A5062378176- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Estrogen and related hormone effects
- Endometriosis Research and Treatment
- Autophagy in Disease and Therapy
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
- Peroxisome Proliferator-Activated Receptors
- Pregnancy and preeclampsia studies
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Uterine Myomas and Treatments
- Inflammatory mediators and NSAID effects
- Ovarian function and disorders
- Microtubule and mitosis dynamics
- Retinal Diseases and Treatments
- Lipid metabolism and biosynthesis
- RNA Interference and Gene Delivery
- Healthcare and Venom Research
- Birth, Development, and Health
- Renal and related cancers
- Cancer-related molecular mechanisms research
- Metabolomics and Mass Spectrometry Studies
Konkuk University
2014-2025
Konkuk University Medical Center
2006-2021
University of California, San Diego
2021
CHA University
2020
Maria Fertility Hospital
2020
Cincinnati Children's Hospital Medical Center
2011-2014
Nune Eye Hospital
2010
Washington University in St. Louis
2002-2009
University of South Carolina
2007
University of Kansas Medical Center
1996-2002
We have demonstrated previously that cyclo-oxygenase-2 (COX2), the rate-limiting enzyme in biosynthesis of prostaglandins (PGs), is essential for blastocyst implantation and decidualization. However, candidate PG(s) participates these processes mechanism its action remain undefined. Using COX2-deficient mice multiple approaches, we demonstrate herein COX2-derived prostacyclin (PGI2) primary PG Several lines evidence suggest effects PGI2 are mediated by activation nuclear hormone receptor...
ABSTRACT The establishment of a receptive uterine environment is critical for embryonic survival and implantation. One gene that expressed in the uterus during peri-implantation period mice required female fertility homeobox Hoxa-10. Here we characterize periimplantation defects Hoxa-10 mutant females investigate functions anlage morphogenesis adult pregnancy. Examination pregnancy has revealed failure implantation as well resorption embryos early postimplantation period. Morphologic...
Hoxa-10 is an AbdominalB-like homeobox gene that expressed in the developing genitourinary tract during embryogenesis and adult uterus early pregnancy. Null mutation of mouse causes both male female infertility. Defective implantation decidualization resulting from loss maternal function uterine stromal cells cause However, mechanisms by which regulates these events are unknown. We have identified two potential for defects Hoxa-10(−/−) mice. First, PGE2 receptor subtypes, EP3 EP4, aberrantly...
Cytosolic phospholipase A2α (cPLA2α) is a major provider of arachidonic acid (AA) for the cyclooxygenase (COX) system biosynthesis prostaglandins (PGs). Female mice with null mutation Pla2g4a produce small litters and often exhibit pregnancy failures, although cause(s) these defects remains elusive. We show that initiation implantation temporarily deferred in Pla2g4a–/– mice, shifting normal ‘window’ leading to retarded feto-placental development without apparent decidual growth....
Abstract Various mediators, including cytokines, growth factors, homeotic gene products, and prostaglandins (PGs), participate in the implantation process an autocrine, paracrine, or juxtacrine manner. However, interactions among these factors that result successful are not clearly understood. Leukemia inhibitory factor (LIF), a pleiotropic cytokine, was shown to be expressed uterine glands on day 4 morning before is critical this mice. mechanism by which LIF executes its effects remains...
Infertility and spontaneous pregnancy losses are an enduring problem to women's health. The establishment of depends on successful implantation, where a complex series interactions occurs between the heterogeneous cell types uterus blastocyst. Although number genes implicated in embryo-uterine during genetic evidence suggests that only small them critical this process. To obtain global view identify novel pathways we used dual screening strategy analyze expression nearly 10,000 mouse by...
Age-related macular degeneration (AMD) describes the progressive of retinal pigment epithelium (RPE), retina, and choriocapillaris is leading cause blindness in people over 50. The molecular mechanisms underlying this multifactorial disease remain largely unknown. To uncover novel secretory biomarkers related to pathogenesis AMD, we adopted an integrated approach compare proteins identified conditioned medium (CM) cultured RPE cells exosomes derived from CM aqueous humor (AH) AMD patients by...
2-Chloro-10-[3(-dimethylamino)propyl]phenothiazine mono hydrochloride (chlorpromazine; CPZ) is an antipsychotic agent that was originally developed to control psychotic disorders. The cytotoxic properties of the CPZ are well known, but its mechanism action poorly understood. In this study, we investigated role apoptosis and autophagy in CPZ-induced cytotoxicity U-87MG glioma cells. treatment inhibited cell proliferation long-term clonogenic survival. Additionally, triggered autophagy, as...
Delayed implantation, considered a state of suspended animation, is widespread in mammals. Blastocysts under this condition remain dormant for an extended period but resume implantation competence upon favorable conditions. The underlying mechanism by which longevity blastocysts maintained not clearly understood. Using autophagy markers and the well-defined delayed model mice, we show that important utero during implantation. However, prolonged dormancy leads to reduced developmental...
In the mouse, estrogen is essential for blastocyst implantation in progesterone (P4)-primed uterus. The mechanism(s) by which initiates this response still remains elusive. present investigation, using delayed examined differential role of estradiol-17β (E2) and its catechol metabolite 4-hydroxy-E2 (4-OH-E2) uterine activation implantation. conditions were induced ovariectomizing mice on day 4 (day 1 = vaginal plug) pregnancy or pseudopregnancy maintaining them with P4 from days 5–7. binding...
Abstract Various attempts have been made to develop stem cell-based therapy alleviate type I diabetes using animal models. However, it has a question whether human insulin produced from explanted cells is solely responsible for the normoglycemia of diabetic animals. In this study, we isolated neural crest-like eyelid fat and examined their therapeutic potentials diabetes. The adipose-derived (HEACs) displayed characteristics crest cells. Using two-step culture condition combined with...
Autophagy is a major cellular catabolic pathway tightly associated with cell survival. The involvement of autophagy in the prolonged survival blastocysts uterus well established, and it was assumed that ovarian steroid hormones - progesterone (P4) estrogens have important roles regulation autophagy. However, information scarce regarding whether these regulate certain hormone-responsive systems. In this study, we investigated effects estrogen P4 on autophagic response uteri pregnant mice...
The infertility phenotype of cyclooxygenase-2 (Cox-2)-deficient female mice establishes the important role Cox-2 in pregnancy. deficiency results defective ovulation, fertilization, implantation, and decidualization; latter which can be restored part by prostacyclin analog carbaprostacyclin. Uterine expression during early pregnancy shows distinct localization kinetics uterine luminal epithelium underlying stromal cells, suggesting that is tightly regulated. Several intracellular signaling...