A5051812491- Congenital heart defects research
- Cancer Cells and Metastasis
- Wnt/β-catenin signaling in development and cancer
- Lymphatic System and Diseases
- Developmental Biology and Gene Regulation
- Proteoglycans and glycosaminoglycans research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Animal Genetics and Reproduction
- Cancer-related gene regulation
- Immunotherapy and Immune Responses
- Tissue Engineering and Regenerative Medicine
- Mechanisms of cancer metastasis
- Connective tissue disorders research
- Kruppel-like factors research
- MicroRNA in disease regulation
- Renal and related cancers
- Cell Adhesion Molecules Research
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Cancer Research and Treatments
- Digestive system and related health
- Freezing and Crystallization Processes
- Dietary Effects on Health
- Fibroblast Growth Factor Research
Cornell University
2013-2024
New York State College of Veterinary Medicine
2022-2024
Technion – Israel Institute of Technology
2023
Hudson Institute
2020
McMaster University
2001-2013
Harvard University
2008-2011
The matrix metalloproteinase matrilysin (MMP-7) is expressed in the tumor cells of a majority mouse intestinal and human colonic adenomas.We showed previously that target gene -catenin-Tcf, transcription factor complex whose activity thought to play crucial role initiation tumorigenesis.Here we report overexpression stable mutant form -catenin alone was not sufficient effect expression luciferase from promoter-luciferase reporter plasmid.However, cotransfection with an vector encoding PEA3...
The counterclockwise coiling of the intestines is initiated by a leftward tilt primitive gut tube, imparted left–right asymmetries in architecture dorsal mesentery. In silico analysis suggests that this achieved synergistic changes its epithelium and mesenchyme. Within mesenchymal compartment, cells are more densely packed on left than right. results indicate property can result from both extracellular matrix (ECM) cell:cell adhesion. We find mesentery ECM indeed asymmetric moreover adhesion...
The vertebrate intestine forms by asymmetric gut rotation and elongation, errors cause lethal obstructions in human infants. Rotation begins with tissue deformation of the dorsal mesentery, which is dependent on left-sided expression Paired-like transcription factor Pitx2. conserved morphogen Nodal induces Pitx2 to govern embryonic laterality, but organ-level regulation during asymmetry remains unknown. We found be dispensable for mesentery deformation. Intestinal instead required a...
Expression of Pitx2 on the left side embryo patterns left-right (LR) organs including dorsal mesentery (DM), whose asymmetric cell behavior directs gut looping. Despite importance organ laterality, chromatin-level regulation remains undefined. Here, we show that genes immediately neighboring in chicken and mouse, a long noncoding RNA (Pitx2 locus-asymmetric regulated or Playrr), are expressed right repressed by Pitx2. CRISPR/Cas9 genome editing Playrr, 3D fluorescent situ hybridization...
Mutations in the pathways regulating mammary epithelial stem cell (MESC) self-renewal and differentiation are currently hypothesized to result uncontrolled division and, turn, breast tumor formation. Although research is aggressively being pursued understand how such cancer formation, current studies have been greatly limited by MESC scarcity. To address this issue, study has successfully developed large-scale expansion protocols for through subculture of murine tissue aggregates, called...
Intestinal lacteals are essential lymphatic channels for absorption and transport of dietary lipids drive the pathogenesis debilitating metabolic diseases. However, organ-specific mechanisms linking dysfunction to disease etiology remain largely unknown. In this study, we uncover an intestinal program that is linked left-right (LR) asymmetric transcription factor Pitx2. We show deletion Pitx2 enhancer ASE alters normal lacteal development through lacteal-associated contractile smooth muscle...
Background & AimsThe enteroendocrine cell (EEC) lineage is important for intestinal homeostasis. It was recently shown that EEC progenitors contribute to epithelial growth and renewal, but the underlying mechanisms remain poorly understood. MicroRNAs are under-explored along entire trajectory, comparatively little known about their contributions homeostasis.MethodsWe leverage unbiased sequencing eight different mouse models sorting methods identify microRNAs enriched trajectory. We further...
The key to understanding the evolutionary origin and modification of phenotypic traits is revealing responsible underlying developmental genetic mechanisms. An important organismal trait ray-finned fishes gas bladder, an air-filled organ that, in most fishes, functions for buoyancy control, homologous lungs lobe-finned fishes. critical morphological difference between bladders, which otherwise share many characteristics, general direction budding during development. Lungs bud ventrally...
Intestinal smooth muscles are the workhorse of digestive system. Inside millions finger-like intestinal projections called villi, strands muscle cells contract to propel absorbed dietary fats through adjacent lymphatic vessel, lacteal, sending into blood circulation for energy production. Despite this vital function, how villus form, they assemble alongside lacteals, and repair throughout life remain unknown. Here we combine single-cell RNA sequencing mouse intestine with quantitative...
The tumor-initiating cell (TIC) frequency of bulk tumor populations is one the criteria used to distinguish malignancies that follow cancer stem model from those do not. However, frequencies may be influenced by experimental conditions and extent which tumors have progressed, parameters are not always addressed in studies these cells. We employed limiting dilution transplantation minimally manipulated cells mammary several transgenic mouse models determine their frequency. determined whether...
In this study, first, microRNA atlas (and searchable web server) across all major small intestinal epithelial cell types is presented. We have demonstrated microRNAs that uniquely mark several lineages, including enteroendocrine and tuft. Identification of a key marker mouse secretory progenitor cells, miR-672, which we show deleted in humans. used vivo models to establish miR-152 as specific Paneth are highly understudied terms microRNAs.