A5058106647- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Connexins and lens biology
- RNA regulation and disease
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- interferon and immune responses
- Developmental Biology and Gene Regulation
- Genetics and Neurodevelopmental Disorders
- Corneal Surgery and Treatments
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Iron Metabolism and Disorders
- Peptidase Inhibition and Analysis
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- TGF-β signaling in diseases
- Phytochemical compounds biological activities
- Invertebrate Immune Response Mechanisms
- HIV Research and Treatment
- Heat shock proteins research
- FOXO transcription factor regulation
- Calpain Protease Function and Regulation
- Retinal Imaging and Analysis
- Dendrimers and Hyperbranched Polymers
Shandong First Medical University
2024
Sanya University
2024
Tongji University
2023
Shanghai Pulmonary Hospital
2023
Jilin Agricultural University
2023
Exact Sciences (United States)
2023
Sinovac Biotech
2016-2017
Beijing VDJBio (China)
2016
China Medical University
2013
Peking University
2012
Transforming growth factor (TGF)-beta signalling plays important roles in regulating lung development. However, the specific regulatory functions of TGF-beta developing epithelial versus mesenchymal cells are still unknown. By immunostaining, expression pattern type II receptor (TbetaRII) was first determined mouse lung. The TbetaRII were then by conditionally knocking out epithelium floxed-TbetaRII/surfactant protein C-reverse tetracycline transactivator/TetO-Cre mice mesenchyme...
Bone morphogenetic protein 4 (BMP4) is a potent growth factor that involved in many important biological processes. Regulation of the level secreted mature BMP4 determines effects on cells local microenvironment. Previous studies suggested Gremlin, member DAN family proteins, antagonizes activity by sequestering extracellular BMP4. Herein, we report novel intracellular regulatory mechanism which Gremlin interacts with precursor, prevents secretion BMP4, and therefore inhibits more...
Age-related macular degeneration (AMD) is a leading cause of irreversible central blindness among the elderly worldwide. We use exome sequencing to analyse nonsynonymous single-nucleotide variants (SNVs) across whole genome 216 neovascular AMD cases and 1,553 controls. As follow-up validation, we evaluate 3,772 6,942 controls from five independent cohorts in East Asian population. Here show strong evidence an association at novel, missense SNV, rs7739323, which located ubiquitin protein...
Transcription activator-like effector nuclease (TALEN) represents a valuable tool for genomic engineering due to its single-nucleotide precision, high activity, and low cytotoxicity. We report here systematic design characterization of 28 novel TALENs targeting multiple regions CCR5 gene (CCR5-TALEN) which encodes the co-receptor critical entry human immunodeficiency virus type I (HIV-1). By systemic these CCR5-TALENs, we have identified one (CCR5-TALEN-515) with higher specificity, lower...
Gene editing in non-human primates may lead to valuable models for exploring the etiologies and therapeutic strategies of genetically based neurological disorders humans. However, a monkey model that closely mimics pathological behavioral deficits humans has not yet been successfully generated. Microcephalin 1 (MCPH1) is implicated evolution human brain, MCPH1 mutation causes microcephaly accompanied by mental retardation. Here we generated cynomolgus (Macaca fascicularis) carrying biallelic...
A TALE of two assays: Transcription activator-like effectors (TALEs) are programmable proteins that can specifically recognize a DNA sequence. Previous strategies for the synthesis TALEs were complicated and time-consuming. The solid-phase strategy demonstrated here (see scheme) allows quick simple purification ligation product.
Ferroptosis, an iron-triggered modality of cellular death, has been reported to closely relate human aging progression and aging-related diseases. However, the involvement ferroptosis in development maintenance senescent cells still remains elusive. Here, we established a doxorubicin-induced HSkM cell model found that both iron accumulation lipid peroxidation increase cells. Moreover, such overload changed expression panel ferroptosis-response proteins. Interestingly, does not trigger...
This study aimed to determine whether E prostanoid receptor-3 (EP3) is involved in prostacyclin (PGI2)-evoked vasoconstrictor activity of resistance arteries and if so, how it changes under hypertensive conditions. Mesenteric from Wistar-Kyoto rats (WKYs) spontaneously (SHRs) were isolated for functional biochemical studies. Here we show that vessels WKYs, PGI2 or the endothelial muscarinic agonist ACh (which stimulates vitro synthesis) evoked activity, which increased SHRs. The...
Liver cancer is one of the most lethal types in humans, but our understanding molecular mechanisms underlying this process remains insufficient. Here, we conducted high-content screening potential genes involved liver metastasis, which selected from Kyoto Encyclopedia Genes and Genomes (KEGG) pathway database, based on SAMcell method RNA interference technology. We identified two powerful metastasis process, AEG-1 AKR1C2, both proved to be positive regulators promoting cancer. Further...
Abstract Eaf2 , E LL‐ a ssociated f actor 2, encodes protein that is homologous to the human EAF1 which was shown interact with transcriptional elongation factor MEN/ELL. During mouse embryogenesis, preferentially expressed in central nervous system and sensory neuroendocrine organs, including brain, spinal cord, cranial ganglia, developing otocyst, retina, pituitary. transcripts were also found sites where active epithelium–mesenchymal interactions are occurring. These included invaginating...
Recent effective use of TAL Effectors (TALEs) has provided an important approach to the design and synthesis sequence-specific DNA-binding proteins. However, it is still a challenging task manufacture TALE modulators because limited knowledge TALE–DNA interactions. Here we synthesized more than 200 identified two determining factors transcription activity in vivo: chromatin accessibility distance from start site. The implementation these gain-of-function screen was successfully demonstrated...
Eaf2 encodes a tumor suppressor that plays multiple functions in transcriptional activation, apoptosis, and embryonic development. In this study, we utilized GFP-EAF2 fusion protein to describe the dynamic subcellular movement of Eaf2. is preferentially localized nucleus, presence ELL, it accumulates nuclear speckles. However, an unstable whose stability affected by serum. Further, provided first evidence distribution responsive DNA damage. Following UV irradiation, relocalized nucleolus,...
So viel einfacher: Transkriptionsaktivator-ähnliche Effektoren (TALEs) sind programmierbare Proteine, die eine DNA-Sequenz spezifisch erkennen. Frühere Ansätze zur Synthese von TALEs waren schwierig und zeitaufwendig. Die hier vorgestellte Festphasensynthesestrategie (siehe Schema) ermöglicht schnelle einfache Reinigung des Ligationsprodukts.