A5100630790- Intraocular Surgery and Lenses
- Connexins and lens biology
- Ophthalmology and Visual Impairment Studies
- Corneal surgery and disorders
- Ocular Disorders and Treatments
- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- ATP Synthase and ATPases Research
- TGF-β signaling in diseases
- Renal and related cancers
- Mitochondrial Function and Pathology
- Ocular and Laser Science Research
- Genomics, phytochemicals, and oxidative stress
- Hedgehog Signaling Pathway Studies
- Connective Tissue Growth Factor Research
- melanin and skin pigmentation
- Solid State Laser Technologies
- Iron Metabolism and Disorders
- Ferroptosis and cancer prognosis
- Ocular Infections and Treatments
- Endoplasmic Reticulum Stress and Disease
- Retinoids in leukemia and cellular processes
- Developmental Biology and Gene Regulation
- Glutathione Transferases and Polymorphisms
- Cassava research and cyanide
China Medical University
2012-2023
Fourth Affiliated Hospital of China Medical University
2012-2023
Wenzhou Medical University
2018
Zhejiang University
2018
Affiliated Eye Hospital of Wenzhou Medical College
2018
He Eye Hospital
2013
Institut de génétique et de développement de Rennes
2013
University of Southern California
2008-2012
Doheny Eye Institute
2012
Abstract. Purpose: To determine whether bilateral implantation of an aspheric apodized diffractive multifocal intraocular lens (IOL) with +3.00 diopters (D) results in optical adverse effects compared monofocal IOL. Methods: In a prospective, randomized study 204 eyes (102 patients) AcrySof ReSTOR SN6AD1 IOL or IQ SN60WF IOL, binocular uncorrected (UDVA) and corrected distance visual acuities (CDVA), (UIVA) distance‐corrected intermediate (DCIVA), (UNVA) near (DCNVA), defocus curve,...
To evaluate the effects of anterior capsular opening size on deviation from predicted refraction and effective position intraocular lens (ELP) in cataract surgery. Nonrandomized clinical trial. Eighty patients (80 eyes) with simple age-related cataracts were treated May 2018 to September at Fourth Affiliated Hospital China Medical University. All undergoing phacoemulsification received based voluntary principle. Forty eyes implanted C-loop haptic (AMO Tecnis ZCB00) while other 40 plate (CT...
Complex molecular interactions dictate the developmental steps that lead to a mature and functional cornea lens. Peters anomaly is one subtype of anterior segment dysgenesis especially due abnormal development MSX2 was recently implicated as potential gene critical for development. However, role within complex mechanisms eye remains elusive. Our present study observed morphologic changes in conventional Msx2 knockout (KO) mice found phenotypes consistent with microphthalmia seen humans. The...
Autophagy is a major intracellular degradation system that plays an important role in several biological processes. Although some studies indicate autophagy may play lens and cataracts formation, its underlying mechanism remains to be elucidated. Autophagy‑related gene 4a (ATG4a) cleaves autophagy‑related protein 8 (Atg8) near the C terminus, allowing Atg8 conjugate with phosphatidylethanolamine via exposed glycine; although this pivotal cancer development, no study has yet linked it eye...
Inherited mitochondrial mutations can result in dysfunction or stochastic oxidative damage. Cumulative damage is an important factor age-related disorders, such as cataracts and macular degeneration. Mfn2 mediates the fusion of mitochondria contribute to dynamic balance between fission that determines morphology. We report here conditional loss function head surface ectoderm leads a range congenital eye defects, including small, opacified lens small eyeball most severe phenotypes. The Le-Cre...
The efficacy and safety of 3% diquafosol sodium eye drops in Chinese patients with dry the real-world setting remains unclear.3099 symptoms were screened according to Asia Dry Eye Society latest recommendation. Among them, 3000 enrolled for a phase IV study. We followed up multiple clinical characteristics including corneal fluorescein staining, tear break time, Schirmer's tests, visual acuity, intraocular pressure, others. follow ups performed at baseline, 2 weeks 4 after treatment.Based on...
ELL-associated factor 2 (Eaf2) has an important role in crystalline lens development and maturation; however, its ultraviolet radiation (UV)-induced cataract formation remained elusive. The present study compared UV-induced cell apoptosis, activation of caspase-3 caspase-9 changes protein expression levels B-cell lymphoma (bcl-2), bcl-2-associated X (bax) phosphorylated extracellular signal-regulated kinase wild-type Eaf2-knockout mice. results showed that Eaf2 knockout can reduce apoptosis...
To explore the molecular mechanisms in lens development and pathogenesis of Peters anomaly Smad4 defective mice.Le-Cre transgenic mouse line was employed to inactivate surface ectoderm selectively. Pathological techniques were used reveal morphological changes anterior segment eye. Immunohistochemical staining observe expression E-cadherin, N-cadherin α-SMA mice control at embryonic (E) day 16.5. Real-time quantitative polymerase chain reaction (qPCR) performed detect Snail, Zeb1, Zeb2...
Microphthalmia is characterized by abnormally small eyes and usually retinal dysplasia, accounting for up to 11% of the blindness in children. Right now there no effective treatment disease, underlying mechanisms, especially how dysplasia develops from microphthalmia whether it depends on signals lens ectoderm are still unclear. Mutations genes TGF-β superfamily have been noted patients with microphthalmia. Using conditional knockout mice, here we address question that ocular surface...
Eaf2 encodes a tumor suppressor that plays multiple functions in transcriptional activation, apoptosis, and embryonic development. In this study, we utilized GFP-EAF2 fusion protein to describe the dynamic subcellular movement of Eaf2. is preferentially localized nucleus, presence ELL, it accumulates nuclear speckles. However, an unstable whose stability affected by serum. Further, provided first evidence distribution responsive DNA damage. Following UV irradiation, relocalized nucleolus,...
Objective To investigate the influence of Msx2 conditional gene knockout during lens development in mice. Methods Lens-specific mice were generated using Cre-loxP system. The eyes ( Msx2CKO) and wild-type Msx2WT) examined embryonic early postnatal periods histological, immunofluorescence, situ hybridization, cell proliferation, apoptosis, mRNA microarray analyses. Results Msx2CKO exhibited small formation microphthalmia after birth, while embryos a persistent stalk, formation,...
To investigate the mutually inductive interactions that occur between lens and retinal tissue during development of vertebrate eye.Cre-positive mice were mated with Cre-negative to generate 50% Cre-positive (conditional knockout, CKO) offspring (wild type, WT). The embryos fixed in 4% paraformaldehyde, embedded paraffin, sectioned a thickness 4µm. sections processed for hematoxylin eosin staining. primary antibody used immunofluorescence detection was sc-9305 bone morphogenetic proteins...
To investigate the influence of bone morphogenetic protein type IA receptor [BMPR-IA (ALK3)] conditional knockout in lens on expression 4 (BMP4) during development vertebrate eye.Cre-positive mice were mated with Cre-negative to generate 50% Cre-positive (conditional knockout, CKO) embryos, 8 eyes and offspring (wild type, WT) eyes. The embryos fixed 4% paraformaldehyde, embedded paraffin, sectioned a thickness µm. Removal paraffin wax dehydrating for sections, then procedure situ...
AIM: To study whether specific anesthetic drugs or tear layer evaporation was primarily responsible for the acute cataract and what change of lens structure is in anesthetized mice. METHODS: Five groups were set up experiment: Group A (topicamide phenylephrine mixed eye drop+ chloral hydrate), B (tropicamide drop+sevoflurane), C drop), D drop+chloral hydrate, carbomer drop right eyes), E drop+sevoflurane, eyes). simple classification system used to assess severity opacity. And a numerical...
To investigate the effects of Msx2 on lens epithelium cell cycle, and evaluate changes proliferation, apoptosis cells.Mice cells were cultured transfected with pEGFP-Msx2 control. Msx2-deficient mice (Msx2(-/-) ) tissue isolated. Lens prepared for mRNA extraction using Trizol reagent. CyclinD1 Prox1 expression evaluated by real-time RT-PCR. BrdU incorporation rate investigated immunofluorescence flow cytometry analysis.After pEGFP-Msx2, failed to incorporate anti-phospho-histone-3 detect...
Purpose This study performs to evaluate the Hydrophobic and Hydrosmart 360°square-edge intraocular lens drug delivery of Aspirin using an in vitro capsular model.Methods Cell counting kit-8 assay was used calculate 50% inhibiting concentration values both SRA01/04 HLE-B3 cells. Hoechst staining transwell were detect cell proliferation migration. The capsule model established mainly with a special transwell-col climbing sheet, which TGF-β2 added. ultraviolet spectrophotometer measure...