James Graziano
A5017400935- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Fire effects on ecosystems
- COVID-19 epidemiological studies
- Hepatitis B Virus Studies
- Vector-Borne Animal Diseases
- Disaster Response and Management
- Viral gastroenteritis research and epidemiology
- Actinomycetales infections and treatment
- Streptococcal Infections and Treatments
- Infections and bacterial resistance
- Bat Biology and Ecology Studies
- Mosquito-borne diseases and control
- Microbial Applications in Construction Materials
- Genomics and Phylogenetic Studies
- Catalysis and Hydrodesulfurization Studies
- Fungal Infections and Studies
- Infective Endocarditis Diagnosis and Management
- Biodiesel Production and Applications
- Antimicrobial Resistance in Staphylococcus
- Plant Disease Resistance and Genetics
- Rabies epidemiology and control
- Autoimmune and Inflammatory Disorders Research
- Oral microbiology and periodontitis research
- Virology and Viral Diseases
Centers for Disease Control and Prevention
2012-2025
National Center for Emerging and Zoonotic Infectious Diseases
2020-2025
Jordan University of Science and Technology
2021
Southampton Hospital
2019
New York Hospital Queens
2019
Presbyterian Hospital
2019
NewYork–Presbyterian Hospital
2019
Cornell University
2019
Stony Brook University
2019
Icahn School of Medicine at Mount Sinai
2019
Compared with the volume of data on coronavirus disease 2019 (COVID-19) outbreaks among older adults, relatively few are available concerning COVID-19 in younger, healthy persons United States (1,2). In late March 2020, aircraft carrier USS Theodore Roosevelt arrived at port Guam after numerous U.S. service members onboard developed COVID-19. April, Navy and CDC investigated this outbreak, demographic, epidemiologic, laboratory findings a convenience sample 382 serving aboard reported study....
Abstract Marburg virus (MARV) causes sporadic outbreaks of severe disease (MVD). Most MVD originated in East Africa and field studies Africa, South Zambia, Gabon identified the Egyptian rousette bat (ERB; Rousettus aegyptiacus ) as a natural reservoir. However, largest recorded outbreak with highest case–fatality ratio happened 2005 Angola, where direct spillover from bats was not shown. Here, collaborative by Centers for Disease Control Prevention, Njala University, University California,...
Bats are increasingly recognized as reservoirs of emerging zoonotic pathogens. Egyptian rousette bats (ERBs) the known reservoir Marburg virus (MARV), a filovirus that causes deadly disease (MVD) in humans. However, ERBs harbor MARV asymptomatically, likely due to coadapted and specific host immunity-pathogen relationship. Recently, we measured transcriptional responses MARV-infected ERB whole tissues, showing these possess tolerant strategy limits pro-inflammatory gene induction, presumably...
Introduction In October 2017, a blood sample from resident of Kween District, Eastern Uganda, tested positive for Marburg virus. Within 24 hour confirmation, rapid outbreak response was initiated. Here, we present results epidemiological and laboratory investigations. Methods A district task force activated consisting specialised teams to conduct case finding, management isolation, contact listing follow up, collection testing, community engagement. An ecological investigation also carried...
ABSTRACT. Rift Valley fever (RVF) is a zoonotic disease of public health and economic importance. Uganda has reported sporadic outbreaks RVF in both humans animals across the country, especially southwestern part “cattle corridor” through an established viral hemorrhagic surveillance system. We report 52 human cases laboratory-confirmed from 2017 to 2020. The case fatality rate was 42%. Among those infected, 92% were males 90% adults (≥ 18 years). Clinical symptoms characterized by (69%),...
In June 2019, the Bolivian Ministry of Health reported a cluster cases hemorrhagic fever that started in municipality Caranavi and expanded to La Paz. The cause these was unknown.We obtained samples for next-generation sequencing virus isolation. Human rodent specimens were tested by means virus-specific real-time quantitative reverse-transcriptase-polymerase-chain-reaction assays, sequencing, isolation.Nine identified; four patients with this illness died. etiologic agent identified as...
Sosuga virus (SOSV), a rare human pathogenic paramyxovirus, was first discovered in 2012 when person became ill after working South Sudan and Uganda. During an ecological investigation, several species of bats were sampled tested for SOSV RNA only one species, the Egyptian rousette bat (ERBs;
The Egyptian rousette bat (ERB) is a natural reservoir for Marburg virus (MARV; family Filoviridae ), putative Sosuga (SOSV; Paramyxoviridae and vertebrate Kasokero (KASV; Orthonairoviridae ); however, the effect of naturally occurring coinfection by those viruses on MARV shedding spillover potential unknown. To answer this question, we experimentally infected one cohort captive-bred ERBs with SOSV+MARV (n=12 bats) or only second KASV+MARV bats), then collected blood, oral swab, rectal swab...
ABSTRACT Marburg virus (MARV) and Ravn (RAVV), the only two known members of species Orthomarburgvirus marburgense (family Filoviridae ), are causative agents disease, a severe viral disease that typically emerges in sub-Saharan Africa is characterized by human-to-human transmission high case fatalities. Despite robust characterization MARV experimental infection Egyptian rousette bats (ERBs; Rousettus aegyptiacus ; common name: rousettes), natural reservoir, with RAVV ERBs has not been...
We report 1.3% (19/1,511) of Egyptian rousette bats (ERBs) in Uganda and Sierra Leone were co-infected with different combinations Marburg, Sosuga, Kasokero, or Yogue viruses. To prevent infection by those viruses, we recommend avoiding ERB-populated areas, ERBs ERB-contaminated objects, thoroughly washing harvested fruits before consumption.
In December 2016, the Wisconsin Department of Health Services (WDHS) notified CDC a patient hospitalized with fever, leukopenia, elevated transaminases, and proteinuria. The owned operated an in-home rattery, or rat-breeding facility, approximately 100 Norway rats, primarily bred as pets. A family member developed similar symptoms 4 weeks later, but was not hospitalized. Because both patients were known to have rodent contact, they tested for hantavirus infections. January 2017, confirmed...
Abstract Background During 2017, a multistate outbreak investigation occurred after the confirmation of Seoul virus (SEOV) infections in people and pet rats. A total 147 humans 897 rats were tested. Methods In addition to immunoglobulin (Ig)G IgM serology traditional reverse-transcription polymerase chain reaction (RT-PCR), novel quantitative RT-PCR primers/probe developed, whole genome sequencing was performed. Results Seventeen had SEOV IgM, indicating recent infection; 7 reported symptoms...
During the Ebola virus outbreak of 2013–2016, Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis B2M endogenous control Ct values showed its utility monitoring specimen quality, comparing results with different types, interpretation results. For live patients, blood is most sensitive type oral swabs have little diagnostic utility. However, are highly for testing corpses.
Egyptian rousette bats (ERBs; Rousettus aegyptiacus; family Pteropodidae) are associated with a growing number of bunyaviruses public health importance, including Kasokero virus (KASV), which was first identified as zoonosis in Uganda 1977. In this study, formalin-fixed paraffin-embedded tissues from previous experiment KASV infection confirmed 18 experimentally infected ERBs were used for an in-depth analysis using histopathology, situ hybridization (ISH) detection viral RNA,...
ABSTRACT Ninety-seven animal, human, and dairy Streptococcus porcinus or pseudoporcinus isolates in the CDC strain collection were evaluated on basis of DNA-DNA reassociation, 16S rRNA rpoB gene sequencing, conventional biochemical Rapid ID 32 Strep identification methods, antimicrobial susceptibility testing to determine their taxonomic status, characteristics for species differentiation, susceptibility, relevance clinical source. Nineteen 97 (1 18 swine) identified as S. . The remaining 72...
Marburg virus disease, caused by and Ravn orthomarburgviruses, emerges sporadically in sub-Saharan Africa is often fatal humans. The natural reservoir the Egyptian rousette bat (ERB), which sheds saliva, urine, feces. Frugivorous ERBs discard test-bitten partially eaten fruit, potentially leaving infectious behind that could be consumed other susceptible animals or Historically, 8 of 17 known disease outbreaks have been linked to human encroachment on ERB habitats, but no linkage exists for...
Abstract The human-pathogenic Kasokero virus (KASV; genus Orthonairovirus ) has been isolated from the sera of Egyptian rousette bats (ERBs; Rousettus aegyptiacus captured in Uganda and unengorged Ornithodoros (Reticulinasus) faini ticks collected rock crevices ERB colonies South Africa Uganda. Although evidence suggests that KASV is maintained an enzootic transmission cycle between O. (R.) ERBs with potential for incidental spillover to humans through bite infected tick, vertebrate...
Draft genome sequences of Elizabethkingia meningoseptica and representatives each its four historically described genomospecies were sequenced here. Preliminary analysis suggests that miricola belongs to 2, both anophelis endophytica are most similar 1.
The Democratic Republic of the Congo (DRC) declared an Ebola virus disease (EVD) outbreak in North Kivu August 2018. By June 2019, had spread to 26 health zones northeastern DRC, causing >2,000 reported cases and >1,000 deaths. On 10, three members a Congolese family with EVD-like symptoms traveled western Uganda’s Kasese District seek medical care. Shortly thereafter, Viral Hemorrhagic Fever Surveillance Laboratory Program (VHF program) at Uganda Virus Research Institute (UVRI)...
Abstract Background Long-term persistence of Ebola virus (EBOV) in immunologically privileged sites has been implicated recent outbreaks disease (EVD) Guinea and the Democratic Republic Congo. This study was designed to understand how acute course EVD, convalescence, host immune genetic factors may play a role prolonged viral semen. Methods A cohort 131 male EVD survivors Liberia were enrolled case-case study. “Early clearers” defined as those with 2 consecutive negative EBOV semen test...
Abstract Seoul orthohantavirus (SEOV) infections, uncommonly reported in the United States, often result mild illness. We report a case of hemophagocytic lymphohistiocytosis secondary to SEOV infection that was domestically acquired Washington, DC.
Ebola virus (EBOV), species Zaire ebolavirus, may persist in the semen of male survivors disease (EVD). We conducted a study 2014-2016 EVD outbreak Liberia and evaluated their immune responses to EBOV. report here findings from serologic testing blood for EBOV-specific antibodies, molecular EBOV semen, peripheral mononuclear cells (PBMCs) subset participants.We tested RNA by quantitative reverse transcription polymerase chain reaction (qRT-PCR), anti-EBOV-specific immunoglobulin M (IgM) G...
Streptomyces cacaoi subsp. cacaoi, a Gram-positive, branching filamentous bacteria, was isolated from scalp infection in patient Pondicherry, India. Phenotypic tests identified the isolate as species, but 16S rRNA sequence analysis provided species identification required for tracking of this emerging pathogen.