A5031768116- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Extracellular vesicles in disease
- Intracranial Aneurysms: Treatment and Complications
- Advanced biosensing and bioanalysis techniques
- Enhanced Oil Recovery Techniques
- Electrospun Nanofibers in Biomedical Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Bacterial biofilms and quorum sensing
- Cancer Cells and Metastasis
- Water Treatment and Disinfection
- S100 Proteins and Annexins
- Microfluidic and Bio-sensing Technologies
- MicroRNA in disease regulation
- Periodontal Regeneration and Treatments
- Neuroscience and Neural Engineering
- Microbial bioremediation and biosurfactants
- Conducting polymers and applications
- Microbial metabolism and enzyme function
- Diabetes Management and Research
University of Calgary
2016-2025
Alberta Bone and Joint Health Institute
2013-2024
Vivekananda Institute of Medical Sciences
2009-2010
Dalhousie University
2006-2008
University of Alberta
2008
Microfluidic devices have been used progressively in biomedical research due to the advantages they offer, such as relatively low-cost, rapid and precise processing, an ability support highly automated analyses. Polydimethylsiloxane (PDMS) polymethylmethacrylate (PMMA) are both biocompatible materials widely microfluidics their desirable characteristics. It is recognized that combining these two particular a single microfluidic device would enable development of increasingly in-demand array...
Human bone marrow-derived mesenchymal stem cells (hBM-MSCs) have potential clinical utility in the treatment of a multitude ailments and diseases, due to their relative ease isolation from patients capacity form many cell types. However, hBM-MSCs are sparse, can only be isolated very small quantities, thereby hindering development therapies. The use microcarrier-based stirred suspension bioreactors expand populations offers an approach overcome this problem. Starting with standard culture...
Glial fibrillary acidic protein (GFAP) is as an intermediate filament expressed by certain cells in the central nervous system (CNS). GFAP has been recognized a reliable biomarker of CNS injury. However, due to absence rapid and easy-to-use assays for detection injury biomarkers, measuring levels identify not attained widespread clinical implementation. In present work, we developed polyethylenimine (PEI) coated graphene screen-printed electrode used it highly sensitive immunosensing GFAP....
Mesenchymal progenitor cells (MPCs) have shown promise initiating articular cartilage repair, with benefits largely attributed to the trophic factors they secrete. These can be found in conditioned medium (CM) collected from cell cultures, and it is believed that extracellular vesicles (EVs) within this CM are at least partially responsible for MPC therapeutic efficacy. This study aimed examine functionality of EV fraction compared whole obtained human adipose-derived MPCs an vivo murine...
Mammalian neural stem cells hold great promise for the treatment of central nervous system disorders. However, to be a viable clinical millions individuals afflicted with these disorders, it is necessary develop cell expansion protocols. Although difficult grow in bioreactors, can expanded carefully designed media as aggregates brain tissue. The objective this study was examine control aggregate size batch culture by manipulating agitation rate, and hence liquid shear oxygen transfer...
Abstract The clinical use of neural precursor cells (NPCs) for the treatment neurological diseases, such as Parkinson's disease and Huntington's disease, requires overcoming scarcity these through controlled expansion. main objective present study was to develop a large‐scale computer‐controlled bioprocess expansion mammalian NPCs in suspension culture by scaling up existing reactor protocols. In order support oxygen demands maximum cell densities achieved, volumetric mass transfer...
Abstract Injury to the spinal cord or peripheral nerves can lead development of allodynia due loss inhibitory tone involved in sensory function. The potential intraspinal transplants GABAergic cells restore and thus decrease pain behaviors a rat model neuropathic was investigated. Allodynia left hind paw induced rats by unilateral L5– 6 nerve root ligation. Mechanical sensitivity assessed using von Frey filaments. Postinjury, transgenic fetal green fluorescent protein mouse human neural...
Human mesenchymal stem cells (hMSCs) are typically obtained for research or therapeutic applications by isolating and subculturing adherent from bone marrow on tissue-culture substrates using growth media. The purity properties of the resulting populations can be affected greatly conditions under which they cultured. Fetal bovine serum (FBS), although ill-defined, has been widely used as a critical requirement conventional hMSC culture. However, defined serum-free medium would facilitate...
Following an ischemic injury to the brain, induction of angiogenesis is critical neurological recovery. The angiogenic benefits mesenchymal stem cells (MSCs) have been attributed at least in part actions extracellular vesicles (EVs) that they secrete. EVs are membrane-bound contain various biomolecules capable eliciting therapeutic responses and relevance cerebral applications due their ability cross blood-brain barrier (BBB). Though MSCs commonly cultured under oxygen levels present...
It has been hypothesized that breast tumor formation results from the activity of a scarce population cells known as Breast Cancer Stem Cells (BrCSCs) and development effective cancer therapies may therefore ultimately rely upon ability to effectively target these for eradication. The scarcity BrCSCs in vivo severely compromises research on populations, analyses are restricted those requiring small cell numbers, become major impediment therapeutic strategies against cancer. Through culture...
The transplantation of in vitro expanded human neural precursor cells (hNPCs) represents a potential new treatment alternative for individuals suffering from incurable neurodegenerative disorders such as Parkinson's disease (PD) and Huntington's (HD). However, order cell restorative therapy to have widespread therapeutic significance, it will be necessary generate unlimited quantities clinical grade hNPCs standardized method. We report here that we developed serum-free medium scale-up...
Abstract The influence of bacterial biomass on hydraulic properties porous media (bioclogging) has been explored as a viable means for optimizing subsurface bioremediation and microbial enhanced oil recovery. In this study, we present pore network simulator modeling biofilm evolution in including hydrodynamics nutrient transport based coupling advection with Fickian diffusion reaction term to account consumption. Biofilm non‐zero permeability permitting liquid flow through the itself. To...
Elevated glial fibrillary acidic protein (GFAP) in the blood serum is one of promising bodily fluid markers for diagnosis central nervous system (CNS) injuries, including traumatic brain injury (TBI), stroke, and spinal cord (SCI). However, accurate point-of-care (POC) quantification GFAP clinical samples has been challenging yet to be clinically validated against gold-standard assays outcome practices. This work engineered characterized a novel nanoporous carbon screen-printed electrode...
Small extracellular vesicles (EVs) have emerged as a focal point of EV research due to their significant role in wide range physiological and pathological processes within living systems. However, uncertainties about the nature these added considerable complexity already difficult task developing EV-based diagnostics therapeutics. Whereas small EVs been shown be negatively charged, surface charge has not yet properly quantified. This gap knowledge made it challenging fully understand...
Mesenchymal stem cells (MSCs) have the capacity to differentiate towards bone, fat, and cartilage lineages. The most widely used culture differentiation protocols for MSCs are currently limited by their use of serum-containing media small-scale static vessels. Suspension bioreactors multiple advantages over vessels (e.g., scalability, control, mechanical forces). This study sought compare formation 3D aggregates human synovial fluid within suspension microwell plates. It also elucidate...