A5012988440- Hearing, Cochlea, Tinnitus, Genetics
- Marine animal studies overview
- Animal Vocal Communication and Behavior
- Biochemical Analysis and Sensing Techniques
- Neuroscience and Neuropharmacology Research
- Hearing Loss and Rehabilitation
- Animal Nutrition and Physiology
- Neural dynamics and brain function
- Vestibular and auditory disorders
- Immune Response and Inflammation
- Noise Effects and Management
- Antimicrobial Peptides and Activities
- Neurobiology and Insect Physiology Research
- Zebrafish Biomedical Research Applications
- Animal Behavior and Reproduction
- Congenital heart defects research
- Acoustic Wave Phenomena Research
- Neurogenesis and neuroplasticity mechanisms
- Underwater Vehicles and Communication Systems
- Ear and Head Tumors
- Cancer-related molecular mechanisms research
- Developmental Biology and Gene Regulation
- Bat Biology and Ecology Studies
- Olfactory and Sensory Function Studies
- RNA Research and Splicing
University of Washington
2015-2024
Merrill (United States)
1996-2016
Chicago Neuropsychology Group
2012
Palmetto Hematology Oncology
2012
University of Washington Medical Center
2005
Seattle University
1987-2004
Neurological Surgery
1990-2004
University of California, Irvine
1971-2004
Creighton University
2002
Institute of Neurobiology
1999-2001
Recovery of hair cells was studied at various times after acoustic trauma in adult quail. An initial loss recovered to within 5 percent the original number cells. Tritium-labeled thymidine injected this determine if mitosis played a role recovery Within 10 days trauma, incorporation [ 3 H]thymidine seen over nuclei and supporting region cell loss. Thus, regeneration can occur embryonic terminal mitosis.
Hearing loss is most often the result of hair-cell degeneration due to genetic abnormalities or ototoxic and traumatic insults. In postembryonic adult mammalian auditory sensory epithelium, organ Corti, no regeneration has ever been observed. However, nonmammalian epithelia are capable regenerating hair cells as a consequence nonsensory supporting-cell proliferation. The supporting Corti highly specialized, terminally differentiated cell types that apparently incapable At molecular level...
• This study examines the temporal pattern of hair cell loss in chick basilar papilla following ten days gentamicin administration hatchling chicks. Chicks were subsequently killed at ages 11, 18, 25, and 32 days. The papillae embedded plastic serially sectioned for light microscopic analysis. Hair counts obtained 100-μm intervals throughout length papilla. Significant was documented basally 11-day-old chicks, spread apically over time to maximal 18-day-old animals. Relative control there a...
Abstract Extracellular recordings of responses to tone‐burst stimulation were used determine the tonotopic organization n. magnocellularis (NM) and laminaris (NL) in hatchling chickens. NM cells show “primary‐like” response patterns ipsilateral stimulation, are arranged dorso‐ventral isofrequency columns. Units responding highest frequency tones (about 4,100 Hz) situated at rostromedial pole medial division. with lower characteristic frequencies (CF's) found successively caudal lateral...
Mechanosensory hair cells within the zebrafish lateral line spontaneously regenerate after aminoglycoside-induced death. Exposure of 5-d-old larvae to 400 μ m neomycin for 1 h results in death almost all cells. Regeneration new is observed by 24 treatment with nearly complete replacement 72 h. Using bromodeoxyuridine incorporation, we show that majority are generated from a transient increase support cell proliferation occurs between 12 and 21 damage. Additional observations reveal two...
Abstract The consequences of cochlea removal on neuron number and soma cross‐sectional area were examined in the second order auditory nucleus (n. magnocellularis) chickens. Both age subjects at time (basilar papilla) (1–66 weeks) survival period (1–45 days) varied. Neuron determined from Nissl stained sections. Additional material was processed to examine relationship ganglion cell loss changes n. magnocellularis. decreased by 25–30% 10–20% ipsilateral chickens operated during first 6 weeks...
Abstract Afferent regulation of neurons in the cochlear nucleus as a function age was investigated at light microscope level. Unilateral cochlea removal performed on Mongolian gerbils three groups: 1, 8, and 20 weeks postnatal. Animals survived for either 2 days or weeks. An additional group neonatally operated animals had prolonged survival 9 The number anteroventral (AVCN) counted, cross‐sectional area measurements large spherical cells AVCN were made. In 1 week old time surgery, there 35%...
Inner ear sensory hair cell death is observed in the majority of hearing and balance disorders, affecting health more than 600 million people worldwide. While normal aging single greatest contributor, exposure to environmental toxins therapeutic drugs such as aminoglycoside antibiotics antineoplastic agents are significant contributors. Genetic variation contributes markedly differences disease progression during susceptibility ototoxic agents. Using lateral line system larval zebrafish, we...
Abstract The tonotopic and topographic organization of the bilateral projection from second‐order auditory neurons nucleus magnocellularis (NM) to laminaris (NL) was examined in young chickens. In one group birds, NM axons which innervate contralateral NL were severed by cutting crossed dorsal cochlear tract at midline. Heavy terminal degeineration confined neuropil area immediately ventral perikaryl lamina. Very little degeneration seen region. a second series animals, characteristic...
Abstract Nucleus laminaris (NL) is a third‐order auditory nucleus in the avian brain stem which received spatially‐segregated binaural inputs from second‐order magnocellular nuclei. The organization of dendritic structure NL was examined Golgi‐impregnated brains hatchling chickens. Quantitative analyses size and number were made camera lucida drawings 135 neurons sampled throughout nucleus. most significant results this study may be summarized as follows: preponderant neuron n. characterized...
We developed a transgenic mouse to permit conditional and selective ablation of hair cells in the adult utricle by inserting human diphtheria toxin receptor ( DTR ) gene into Pou4f3 gene, which encodes cell-specific transcription factor. In wild-type mice, administration (DT) caused no significant cell loss. +/ DT treatment reduced numbers 6% normal 14 days post-DT. Remaining were located primarily lateral extrastriola. Over time, increased these regions, reaching 17% untreated mice 60...
The lateral line sensory system, found in fish and amphibians, is used prey detection, predator avoidance schooling behavior. This system includes cell clusters, called superficial neuromasts, located on the surface of head trunk developing larvae. Mechanosensory hair cells center each neuromast respond to disturbances water convey information brain via ganglia. convenient location mechanosensory body has made a valuable which study damage regeneration. One way measure survival recovery...
Abstract Nucleus magnocellularis (NM) and nucleus laminaris (NL) are, respectively, second‐ third‐order auditory nuclei in the chicken brain stem. In this report morphogenesis of these is examined. The times origin cells were studied with 3 H‐thymidine autoradiography. number each their rostro‐caudal distribution within was determined Nissl‐stained sections at 9, 11, 13, 15, 17, 19, 21 days incubation. For above ages volumes NM NL also calculated planimetrically volume studied. results...
Abstract The time course and specificity of the changes in dendritic morphology following deafferentation were examined nucleus laminaris young chickens. dendrites neurons are segregated into dorsal ventral domains, which innervated separately from ipsilateral contralateral magnocellularis, respectively. Transection crossed cochlear tract deafferents bilaterally without interrupting matching input to dendrites. In 10‐day‐old chicks, atrophy began immediately after transecting tract; 10%...
Abstract The pattern of primary auditory projections to the brain stem young chickens was investigated using terminal degeneration methods and orthograde transport horseradish peroxidase (HRP) or tritiated amino acid. Of particular interest question whether nucleus laminaris (NL) receives afferents. A study silver‐stained patterns in magnocellularis (NM) NL at three intervals following unilateral interruption cochlear nerve revealed that by 48 hours after lesion, degenerating terminals were...