A5050597104- Animal Genetics and Reproduction
- Congenital heart defects research
- Animal Nutrition and Physiology
- Livestock and Poultry Management
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Developmental Biology and Gene Regulation
- Advanced Fluorescence Microscopy Techniques
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- Cell Image Analysis Techniques
- T-cell and B-cell Immunology
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Cellular Mechanics and Interactions
- Molecular Biology Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Proteoglycans and glycosaminoglycans research
- Monoclonal and Polyclonal Antibodies Research
- Coronary Artery Anomalies
- Molecular Junctions and Nanostructures
- Axon Guidance and Neuronal Signaling
- Pluripotent Stem Cells Research
University of Southern California
2013-2024
Children's Hospital of Los Angeles
2013-2024
California Institute for Regenerative Medicine
2022
Imaging Center
1999-2019
California Institute of Technology
2005-2015
Bioengineering Center
2009-2011
Pasadena City College
2009
Alltech (United States)
2006
Columbia University
1990-1998
Howard Hughes Medical Institute
1990-1998
We describe a system to evaluate the function of lymphocyte-specific and generally expressed genes in differentiation and/or lymphocytes. RAG-2 (recombination-activating gene 2)-deficient mice have no mature B T lymphocytes due inability initiate VDJ recombination. Blastocysts from RAG-2-deficient generate animals with cells following implantation into foster mothers. However, injection normal ES blastocysts leads generation somatic chimeras all which derive injected (referred as blastocyst...
In situ hybridization methods are used across the biological sciences to map mRNA expression within intact specimens. Multiplexed experiments, in which multiple target mRNAs mapped a single sample, essential for studying regulatory interactions, but remain cumbersome most model organisms. Programmable amplifiers based on mechanism of chain reaction (HCR) overcome this longstanding challenge by operating independently enabling multiplexed experiments be performed with an experimental timeline...
Immunoglobulin (Ig) heavy chain (HC) class switch recombination (CSR) is a late B cell process that involves intrachromosomal DNA rearrangement. Ku70 and Ku80 form end-binding complex required for double strand break repair V(D)J recombination. Ku70−/− (K70T) mice, like activating gene (RAG)-1– or RAG-2–deficient (R1T R2T) have impaired T development at an early progenitor stage, which thought to result least in part from defective (Gu, Y., K.J. Seidl, G.A. Rathbun, C. Zhu, J.P. Manis, N....
To study the influence of immunoglobulin heavy-chain (HC) and light-chain (LC) expression in promoting B-cell differentiation, we have introduced functional HC and/or LC transgenes into recombinase activating gene-2-deficient background (RAG-2-/-). RAG-2-/- mice do not undergo endogenous V(D)J rearrangement events and, therefore, are blocked B- T-cell development at early pro-B- pro-T-cell stages. Introduction promotes a B-lineage cell population that phenotypically has characteristics pre-B...
Background One of the least understood and most central questions confronting biologists is how initially simple clusters or sheet-like cell collectives can assemble into highly complex three-dimensional functional tissues organs. Due to limits oxygen diffusion, blood vessels are an essential ubiquitous presence in all amniote Vasculogenesis, de novo self-assembly endothelial (EC) precursors tubes, first step vessel formation [1]. Static imaging vitro models wholly inadequate capture many...
The <i>Journal of Biomedical Optics</i> (JBO) is a Gold Open Access journal that publishes peer-reviewed papers on the use novel optical systems and techniques for improved health care biomedical research.
Abstract Background The Japanese quail ( Coturnix japonica ) is a popular domestic poultry species and an increasingly significant model in avian developmental, behavioural disease research. Results We have produced high-quality genome sequence, spanning 0.93 Gb assigned to 33 chromosomes. In terms of contiguity, assembly statistics, gene content chromosomal organisation, the shows high similarity chicken genome. demonstrate utility this through three diverse applications. First, we identify...
Embryonic axis elongation is a complex multi-tissue morphogenetic process responsible for the formation of posterior part amniote body. How movements and growth are coordinated between different tissues (e.g. neural tube, axial paraxial mesoderm, lateral plate, ectoderm, endoderm) to drive morphogenesis remain largely unknown. Here, we use quail embryos quantify cell behavior tissue during elongation. We tissue-specific contribution by using 3D volumetric techniques, then parameters such as...
The ability to generate transgenic mice has been a powerful tool in studying functional genomics, and much of our knowledge about developmental biology come from the study chicken embryology. Unfortunately, availability molecular genetic techniques, such as transgenics knockouts, limited for biologists using avian animal models. Efforts develop system rapid production chickens have met with many obstacles, including high husbandry costs long generational times. Recently, Japanese quail...
Embryogenesis is the coordinated assembly of tissues during morphogenesis through changes in individual cell behaviors and collective movements. Dynamic imaging, combined with quantitative analysis, ideal for investigating fundamental questions developmental biology involving cellular differentiation, growth control morphogenesis. However, a reliable amniote model system that amenable to rigors extended, high-resolution imaging tracking has been lacking. To address this shortcoming, we...
Abstract The diverse morphology of vertebrate skeletal system is genetically controlled, yet the means by which cells shape skeleton remains to be fully illuminated. Here we perform quantitative analyses cell behaviours in growth plate cartilage, template for long bone formation, gain insights into this process. Using a robust avian embryonic organ culture, employ time-lapse two-photon laser scanning microscopy observe proliferative cells’ during cartilage growth, resulting cellular...
Abstract Research performed on transgenic animals has led to numerous advances in biological research. However, using traditional retroviral methods generate avian research models proved problematic. As a result, experiments aimed at genetic manipulations birds have remained difficult for this popular tool. Recently, lentiviral allowed the production of birds, including Japanese quail ( Coturnix coturnix japonica ) line showing neuronal specificity and stable expression enhanced green...
Parasympathetic signaling has been inferred to regulate epithelial branching as well organ regeneration and tumor development. However, the relative contribution of local nerve contact versus secreted signals remains unclear. Here, we show a conserved (vertebrates invertebrates) requirement for intact nerves in airway branching, persisting even when cholinergic neurotransmission is blocked. In vertebrate lung, deleting enhanced green fluorescent protein (eGFP)-labeled intrinsic neurons using...
A major challenge in biology is to understand how mechanical interactions and cellular behavior affect the shapes of tissues embryo morphology. The extension neural tube paraxial mesoderm, which form spinal cord musculoskeletal system, respectively, results elongated shape vertebrate embryonic body. Despite our understanding each these elongates independently others, morphogenetic consequences their simultaneous growth are still unclear. Our study investigates differential growth, tissue...
We present an archetypal set of three-dimensional digital atlases the quail embryo based on microscopic magnetic resonance imaging (microMRI). The are composed three modules: (1) images fixed ex ovo quail, ranging in age from embryonic day 5 to 10 (e05 e10); (2) a coarsely delineated anatomical atlas microMRI data; and (3) organ system-based hierarchical graph linked delineations. is designed be accessed using SHIVA, free Java application. extensible can contain other types information...