A5101689271- Neonatal Respiratory Health Research
- Fibroblast Growth Factor Research
- Congenital Diaphragmatic Hernia Studies
- TGF-β signaling in diseases
- Developmental Biology and Gene Regulation
- Renal and related cancers
- Congenital heart defects research
- Pluripotent Stem Cells Research
- Protease and Inhibitor Mechanisms
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Pulmonary Hypertension Research and Treatments
- Immune Cell Function and Interaction
- S100 Proteins and Annexins
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Cancer, Hypoxia, and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Bacillus and Francisella bacterial research
- Connective tissue disorders research
- Cancer Cells and Metastasis
- IL-33, ST2, and ILC Pathways
- MicroRNA in disease regulation
- Neuroscience of respiration and sleep
- Cancer-related Molecular Pathways
Children's Hospital of Los Angeles
2010-2021
University of Southern California
1988-2016
APLA Health
2013
The University of Queensland
1993
Medical Council of Canada
1993
University of British Columbia
1993
Martin Luther King, Jr. Multi-Service Ambulatory Care Center
1992
University of California, Los Angeles
1991-1992
Stony Brook School
1992
MRC Protein Phosphorylation and Ubiquitylation Unit
1988
The role of stem cells in regenerative medicine is evolving rapidly. Here, we describe the application, for kidney regeneration, a novel non-genetically modified cell, derived from human amniotic fluid. We show that these pluripotent can develop and differentiate into de novo structures during organogenesis vitro.Human fluid-derived (hAFSCs) were isolated male fluid obtained between 12 18 weeks gestation. Green fluorescent protein Lac-Z-transfected hAFSCs microinjected murine embryonic...
Fibroblast growth factor-10 (FGF10) controls survival, proliferation, and differentiation of distal-alveolar epithelial progenitor cells during lung development.To test for the protective regenerative effect Fgf10 overexpression in a bleomycin-induced mouse model pulmonary inflammation fibrosis.In SP-C-rtTA; tet(O)Fgf10 double-transgenic mice, fibrosis was induced 2-month-old transgenic mice by subcutaneous delivery bleomycin (BLM), using an osmotic minipump 1 week. Exogenous expression...
Epithelial to mesenchymal transition (EMT) is a key process during embryonic development and disease progression. During EMT, epithelial cells lose features express cell markers, which correlate with increased migration invasion. Transforming growth factor-β (TGF-β) multifunctional cytokine that induces EMT in multiple types. The TGF-β pathway regulated by microRNAs (miRNAs), are small non-coding RNAs regulating the translation of specific messenger RNAs. Herein, we identified mir-99a...
Both nature and induced regulatory T (Treg) lymphocytes are potent regulators of autoimmune allergic disorders. Defects in endogenous Treg cells have been reported patients with asthma, suggesting that disrupted cell-mediated immunological regulation may play an important role airway inflammation. In order to determine whether adoptive transfer generated vitro can be used as effective therapeutic approach suppress inflammation, exogenously were infused into ovalbumin-sensitized mice prior or...
We examined possible roles of keratinocyte growth factor (KGF) and hepatocyte (HGF) in lung morphogenesis. By polymerase chain reaction, transcripts for both KGF its receptor were detected early (rat gestational days 16 14, respectively) their abundance increased during To evaluate role morphogenesis, day 14 explants cultured Dulbecco's modified Eagle medium + 10% fetal calf serum 1 to 4 the presence (5-50 ng/ml) or absence (control). (at 25 50 induced a marked reduction number terminal...
Abstract Dynamic regulation of intestinal cell differentiation is crucial for both homeostasis and the response to injury or inflammation. Sprouty2, an intracellular signaling regulator, controls pathways including PI3K MAPKs that are implicated in dysregulated inflammatory bowel disease. Here, we ask whether Sprouty2 secretory colitis. We report colonic epithelial deletion leads expanded tuft goblet populations. loss induces PI3K/Akt signaling, leading GSK3β inhibition interleukin (IL)-33...
Bone morphogenetic protein 4 (BMP4) is a potent growth factor that involved in many important biological processes. Regulation of the level secreted mature BMP4 determines effects on cells local microenvironment. Previous studies suggested Gremlin, member DAN family proteins, antagonizes activity by sequestering extracellular BMP4. Herein, we report novel intracellular regulatory mechanism which Gremlin interacts with precursor, prevents secretion BMP4, and therefore inhibits more...
Lung cancer is the most common visceral malignancy in males, with rapidly increasing incidence females, and a devastatingly poor prognosis. Transforming growth factor (TGF)-beta has been shown to induce senescence A549 lung cells, both TGF-beta bone morphogenetic protein (BMP) 2 can suppress transformed phenotype of cells vitro. We examined effects BMP4, another member superfamily, on specific oncogenic properties cells. When were treated continuously 100 ng/ml senescent was observed after...
Parasympathetic signaling has been inferred to regulate epithelial branching as well organ regeneration and tumor development. However, the relative contribution of local nerve contact versus secreted signals remains unclear. Here, we show a conserved (vertebrates invertebrates) requirement for intact nerves in airway branching, persisting even when cholinergic neurotransmission is blocked. In vertebrate lung, deleting enhanced green fluorescent protein (eGFP)-labeled intrinsic neurons using...
Pulmonary antioxidant enzyme ontogeny has been reported in species with a relatively short gestation such as hamsters, rats, rabbits, and guinea pigs. We examined the of system together surfactant phospholipid disaturated phosphatidylcholine (DSPC) fetal lamb lung (term is 148 days). Lung tissue from 36 fetuses gestational ages ranging 121 to 145 days were assayed for DSPC content activities three enzymes: superoxide dismutase, catalase, glutathione peroxidase. Between dismutase activity...
Early embryonic lung branching morphogenesis is regulated by many growth factor-mediated pathways. Bone morphogenetic protein 4 (BMP4) one of the morphogens that stimulate epithelial in mouse explant culture. To further understand molecular mechanisms BMP4-regulated development, we studied biological role Smad-ubiquitin regulatory factor 1 (Smurf1), an ubiquitin ligase specific for BMP receptor-regulated Smads, during development. The temporo-spatial expression pattern Smurf1 was first...
Neovascularization is a key regulatory process in fetal growth and development. Although factors promoting development of the pulmonary vasculature have been investigated, nothing known regarding molecular mechanisms that may counteract these stimuli. Endothelial monocyte-activating polypeptide (EMAP) II has recently identified as an antiangiogenic factor tumor vascular We postulated EMAP putative negative modulator lung growth. mRNA protein decrease fivefold ( P < 0.01) developing lungs...
A putative mitogen-activated protein kinase (MAPK) has recently been identified, which potentially phosphorylates the human epidermal growth factor (EGF) receptor at a physiological site (Thr-669) and is distinguished from other MAPKdextracellular signal-regulated kinases (ERKs) on basis of chromatographic, immunological, kinetic data.Here we report that this newly discovered MAPK physically associated with EGF in A431 cells related receptor/tyrosine HEW (encoded by c-neu) enzyme...