optimizing treatment. Unfortunately, conventional imaging techniques fall short in providing comprehensive differentiation subtypes due to their limited sensitivity inability capture subcellular details. In this study, we present multimodal platform that integrates heavy water (D 2 O)-probed stimulated Raman scattering (DO-SRS), two-photon fluorescence...
Triple-negative breast cancer (TNBC) is an aggressive subset of that more common in African-American and Hispanic women. Early detection followed by intensive treatment critical to improving poor survival rates. The current standard diagnose TNBC from histopathology biopsy samples invasive time-consuming. Imaging methods such as mammography magnetic resonance (MR) imaging, while covering the entire breast, lack spatial resolution specificity capture molecular features identify TNBC. Two...
To enhance human prostate-specific membrane antigen (hPSMA)-specific chimeric receptor (CAR) T cell therapy in a hPSMA
Abstract Choline metabolites in malignant human mammary epithelial cells (HMECs) are significantly altered compared to normal HMECs. 1 H NMR studies of cell extracts have shown that treatment HMECs with a nonsteroidal anti‐inflammatory agent, indomethacin, results distribution choline compounds more typical nonmalignant To follow the time course these changes, this study real‐time monitoring MDA‐MB‐231 was performed during indomethacin. The contribution changes intra‐ and extracellular pH...