A5036192991- Viral Infections and Outbreaks Research
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Bacillus and Francisella bacterial research
- Liver Disease and Transplantation
- Zoonotic diseases and public health
- Fire effects on ecosystems
- Disaster Response and Management
- Ethics and Legal Issues in Pediatric Healthcare
- Veterinary medicine and infectious diseases
- Hepatitis B Virus Studies
- Vector-Borne Animal Diseases
- COVID-19 Clinical Research Studies
- Parvovirus B19 Infection Studies
- Animal Disease Management and Epidemiology
- SARS-CoV-2 detection and testing
- Yersinia bacterium, plague, ectoparasites research
- Poxvirus research and outbreaks
- COVID-19 epidemiological studies
- Brucella: diagnosis, epidemiology, treatment
- Virus-based gene therapy research
Centers for Disease Control and Prevention
2019-2025
National Center for Emerging and Zoonotic Infectious Diseases
2020-2025
Yale University
2023
Makerere University
2022
Uganda Virus Research Institute
2020-2022
Uganda Wildlife Authority
2022
Epidemic Intelligence Service
2022
Texas Department of State Health Services
2020
Ministry of Health
2020
Cheetah Conservation Fund
2019
Abstract We identified hepatitis E virus (HEV) in rabbits Virginia, USA. HEV RNA was detected 14 (16%) of 85 serum samples and 13 (15%) fecal samples. Antibodies against were 31 (36%) Sequence analyses showed that from is closely related to genotype 3.
Hepatitis E virus (HEV) is an important human pathogen. In addition to humans, HEV has also been identified in pig, chicken, mongoose, deer, rat, rabbit and fish. There are four recognized two putative genotypes of mammalian HEV. Genotypes 1 2 restricted while 3 4 zoonotic. The recently a distant member genotype 3. Here, we first expressed purified the recombinant capsid protein showed that cross-reacted with antibodies raised against avian, swine Conversely, proteins derived from Since pigs...
Approximately 67% of U.S. households have pets. Limited data are available on SARS-CoV-2 in We assessed infection pets during a COVID-19 household transmission investigation. Pets from with ≥1 person laboratory-confirmed were eligible for inclusion April-May 2020. enrolled 37 dogs and 19 cats 34 households. All oropharyngeal, nasal, rectal swabs tested negative by rRT-PCR; one dog's fur (2%) positive rRT-PCR at the first sampling. Among 47 serological results, eight (17%) (four dogs, four...
This report summarizes the recommendations of Advisory Committee on Immunization Practices (ACIP) for use rVSVΔG-ZEBOV-GP Ebola vaccine (Ervebo) in United States. The contains rice-derived recombinant human serum albumin and live attenuated vesicular stomatitis virus (VSV) which gene encoding glycoprotein VSV was replaced with species Zaire ebolavirus. Persons a history severe allergic reaction (e.g., anaphylaxis) to rice protein should not receive Ervebo. is first only currently licensed by...
ABSTRACT. Rift Valley fever (RVF) is a zoonotic disease of public health and economic importance. Uganda has reported sporadic outbreaks RVF in both humans animals across the country, especially southwestern part “cattle corridor” through an established viral hemorrhagic surveillance system. We report 52 human cases laboratory-confirmed from 2017 to 2020. The case fatality rate was 42%. Among those infected, 92% were males 90% adults (≥ 18 years). Clinical symptoms characterized by (69%),...
Hepatitis E virus (HEV) is an important human pathogen with pigs and other species serving as natural animal reservoirs. Ample evidence documents sporadic cases of hepatitis acquired via consumption undercooked meat. Chronic in immunosuppressed individuals are mostly caused by zoonotic HEV swine origin. We report here the identification genotype 3 from non-liver commercial pork local grocery stores southwest Virginia, association seropositivity to meat healthy young adults at a university...
Abstract To our knowledge, environmental isolation of Burkholderia pseudomallei, the causative agent melioidosis, from continental United States has not been reported. We report a case melioidosis in Texas resident. Genomic analysis indicated that isolate groups with B. pseudomallei isolates patients same region, suggesting possible endemicity to this region.
In June 2019, the Bolivian Ministry of Health reported a cluster cases hemorrhagic fever that started in municipality Caranavi and expanded to La Paz. The cause these was unknown.We obtained samples for next-generation sequencing virus isolation. Human rodent specimens were tested by means virus-specific real-time quantitative reverse-transcriptase-polymerase-chain-reaction assays, sequencing, isolation.Nine identified; four patients with this illness died. etiologic agent identified as...
Lymphocytic choriomeningitis virus (LCMV) infection in immunocompromised hosts can result disseminated disease, meningoencephalitis, and death. Published cases transplant recipients have been traced to transmission from infected donors. We report 2 of serious, non-donor-derived LCMV solid organ recipients. Initial identification was done by using metagenomic next-generation sequencing (mNGS). Subsequent evaluations confirmatory testing involved molecular diagnostics, serology, phylogenetic...
Hepatitis E virus (HEV) is an important but extremely understudied human pathogen. Genotypes 1 and 2 are restricted to humans, whereas genotypes 3 4 zoonotic, infecting both humans pigs. This report describes, for the first time, successful rescue of infectious HEV in vitro vivo from cloned cDNA a genotype (strain TW6196E). The complete genomic sequence TW6196E was determined full-length clone (pHEV-4TW) assembled. Capped RNA transcripts pHEV-4TW were replication competent Huh7 cells...
Hepatitis E virus (HEV) is a single-strand positive-sense RNA in the family Hepeviridae. The disease caused by HEV, hepatitis E, an important public health problem developing countries of Asia and Africa also endemic many industrialized countries, including United States. HEV has been identified from several other animal species addition to humans, pig, chicken, mongoose, deer, rabbit, ferret, bat, fish. Here we report complete genome sequence first strain rabbits Sequence phylogenetic...
Abstract On April 20, 2018, the Kween District Health Office in District, Uganda reported 7 suspected cases of human anthrax. A team from Ministry and partners investigated identified 49 cases, 3 confirmed 46 suspected; no deaths were reported. Multiple exposures handling carcass a cow that had died suddenly significantly associated with cutaneous anthrax, whereas eating meat was gastrointestinal Eating undercooked but boiling for >60 minutes protective. We recommended providing postexposure...
An animal model that fully recapitulates severe COVID-19 presentation in humans has been a top priority since the discovery of SARS-CoV-2 2019. Although multiple models are available for mild to moderate clinical disease, develop disease still needed. Mink experimentally infected with developed acute respiratory as evident by radiological, and histological changes. Virus was detected nasal, oral, rectal, fur swabs. Deep sequencing from oral swabs lung tissue samples showed repeated...
ObjectiveTo highlight challenges associated with Crimean-Congo hemorrhagic fever (CCHF) prevention, detection, and response in Uganda.MethodsWe describe a single confirmed CCHF case Rakai District 2022. We reviewed medical records conducted interviews on the case-patient's history possible exposures. To understand prevention efforts, we key informant (KIIs) district health veterinary officers focus group discussions (FGDs) farmers affected village.ResultsAn 18-year-old resident had onset...
In the United States (U.S.), hantavirus pulmonary syndrome (HPS) and non-HPS infection are nationally notifiable diseases. Criteria for identifying human cases based on clinical symptoms (HPS or non-HPS) acute diagnostic results (IgM+, rising IgG+ titers, RT-PCR+, immunohistochemistry (IHC)+). Here we provide an overview of testing summarize Hantavirus disease occurrence genotype distribution in U.S. from 2008 to 2020.
Genotypes 3 and 4 hepatitis E virus (HEV) strains within the species Orthohepevirus A in family Hepeviridae are zoonotic. Recently, a genotype HEV was reportedly detected fecal samples of cows, although independent confirmation is lacking. In this study, we first tested serum from 983 cows different regions United States for presence immunoglobulin G (IgG) anti-HEV found that 20.4% were seropositive. The highest seroprevalence rate (68.4%) herd Georgia. an attempt to genetically identify...
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important swine pathogens, which causes failure in sows disease piglets. A major hurdle to control PRRSV ineffectiveness current vaccines confer protection against heterologous strains. Since both GP4 M genes induce neutralizing antibodies, this study we molecularly bred through DNA shuffling genes, separately, from six genetically different strains an attempt identify chimeras with improved...
On December 19, 2019, the Food and Drug Administration (FDA) approved rVSVΔG-ZEBOV-GP Ebola vaccine (ERVEBO, Merck) for prevention of virus disease (EVD) caused by infection with virus, species Zaire ebolavirus, in adults aged ≥18 years. In February 2020, Advisory Committee on Immunization Practices (ACIP) recommended preexposure vaccination ERVEBO years United States who are at highest risk potential occupational exposure to because they responding an outbreak EVD, work as health care...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes disease in mink similar to human COVID-19. We characterized the pathological findings 72 from US farms with SARS-CoV-2 outbreaks, localized and its host cellular receptor angiotensin-converting enzyme (ACE2) tissues, evaluated utility of various test methods specimens for detection necropsy tissues. Of SARS-CoV-2-positive animals found dead, 74% had bronchiolitis diffuse alveolar damage (DAD). euthanized animals, 72% only...
Background In September 2022, Uganda experienced an outbreak of Sudan virus disease (SVD), mainly in central Uganda. As a result enhanced surveillance activities for Ebola disease, samples from several patients with suspected viral hemorrhagic fever (VHF) were sent to the VHF Program at Virus Research Institute (UVRI), Entebbe, Uganda, and identified infections caused by other etiologies. Herein, we report epidemiologic laboratory findings Crimean-Congo (CCHF) cases that detected during SVD...