A5032617603- Connexins and lens biology
- Electromagnetic Fields and Biological Effects
- Heat shock proteins research
- Intraocular Surgery and Lenses
- Cancer-related molecular mechanisms research
- Corneal surgery and disorders
- Ophthalmology and Visual Impairment Studies
- Redox biology and oxidative stress
- Yersinia bacterium, plague, ectoparasites research
- Glaucoma and retinal disorders
- Heme Oxygenase-1 and Carbon Monoxide
- MicroRNA in disease regulation
- Ion-surface interactions and analysis
- Human Health and Disease
- Mitochondrial Function and Pathology
- Retinal Diseases and Treatments
- Circular RNAs in diseases
- Cardiomyopathy and Myosin Studies
- Glutathione Transferases and Polymorphisms
- Semiconductor materials and devices
- Retinal and Macular Surgery
- Thermal Radiation and Cooling Technologies
- COVID-19 and Mental Health
- Thin-Film Transistor Technologies
- Cell death mechanisms and regulation
Second Affiliated Hospital of Zhejiang University
2010-2025
Shanghai Advanced Research Institute
2025
Chinese Academy of Sciences
2025
Eye Center
2008-2024
First Affiliated Hospital of Harbin Medical University
2024
Harbin Medical University
2024
Zhejiang University
2007-2023
Ningbo First Hospital
2017-2020
Wenzhou Medical University
2018
Affiliated Eye Hospital of Wenzhou Medical College
2018
Importance After cataract surgery, postoperative residual astigmatism can influence a patient’s visual quality and satisfaction. Finding ways to minimize this is important. Objective To compare the clinical outcomes of femtosecond laser arcuate keratotomy (FSAK) toric intraocular lens (TIOL) implantation for correction in patients undergoing laser-assisted surgery. Design, Setting, Participants This randomized trial was conducted between October 2021 September 2023 at Eye Center Second...
ABSTRACT This study investigated a Chinese family with congenital posterior polar cataracts linked to the βB2‐R188C mutation. βB2‐crystallin, key structural component of lens, is crucial for maintaining lens transparency and stability. We examined effects R188C mutation on βB2‐crystallin's stability resistance environmental stressors using purified proteins cellular models. The mutant showed poor tendency aggregate under physiological pathological conditions. disrupted oligomerization...
Objectives The aims of the present study were to determine oxidative stress and explore possible reasons reactive oxygen species (ROS) increase in human lens epithelial (HLE) B3 cells exposed low intensity 1.8 GHz radiofrequency fields (RF). Methods HLE divided into RF exposure sham-exposure groups. was at specific absorption rate (SAR) 2, 3, or 4 W/kg. ROS levels measured by a fluorescent probe 2′7′-dichlorofluorescin diacetate (DCFH-DA) assay for 0.5, 1, 1.5 h. Lipid peroxidation cellular...
Oxidative stress plays an essential role in the development of age-related cataract. Thioredoxin binding protein-2 (TBP-2) is a negative regulator thioredoxin (Trx), which deteriorates cellular antioxidant system. Our study focused on autophagy-regulating effect TBP-2 under oxidative human lens epithelial cells (LECs). Human were used for cell culture and treatment. Lentiviral-based transfection system was overexpression TBP-2. Cytotoxicity assay, western blot analysis, GFP/mCherry-fused LC3...
To investigate the influence of 1.8-GHz radiofrequency fields (RFs) Global System for Mobile Communications on DNA damage, intracellular reactive oxygen species (ROS) formation, cell cycle, and apoptosis in human lens epithelial cells (hLECs) whether effects induced by RF could be blocked superposing electromagnetic noise.After 24-hour intermittent exposure at specific absorption rate 1 W/kg, 2 3 4 damage hLECs was examined alkaline comet assay immunofluorescence microscope detection...
To assess the safety of femtosecond laser-assisted cataract surgery (FLACS) based on surgical parameters and intraoperative complications analysis to determine length learning curve for FLACS.
To compare the clinical outcomes of echelette extended range vision (ERV) and diffractive bifocal intraocular lenses (IOLs). This is a prospective, consecutive, nonrandomized trial. Seventy-three eligible patients (109 eyes) received implantation ERV IOL (Tecnis Symfony ZXR00) or ZMB00). 1 week, month, 3 months after surgery, visual acuities at different distances were examined. At months, defocus curves, contrast sensitivities (CSs) with without glare, optic path difference (OPD) scans,...
Necroptosis is a new type of cell death. However, the role necroptosis in LPS-related cardiomyocyte damage has not been fully understood. The aim our study to explore molecular mechanism underlying inflammation-mediated necroptosis. H9C2 line was treated with LPS. Then, viability and were measured through qPCR ELISA. Pathway analysis performed verify whether Ripk3/Pgam5 signaling pathway implicated into regulation results demonstrated that LPS reduced activated At levels, oxidative stress...
Inherited mitochondrial mutations can result in dysfunction or stochastic oxidative damage. Cumulative damage is an important factor age-related disorders, such as cataracts and macular degeneration. Mfn2 mediates the fusion of mitochondria contribute to dynamic balance between fission that determines morphology. We report here conditional loss function head surface ectoderm leads a range congenital eye defects, including small, opacified lens small eyeball most severe phenotypes. The Le-Cre...
<h3>Aim</h3> To compare lens extraction (LE) and laser peripheral iridotomy (LPI) on anterior segment morphology in primary angle-closure suspect (PACS) eyes. <h3>Methods</h3> This prospective clinical trial included 144 patients with PACS who underwent either LPI or LE. Ultrasound biomicroscopy (UBM) gonioscopy were performed before 1 month 2 years after operation. Main outcomes UBM parameters the percentage of residual angle closure. <h3>Results</h3> At both follow-up, LE showed a better...
Oxidative stress plays a major role in age-related cataract development. The cellular antioxidant protein thioredoxin-1 (Trx-1) and its negative regulator, thioredoxin binding protein-2 (TBP-2), are pivotal the redox balance during oxidative stress. aim of this study is to investigate effect Trx-1 TBP-2 on LC3 I/LC3 II stress-induced autophagy human lens epithelial cells (LECs). In our study, LECs were treated with 50 μM H2 O2 for different durations, expression measured by RT-PCR Western...
Cataract, opacity of the eye lens, is leading cause visual impairment worldwide. The crucial pathogenic factors that cataract are misfolding and aggregation crystallin protein. βB1-crystallin, which most abundant water-soluble protein in mammalian essential for lens transparency. A previous study identified missense mutation βB1-S93R being responsible congenital cataract. However, exact mechanism causing remains unclear. S93 residue, located at first Greek-key motif highly conserved, its...
Objective: The aim of the present study was to observe effects 1.8 GHz radiofrequency (RF) radiation on protein expression human lens epithelial cells (hLECs) in vitro. Methods: hLECs were exposed and sham-exposed RF (specific absorption rate (SAR) 4 W/kg) for 2 h. After exposure, proteins extracted from LECs loaded Ettan MDLC system connected LTQ-Orbitrap MS screening candidate biomarkers induced by RF. quantitative real-time polymerase chain reaction (qRT-PCR) used detect levels messenger...