A5089790618- Genetics, Aging, and Longevity in Model Organisms
- Toxoplasma gondii Research Studies
- Peroxisome Proliferator-Activated Receptors
- Immune Cell Function and Interaction
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- Circadian rhythm and melatonin
- Bacteriophages and microbial interactions
- Reproductive Biology and Fertility
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Photosynthetic Processes and Mechanisms
- Neuroendocrine regulation and behavior
- Adipose Tissue and Metabolism
- Microtubule and mitosis dynamics
- Advanced Fluorescence Microscopy Techniques
- Animal Virus Infections Studies
- Biocrusts and Microbial Ecology
- IL-33, ST2, and ILC Pathways
- Lipid Membrane Structure and Behavior
- Developmental Biology and Gene Regulation
- Immune Response and Inflammation
- Molecular Biology Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
Pomona College
2020-2021
California Institute of Technology
1996-2020
Howard Hughes Medical Institute
2012-2015
Doshisha University
2014
University of Chicago
2012
Obihiro University of Agriculture and Veterinary Medicine
1999-2007
Frontier Science Foundation
2005
Biotechnology Research Center
2005
Hokkaido University
1976
In situ hybridization methods are used across the biological sciences to map mRNA expression within intact specimens. Multiplexed experiments, in which multiple target mRNAs mapped a single sample, essential for studying regulatory interactions, but remain cumbersome most model organisms. Programmable amplifiers based on mechanism of chain reaction (HCR) overcome this longstanding challenge by operating independently enabling multiplexed experiments be performed with an experimental timeline...
Autism spectrum disorder (ASD) involves thousands of alleles in over 850 genes, but the current functional inference tools are not sufficient to predict phenotypic changes. As a result, causal relationship most these genetic variants pathogenesis ASD has yet been demonstrated and an experimental method prioritizing missense for further intensive analysis is crucial. For this purpose, we have designed pipeline that uses Caenorhabditis elegans as model screen phenotype-changing inferred from...
Background Drosophila female germline stem cells (GSCs) reside adjacent to a cellular niche that secretes Bone Morphogenetic Protein (BMP) ligands and anchors the GSCs through adherens junctions. The divide asymmetrically such one daughter remains in as GSC, while other is born away from differentiates. However, given BMP signal can be diffusible, it unclear how local extracellular asymmetry sufficient result robust pattern of asymmetric division. Methods Findings Here we show are polarized...
In both metazoan development and metastatic cancer, migrating cells must carry out a detailed, complex program of sensing cues, binding substrates, moving their cytoskeletons. The linker cell in Caenorhabditis elegans males undergoes stereotyped migration that guides gonad organogenesis, occurs with precise timing, requires the nuclear hormone receptor NHR-67. To better understand how this occurs, we performed RNA-seq individually staged dissected cells, comparing transcriptomes from...
Here we report a low-cost and simple wide field-of-view (FOV) on-chip fluorescence-imaging platform, termed fluorescence Talbot microscopy (FTM), which utilizes the self-imaging effect to enable efficient imaging over large directly scalable FOV. The FTM prototype has resolution of 1.2 μm an FOV 3.9 mm × 3.5 mm. We demonstrate capability on fluorescently labeled breast cancer cells (SK-BR-3) human embryonic kidney 293 (HEK) expressing green fluorescent protein.
Cell migration is a common event during organogenesis, yet little known about how temporally coordinated with organ development. We are investigating stage-specific programs of cell using the linker (LC), migratory crucial for male gonadogenesis C. elegans. During L3 and L4 larval stages wild-type males, LC undergoes changes in its position along route, transcriptional regulation unc-5 netrin receptor zmp-1 zinc matrix metalloprotease, morphology. have identified tailless homolog nhr-67 as...
In epithelial collective migration, leader and follower cells migrate while maintaining cell–cell adhesion tissue polarity. We have identified a conserved protein interactors required for cell during simple migration in the developing C. elegans male gonad. LINKIN is previously uncharacterized, transmembrane throughout Metazoa. seven atypical FG–GAP domains extracellular domain, which potentially folds into β-propeller structure resembling α-integrin ligand-binding domain. LNKN-1 localizes...
ABSTRACT Shiga toxin (Stx), a major virulence factor of enterohemorrhagic Escherichia coli , binds to target cells through multivalent interaction between its B-subunit pentamer and the cell surface receptor globotriaosylceramide, resulting in remarkable increase binding affinity. This phenomenon is referred as “clustering effect.” Previously, we developed peptide library that can exert clustering effect identified Stx neutralizers with tetravalent peptides by screening this for...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTDesign and Evaluation of Potent Inhibitors Asparagine-Linked Protein GlycosylationTamara L. Hendrickson, Jeffrey R. Spencer, Mihoko Kato, Barbara ImperialiView Author Information Division Chemistry Chemical Engineering California Institute Technology Pasadena, 91125 Cite this: J. Am. Chem. Soc. 1996, 118, 32, 7636–7637Publication Date (Web):August 14, 1996Publication History Received2 May 1996Published online14 August inissue 1 January...
Acetylcholine (ACh) promotes various cell migrations in vitro, but there are few investigations into this nonsynaptic role of ACh signaling vivo. Here we investigate the function a muscarinic receptor on an epithelial migration
We compared the reactivity of IgG1 and IgG2a antibodies in mouse sera after infection with virulent RH low-virulent S273 Beverley strains Toxoplasma gondii against SAG1 recombinant p30 (rp30) synthetic peptides.Infected serum samples were collected 9 days infection, level total IgG, rp30 protein twenty peptides assessed. The glycosylphosphatidylinositol (GPI) modification site, hydrophilic-hydrophobic structure, transmembrane region secondary structure sequence analyzed using software.The...
The influence of recombinant cell surface SAG1 (rp30) and secretory GRA1 (rp24) antigens (Ag) on T-cell activation cytokine induction in vitro was compared. activity the level IFN-γ, IL-10 IL-12 expression rp30-immunized T cells were considerably increased presence rp30 Ags. IgG2a IgG1 antibodies (Ab) detected sera rp24- mice, with rp24 Ag having induced significantly higher titer Ab. In vitro<i>,</i> greater antigenicity notable based activation, synthesis suggestive...
We recently reported the role of type 2 innate lymphoid cells (ILC2s) in central nervous system (CNS) demyelination using a model CNS involving recombinant herpes simplex virus 1 (HSV-1) that constitutively expresses mouse interleukin (HSV-IL-2). In this investigation, we studied how ILC2s respond to HSV-IL-2 at cellular level cytokine and gene expression profiling. infected with expressed higher levels granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-5, IL-6, IL-13, IP-10,...
Abstract Cell migration is an important process occurring during normal animal development but also in the early stages of metastatic cancer when cells invasively migrate out primary tumor. It therefore likely that some same genes function both migrations. Although transcriptional profiling has identified many differentially expressed between metastases and tumors, we do not understand most these either or disease states. Our goal been to identify conserved used mammalian metastasis C....
In glioma cells, tumor necrosis factor alpha (TNFa) was reported to increase the number of epidermal growth receptor (EGFR) that mediates EGF-dependent cell proliferation. On other hand, ligands for peroxisome proliferator-activated (PPARa) have been inhibit proliferation various cells. this study, we evaluated influence synthetic PPARa ligand, Wy14643, on basal and TNFa-dependent expression EGFR in U251MG cells derived from a human malignant glioma. Northern blot analysis revealed TNFa...
The fluorescence microscope is one of the most important tools in modern clinical diagnosis and biological science. However, its expense, size limited field-of-view (FOV) are becoming bottlenecks key applications such as large-scale phenotyping low-resource-setting diagnostics. Here we report a low-cost, compact chip-scale fluorescence-imaging platform, termed Fluorescence Talbot Microscopy (FTM), which utilizes self-imaging effect to enable efficient imaging over large directly-scalable...
Cell migrations are vital for the development of multicellular organisms and require precise spatial temporal regulation. Interestingly, migratory cell itself undergoes change during its migration, so is an important consideration in examining dynamic biology. We studying male linker cell, individual C. elegans that directs gonad by carrying out a complex, long‐range migration. During mid‐third larval stage, we observed downregulation one two netrin receptors, unc‐5 . show this required to...