A5068712912- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Fire effects on ecosystems
- Disaster Response and Management
- Hepatitis B Virus Studies
- Zoonotic diseases and public health
- interferon and immune responses
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- SARS-CoV-2 and COVID-19 Research
Public Health Agency of Canada
2012-2022
Centers for Disease Control and Prevention
2015-2020
National Center for Emerging and Zoonotic Infectious Diseases
2015-2020
National Institutes of Health
2011-2018
University of Manitoba
2011-2018
National Institute of Allergy and Infectious Diseases
2011-2018
Global Viral
2015-2016
State Key Laboratory of Virology
2013
Crimean-Congo hemorrhagic fever (CCHF) is a widely distributed viral characterized by rapid onset of flu-like symptoms often followed manifestations. CCHF virus (CCHFV), bunyavirus in the Nairovirus genus, capable infecting wide range mammalian hosts nature but so far only causes disease humans. Recently, immunocompromised mice have been reported as models, detailed characterization lacking. Here, we closely infection and progression CCHFV-infected interferon α/β receptor knockout (IFNAR−/−)...
Ebola hemorrhagic fever (EHF) is a severe viral infection for which no effective treatment or vaccine currently available. While the nonhuman primate (NHP) model used final evaluation of experimental vaccines and therapeutic efficacy, rodent models have been widely in ebolavirus research because their convenience. However, validity has questioned given low predictive value efficacy testing therapeutics, result inconsistent manifestation coagulopathy seen EHF. Here, we describe lethal Syrian...
Crimean-Congo hemorrhagic fever virus (CCHFV) is a tick-borne capable of causing severe disease in humans. There are currently no licensed vaccines to prevent CCHFV-associated disease. We developed DNA vaccine expressing the M-segment glycoprotein precursor gene CCHFV and assessed its immunogenicity protective efficacy two lethal mouse models disease: type I interferon receptor knockout (IFNAR-/-) mice; novel transiently immune suppressed (IS) model. Vaccination mice by muscle...
Crimean Congo hemorrhagic fever virus (CCHFV) is a negative-strand RNA of the family Bunyaviridae (genus: Nairovirus). In humans, CCHFV causes fever, hemorrhage, severe thrombocytopenia, and high fatality. A major impediment in precisely determining basis CCHFV's pathogenicity has been lack methodology to produce recombinant CCHFV. We developed reverse genetics system based on transfecting plasmids into BSR-T7/5 Huh7 cells. our system, bacteriophage T7 polymerase produced complementary...
Crimean-Congo hemorrhagic fever (CCHF) is an acute, often fatal viral disease characterized by rapid onset of febrile symptoms followed manifestations. The etiologic agent, CCHF orthonairovirus (CCHFV), can infect several mammals in nature but only seems to cause clinical humans. Over the past two decades there has been increase total number case reports, including imported patients, and expansion endemic areas. Despite its increased public health burden are currently no licensed vaccines or...
Hantavirus pulmonary syndrome (HPS), also referred to as hantavirus cardiopulmonary (HCPS), is a rare but frequently fatal disease caused by New World hantaviruses. In humans HPS associated with severe edema and cardiogenic shock; however, the pathogenesis of this remains unclear largely due lack suitable animal models for study progression. we monitored clinical, virological, pathophysiological parameters host immunological responses decipher pathological factors events in lethal Syrian...
Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral disease seen exclusively in humans. Central nervous system (CNS) infection and neurological involvement have also been reported CCHF. In the current study, we inoculated NSG-SGM3 mice engrafted with human hematopoietic CD34+ stem cells low-passage CCHF virus strains isolated from patients. humanized mice, lethal develops, characterized by histopathological change liver brain. To date, targets of not investigated CNS was gliosis,...
Crimean-Congo hemorrhagic fever (CCHF) is an often lethal, acute inflammatory illness that affects a large geographic area. The disease caused by infection with CCHF virus (CCHFV), nairovirus from the Bunyaviridae family. Basic research on CCHFV has been severely hampered biosafety requirements and lack of available strains molecular tools. We report development transcription- entry-competent virus-like particle (tecVLP) system can be used to study cell entry viral transcription/replication...
Background Lassa fever is an acute viral illness characterized by multi-organ failure and hemorrhagic manifestations. most frequently diagnosed in Nigeria, Sierra Leone, Liberia, Guinea, although sporadic cases have been recorded other West African countries, including Mali. The etiological agent of virus (LASV), Arenavirus which maintained nature transmitted to humans Mastomys natalensis. purpose this study was better define the geographic distribution LASV-infected rodents sub-Saharan...
In the cytoplasm, retinoic acid-inducible gene I (RIG-I) senses RNA genomes of several viruses. RIG-I binds to viral RNA, eliciting an antiviral response via cellular adaptor MAVS. Crimean-Congo hemorrhagic fever virus (CCHFV), a negative-sense with 5'-monophosphorylated genome, is highly pathogenic zoonotic agent significant public health implications. We found that, during CCHFV infection, mediated type interferon (IFN) Interfering signaling reduced IFN production and IFN-stimulated...
In August 2014, the Viral Special Pathogens Branch of US Centers for Disease Control and Prevention established a field laboratory in Sierra Leone response to ongoing Ebola virus outbreak. Through March 2015, this tested >12 000 specimens from throughout Leone. We describe organization procedures located Bo,
During the Ebola virus outbreak of 2013–2016, Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis B2M endogenous control Ct values showed its utility monitoring specimen quality, comparing results with different types, interpretation results. For live patients, blood is most sensitive type oral swabs have little diagnostic utility. However, are highly for testing corpses.
ABSTRACT We report here the full-length sequences of 16 historical isolates Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV) obtained in Turkey, Namibia, and South Africa. The strains may be useful for future work to develop molecular diagnostics or viral evolution studies.
Abstract The COVID-19 pandemic required increased testing capacity, enabling rapid case identification and effective contract tracing to reduce transmission of disease. BioFire FilmArray is a fully automated nucleic acid amplification test system providing specificity sensitivity associated with gold standard molecular methods. Respiratory Panel 2.1 targets 22 viral bacterial pathogens, including SARS-CoV-2 influenza virus. While each panel provides robust output information regarding...