A5100701204- Hippo pathway signaling and YAP/TAZ
- Autophagy in Disease and Therapy
- Angiogenesis and VEGF in Cancer
- Mycobacterium research and diagnosis
- Cellular Mechanics and Interactions
- Lipid metabolism and disorders
- Cancer, Hypoxia, and Metabolism
- Protease and Inhibitor Mechanisms
- Infectious Diseases and Tuberculosis
- Tuberculosis Research and Epidemiology
- Peptidase Inhibition and Analysis
- Axon Guidance and Neuronal Signaling
- Neurofibromatosis and Schwannoma Cases
- Medicinal Plants and Bioactive Compounds
- Wnt/β-catenin signaling in development and cancer
- Phytochemical compounds biological activities
- Cancer Research and Treatments
- Cancer Mechanisms and Therapy
- Advanced Glycation End Products research
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Cassava research and cyanide
- Cell death mechanisms and regulation
- Actinomycetales infections and treatment
- Pancreatic function and diabetes
Peking University
2020-2024
Jiangsu Provincial Center for Disease Control and Prevention
2023
Xinxiang Medical University
2023
Shandong Provincial Hospital
2023
Icahn School of Medicine at Mount Sinai
2008-2021
Huazhong University of Science and Technology
2015-2020
Tongji Hospital
2015-2020
Fourth Affiliated Hospital of China Medical University
2020
China Medical University
2020
University of California, Los Angeles
2013-2017
Treatment options for late stage prostate and colon cancer are limited there is an urgent need to develop more effective targeted novel therapies, which starts with identification validation of therapeutic targets. Recent clinical studies have demonstrated that tissue inhibitor matrix metalloproteinase-1 (TIMP-1) levels elevated in patient plasma TIMP-1 associated worse outcomes. However, it unknown whether serves merely as a biomarker progression or has functional role promoting can serve...
Currently, targeting the autophagic pathway is regarded as a promising new strategy for cancer drug discovery. Here, we screened North China Pharmaceutical Group Corporation's pure compound library of microbial origin using GFP-LC3B-SKOV3 cells and identified elaiophylin novel autophagy inhibitor. Elaiophylin promotes autophagosome accumulation but blocks flux by attenuating lysosomal cathepsin activity, resulting in SQSTM1/p62 various cell lines. Moreover, destabilizes lysosomes indicated...
Abstract Neurofibromatosis 2 (NF2) is an inherited cancer syndrome in which affected individuals develop nervous system tumors, including schwannomas, meningiomas, and ependymomas. The NF2 protein merlin (or schwannomin) a member of the Band 4.1 superfamily proteins, serve as linkers between transmembrane proteins actin cytoskeleton. In addition to mutational inactivation gene NF2-associated mutations loss expression have also been reported other types cancers. present study, we show that...
Significance Autophagy is known to promote cell survival through providing various sources of fuels as a result digestion. Here, we uncover function autophagy in metabolic regulation by directly activating cAMP/PKA signaling that mitochondrial metabolism and confers resistance energy crisis. We find selectively degrades PKA inhibitory subunit RIα new receptor AKAP11 response crisis, thus causing activation, which central hub for variety cellular signaling. Our study suggests tumor cells may...
Glioblastoma multiforme (GBM) is a highly invasive and vascularized aggressive brain tumor. Less than 10% of GBM patients survive >5 years after diagnosis. Angiogenesis plays an important role in growth, antiangiogenesis-based therapies have shown clinical efficacy for patients. Unfortunately, therapeutic resistance often develops these patients, suggesting that cells are capable switching their dependency on one proangiogenic signaling pathway to alternative one. Therefore, it identify...
Ovarian carcinoma is the most deadly gynecological malignancy. Current chemotherapeutic drugs are only transiently effective and patients with advance disease often develop resistance despite significant initial responses. Mounting evidence suggests that anti-apoptotic proteins, including those of inhibitor apoptosis protein (IAP) family, play important roles in chemoresistance. There has been a recent emergence compounds block IAP functions. Here, we evaluated AT-406, novel orally active...
Despite decades of research, the survival rate ovarian cancer patients is largely unchanged. Current chemotherapeutic drugs are effective only transiently because with advanced disease eventually develop resistance. Thus, there a pressing need for identifying novel therapeutic targets in cancer. Mounting evidence suggests that angiopoietins (Angpts) may play an essential role progression; however, expression profiles and biological effects Angpts on remain unknown. Here, we show that,...
: (G. lucidum, Lingzhi) is a medicinal and edible homologous traditional Chinese medicine that used to treat various diseases, including Alzheimer's disease mood disorders. We previously reported the sporoderm-removed
ABSTRACT Prostate cancer (PCa) is one of the malignant tumors affecting men and an important reason for increase in male mortality worldwide. The pathogenesis PCa not fully understood. Thus, there urgent need to discover novel therapeutic targets facilitate development effective anti‐PCa strategies. Quantitative real‐time PCR Western blot were applied detect PODXL2 expressions tissues cells. Progression‐free survival patients was assessed using Kaplan–Meier analysis. relevance between...
Merlin is encoded by the neurofibromatosis type 2 (NF2) gene and a member of Band 4.1 protein family. This acts as linker that connects cell surface proteins to actin cytoskeleton. Defects caused mutations NF2 give rise disease, which generally characterized formation bilateral vestibular schwannomas and, lesser extent, meningiomas ependymomas. In addition these tumor types, mutated and/or merlin expression reduced or lost in numerous non-NF2 associated tumors, including melanoma. However,...
FRMD6 is an Ezrin/Radixin/Moesin (ERM) family protein and a human homologue of Drosophila expanded (ex). Ex functions in parallel merlin at upstream the Hippo signaling pathway that controls proliferation, apoptosis, tissue regeneration, tumorigenesis. Even though core kinase cascade (MST1/2-Lats1/2-YAP/TAZ) has been well established, its regulators are not understood. Merlin promotes activation pathway. However, effect on controversial. Little known about how potential role FRMD...
Pulmonary diseases caused by nontuberculous mycobacteria (NTM) are increasing in incidence and prevalence worldwide. In this study, we identified NTM species of the clinical isolates from 8 provinces China, order to preliminarily provide some basic scientific data different distribution related pulmonary disease China. A total 523 patients with tuberculosis (TB) diagnosed clinically 2005 2012 were using conventional molecular methods, including multilocus PCR, rpoB hsp65 PCR-PRA, hsp65,...
Ganoderma lucidum (G. lucidum, Lingzhi), also known as "immortality mushroom" has been broadly used to improve health and longevity for thousands of years in Asia. G. its spores have promote health, based on broad pharmacological therapeutic activity. This species is recorded Chinese traditional formula a nootropic suggested cognitive dysfunction Alzheimer's disease. However, little about the effects molecular mechanism action spores. The present study investigated protective...
To investigate the role of AEG-1 in glycolysis and tumorigenesis, we construct myc-AEG-1 expression vector demonstrate a novel mechanism that may increase activity AMPK by Thr172 phosphorylation. The higher levels colorectal carcinoma cells were found but showed significant difference different cell lines. study cells, was constructed transfected into NCM460 colonic epithelial cells. We observed consistent increasing glucose consumption lactate production, typical features anaerobic...
Autophagy is a major intracellular degradation system that plays an important role in several biological processes. Although some studies indicate autophagy may play lens and cataracts formation, its underlying mechanism remains to be elucidated. Autophagy‑related gene 4a (ATG4a) cleaves autophagy‑related protein 8 (Atg8) near the C terminus, allowing Atg8 conjugate with phosphatidylethanolamine via exposed glycine; although this pivotal cancer development, no study has yet linked it eye...
Objective A retrospective study comparing the diagnostic performance of BACTEC™ MGIT™ 960 system (M960 system; BD Worldwide, Franklin Lakes, NJ, USA) with Löwenstein–Jensen (L–J) culture to detect mycobacteria in sputum specimens from patients suspected pulmonary tuberculosis (TB). Methods Sputum samples were analysed for presence acid-fast bacilli (AFB). Samples inoculated into M960 and L–J culture. Positive cultures examined AFB. Results The method detected significantly more positive than...
Abstract Merlin is a cytoskeletal linker protein encoded by the neurofibromatosis type 2 (NF2) gene. Like other Band 4.1 family members, it serves to connect cell surface proteins actin cytoskeleton. Defects in caused mutations of NF2 gene give rise disease, which generally characterized formation bilateral vestibular schwannomas and, lesser extent, meningiomas and ependymomas. In addition these tumor types, merlin has been shown be mutated and/or expression lost cancers including...
As severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to disperse globally with worrisome speed, identifying amino acid variations in the virus could help understand characteristics of it. Here, we studied 489 SARS-CoV-2 genomes obtained from 32 countries Nextstrain database and performed phylogenetic tree analysis by clade, country, genotype surface spike glycoprotein (S protein) at site 614. We found that strains mainland China were mostly distributed Clade B undefined...
Objective To compare the diagnostic performance of manual BACTEC™ Mycobacteria Growth Indicator Tube (MGIT™) system (M-MGIT) with automated MGIT™ 960 (A-MGIT) and Löwenstein-Jensen (L-J) culture method in detecting mycobacteria sputum specimens from patients suspected pulmonary tuberculosis (TB). Methods For this cross-sectional study, samples were taken aged ≥18 years attending a TB clinic Beijing, China between July 2015 October 2016. Processed inoculated into MGIT systems L-J medium for...
Abstract Despite advances in surgical debulking, radiation, and chemotherapy over the past few decades, ovarian carcinoma remains most deadly gynecological malignancy. This is largely due to lack of early stage detection manifesting approximately 80% patients presenting with metastatic disease at time diagnosis. Current chemotherapeutic drugs are effective only for a short period as advance eventually develop resistance despite significant initial responses. Thus, there pressing need...