A5105458454- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- HIV-related health complications and treatments
- RNA modifications and cancer
- Cardiovascular Issues in Pregnancy
- Atherosclerosis and Cardiovascular Diseases
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Nitric Oxide and Endothelin Effects
- Aortic aneurysm repair treatments
- Advanced biosensing and bioanalysis techniques
- Aortic Disease and Treatment Approaches
- Angiogenesis and VEGF in Cancer
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Reproductive Biology and Fertility
- Genetic Syndromes and Imprinting
- Advanced Nanomaterials in Catalysis
Michigan State University
2017-2025
Baylor College of Medicine
2003-2024
Michigan United
2023-2024
Houston Methodist
2024
Jilin University
2024
Ocean University of China
2024
First Hospital of Jilin University
2024
University of Illinois Urbana-Champaign
2023
University of Nottingham Malaysia Campus
2023
Biocrede (United States)
2018
The polycomb repressive complex 2 (PRC2) is the major methyltransferase for H3K27 methylation, a modification critical maintaining repressed gene expression programs throughout development. It has been previously shown that PRC2 maintains histone methylation patterns during DNA replication in part through its ability to bind H3K27me3. However, mechanism by which recognizes H3K27me3 unclear. Here we show WD40 domain of EED, component, methyllysine histone-binding domain. crystal structures...
Tudor domains are protein modules that mediate protein-protein interactions, potentially by binding to methylated ligands. A group of germline specific single and multiTudor domain containing proteins (TDRDs) represented drosophila its mammalian orthologs Tdrd1, Tdrd4/RNF17, Tdrd6 play evolutionarily conserved roles in germinal granule/nuage formation germ cell specification differentiation. However, their physiological ligands, the biochemical structural basis for ligand recognition,...
Arginine methylation modulates diverse cellular processes and represents a molecular signature of germ-line-specific Piwi family proteins. A subset Tudor domains recognize arginine modifications, but the binding mechanism has been lacking. Here we establish that, like other germ-line proteins, ancestral staphylococcal nuclease domain-containing 1 (SND1) polypeptide is expressed associates with PIWIL1/Miwi in germ cells. We find that human SND1 binds PIWIL1 an methylation-dependent manner...
Modular protein domains are functional units that can be modified through the acquisition of new intrinsic activities or by formation novel domain combinations, thereby contributing to evolution proteins with biological properties. Here, we assign groups related compositions and properties, termed "domain clubs," which use compare multiple eukaryotic proteomes. This analysis shows different types take distinct evolutionary trajectories, correlate conservation, gain, expansion, decay...
Piwi-interacting RNAs are small regulatory with key roles in transposon silencing and regulation of gametogenesis. The production mature piwi-interacting requires a critical step trimming RNA intermediates to achieve optimally sized RNAs. poly(A)-specific ribonuclease family deadenylase PNLDC1 is implicated silkworms. physiological function mammals remains unknown. Using Pnldc1-deficient mice, here we show that required for biogenesis, silencing, spermatogenesis. Pnldc1 mutation mice...
Abstract Pachytene piRNAs are the most abundant in mammalian adult testes. They generated from long precursor transcripts by primary piRNA biogenesis pathway but factors involved pachytene precursors processing poorly understood. Here we show that Tudor domain-containing 5 (TDRD5) protein is essential for mice. Conditional inactivation of TDRD5 mouse postnatal germ cells reveals selectively regulates production piRNA-producing precursors, with little effect on low-abundant piRNAs....
Over 75% of failed pregnancies involve implantation defects. Growth factor receptor-bound protein 2 (GRB2) is an adaptor involved in signal transduction and cell communication. Here we show that the expression GRB2 lower endometrium infertile women with endometriosis compared to controls. Our mouse model revealed development results loss eutopic endometrium. To understand role uterus, generated mice conditional ablation Grb2 Pgr positive cells (Grb2d/d). Grb2d/d were due failure. Although...
Fibroblast growth factor-10 (FGF-10) is a stromal-derived paracrine factor considered to be important during embryogenesis; however, its expression by cells in the female reproductive tract has not been investigated. Therefore, an ovine FGF-10 cDNA was cloned from endometrial library investigate and potential characteristics of uterus. The encodes protein 213 amino acids possesses unusually long 5′ untranslated region (UTR). In situ hybridization demonstrated that mRNA expressed stromal...
Abstract The intermitochondrial cement (IMC) and chromatoid body (CB) are posited as central sites for piRNA activity in mice, with MIWI initially assembling the IMC processing before translocating to CB functional deployment. regulatory mechanism underpinning translocation, however, has remained elusive. We unveil that loading is trigger translocation from CB. Mechanistically, facilitates release by weakening its ties mitochondria-anchored TDRKH. This, turn, enables arginine methylation of...
Rho family guanosine triphosphatase (GTPase) 3 (Rnd3), a member of the small GTPase family, has been suggested to regulate cell actin cytoskeleton dynamics, migration, and apoptosis through kinase-dependent signaling pathway. The biological function Rnd3 in heart is unknown. downregulation transcripts was found patients with end-stage failure. pathological significance loss transition failure remains unexplored. To investigate functional consequence associated molecular mechanism, we...
The P-element-induced wimpy testis (PIWI)-interacting RNA (piRNA) pathway plays a central role in transposon silencing and genome protection the animal germline. A family of Tudor domain proteins regulates piRNA through direct domain-PIWI interactions. domains are known to fulfill this function by binding methylated PIWI an arginine methylation-dependent manner. Here, we report mechanism methylation-independent interaction. Unlike most other domains, extended mammalian domain-containing...
Polycomb group (PcG) proteins are a subset of epigenetic factors that highly conserved throughout evolution. In mammals, PcG can be classified into two muti-proteins complexes: repressive complex 1 (PRC1) and PRC2. Increasing evidence has demonstrated complexes play critical roles in the regulation gene expression, genomic imprinting, chromosome X-inactivation, chromatin structure. Accordingly, dysfunction is tightly orchestrated with abnormal developmental processes. Here, we summarized...
Epithelial-mesenchymal interactions (EMI) are necessary for epithelial cell proliferation, differentiation, and function in the uterus mediated, part, by paracrine growth factors of stromal origin. The objective this study was to determine if hepatocyte factor (HGF, scatter factor) its receptor c-met were present ovine characterize their temporal spatial expression during estrous cycle pregnancy. Reverse transcription-polymerase chain reaction used clone partial cDNAs HGF from endometrial...
LOTUS domains are helix-turn-helix protein folds identified in essential germline proteins and conserved prokaryotes eukaryotes. Despite originally predicted as an RNA binding domain, its molecular activity towards is controversial. In particular, the most property for domain family remains unknown. Here, we uncovered unexpected specific interaction of with G-rich sequences. Intriguingly, exhibit high affinity to G-quadruplex tertiary structures implicated diverse cellular processes...
The cytoplasmic droplet is a conserved dilated area of cytoplasm situated at the neck sperm flagellum. Viewed as residual inherited from late spermatids, contains numerous saccular elements its key content. However, origin these saccules and function have long been speculative. Here, we identify molecular components by uncovering vesicle pathway essential for formation sequestration within droplet. This process governed transmembrane protein SYPL1 interaction with VAMP3. Genetic ablation in...
In sheep, uterine development begins during fetal life but is only completed postnatally with proliferation and branching morphogenetic differentiation of the endometrial glandular epithelium (G) from luminal (L) between birth or Postnatal Day (PND) 0 PND 56. other epithelial-mesenchymal organs, fibroblast growth factor (FGF)-7 FGF-10, hepatocyte (HGF), insulin-like (IGF)-I IGF-II play essential roles in ductal morphogenesis. Semiquantitative reverse transcription-polymerase chain reaction...
PIWI proteins and their associated piRNAs act to silence transposons promote gametogenesis. Murine MIWI, MILI, MIWI2 have multiple arginine glycine (RG)-rich motifs at N-terminal domains. Despite being known as docking sites for the TDRD family proteins, in vivo regulatory roles these RG directing piRNA biogenesis spermatogenesis remain elusive. To investigate functional significance of mammalian vivo, we genetically engineered an lysine (RK) point mutation a conserved motif MIWI mice. We...