A5001535536- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Microbial infections and disease research
- Liver Disease and Transplantation
- Proteoglycans and glycosaminoglycans research
- Veterinary medicine and infectious diseases
- Animal Disease Management and Epidemiology
- Multiple Myeloma Research and Treatments
- Liver physiology and pathology
- Virology and Viral Diseases
- Aquaculture disease management and microbiota
- Antibiotic Resistance in Bacteria
- interferon and immune responses
- Bacillus and Francisella bacterial research
- Hepatitis B Virus Studies
- Rabies epidemiology and control
- Insect symbiosis and bacterial influences
- Parasitic Infections and Diagnostics
- Liver Diseases and Immunity
- Bacterial Infections and Vaccines
- Plant Pathogenic Bacteria Studies
- Animal health and immunology
Long Island University
2022-2025
Virginia–Maryland College of Veterinary Medicine
2010-2018
Virginia Tech
2009-2018
National Institutes of Health
2008-2012
National Institute of Allergy and Infectious Diseases
2008-2012
Universidade Federal do Rio de Janeiro
2008
Chinese Academy of Agricultural Sciences
2002
Harbin Veterinary Research Institute
2002
Aarhus University Hospital
2000
Norwegian University of Science and Technology
2000
Previous studies of epithelial immune responses to rotavirus infection have been conducted in transformed cell lines. In this study, we evaluated a non-transformed porcine jejunum line (IPEC-J2) as an in-vitro model and probiotic treatment. Cell-culture-adapted (PRV) OSU strain, or human (HRV) Wa along with Lactobacillus acidophilus (LA) rhamnosus GG (LGG) were used inoculate IPEC-J2 cells. LA LGG treatment was applied pre- post-rotavirus infection. We demonstrated that cells productively...
Significance An estimated 20 million hepatitis E virus (HEV) infections occur yearly worldwide, leading to 56,600 deaths. Chronic HEV infection has recently become a significant clinical problem in immunocompromised individuals such as organ transplant patients. The lack of an animal model greatly hinders our ability study chronic and develop therapeutics. Here we report the successful development pig by mimicking conditions We demonstrate that active suppression HEV-specific cell-mediated...
ABSTRACT Since May 2013, outbreaks of porcine epidemic diarrhea have devastated the U.S. swine industry, causing immense economic losses. Two different enteric coronaviruses (porcine virus and Delta coronavirus) been isolated from affected population. The disease has reported at least 32 states United States other countries, including Mexico, Peru, Dominican Republic, Canada, Columbia, Ecuador, Ukraine, with repeated in previously infected herds. Here we report isolation characterization a...
ABSTRACT Although few simian rotaviruses (RVs) have been isolated, such strains important for basic research and vaccine development. To explore the origins of RVs, complete genome sequences PTRV (G8P[1]), RRV (G3P[3]), TUCH (G3P[24]) were determined. These data allowed genotype constellations each virus to be determined phylogenetic relationships with other nonsimian RVs elucidated. The results indicate that was likely transmitted from a bovine or ruminant into pig-tailed macaques (its host...
ABSTRACT The roles of conserved nucleotides on the stem-loop (SL) structure in intergenic region hepatitis E virus (HEV) genome replication were determined by using Huh7 cells transfected with HEV SL mutant replicons containing reporter genes. One or two nucleotide mutations AGA motif loop significantly reduced replication, and three more abolished replication. Mutations stem subgenome start sequence also inhibited results indicated that both junction play important
Hepatitis E virus (HEV) is an important human pathogen with pigs and other species serving as natural animal reservoirs. Ample evidence documents sporadic cases of hepatitis acquired via consumption undercooked meat. Chronic in immunosuppressed individuals are mostly caused by zoonotic HEV swine origin. We report here the identification genotype 3 from non-liver commercial pork local grocery stores southwest Virginia, association seropositivity to meat healthy young adults at a university...
Syndecans have three highly conserved sites available for heparan sulfate attachment. To determine if all are required normal function, a series of mutated syndecans having two, one, or no chains were expressed in ARH-77 cells. Previously, we demonstrated that expression wild-type syndecan-1 on these myeloma cells mediates cell-matrix and cell-cell adhesion inhibits cell invasion into collagen gels. Here show to optimally mediate each activities, attachment required. Generally, an increasing...
Hepatitis E virus (HEV), a single-stranded positive-sense RNA virus, generally causes self-limiting acute viral hepatitis, although chronic HEV infection has recently become significant clinical problem in immunocompromised individuals, especially solid-organ transplant recipients. Innate immunity, via the type I interferon (IFN) response, plays an important role during initial stages of infection. IFN-stimulated gene 15 (ISG15), IFN-induced ubiquitin-like protein, is known to have...
Hepatitis E virus (HEV) is a single-strand positive-sense RNA in the family Hepeviridae. The disease caused by HEV, hepatitis E, an important public health problem developing countries of Asia and Africa also endemic many industrialized countries, including United States. HEV has been identified from several other animal species addition to humans, pig, chicken, mongoose, deer, rabbit, ferret, bat, fish. Here we report complete genome sequence first strain rabbits Sequence phylogenetic...
Porcine epidemic diarrhea virus (PEDV) poses a serious threat to swine worldwide as evidenced by its recent introduction into the USA and devastating economic impact it caused industry. Commercial vaccines against PEDV are available but their efficacies inadequate. Therefore, with improved efficacy needed effectively control infections. We previously determined immunogenicity of novel dendritic cell (DC)-targeted S1 protein-based subunit vaccine in weaned piglets which antigen was targeted...
ABSTRACT Molecular breeding via DNA shuffling can direct the evolution of viruses with desired traits. By using a positive-strand RNA virus, porcine reproductive and respiratory syndrome virus (PRRSV), as model, rapid attenuation was achieved in this study by viral envelope genes from multiple strains. The GP5 7 genetically divergent PRRSV strains GP5-M 6 different were molecularly bred iteration process, shuffled cloned into backbone DNA-launched infectious clone. Two representative...
Abstract Hepatitis E virus (HEV), rotavirus (RV), and astrovirus (AstV) are important pathogens that transmit through a common fecal-oral route, causing hepatitis (HEV) gastroenteritis (RV AstV) respectively in humans. In this study, we developed evaluated two subunit vaccine candidates consisted of the same protruding or spike protein antigens three viruses formats, fusion into one molecule (fused vaccine) vs. mixture free together (mixed vaccine). Both vaccines were easily made via E. coli...