A5071399347- RNA modifications and cancer
- Medical Imaging and Pathology Studies
- Reproductive tract infections research
- Advanced biosensing and bioanalysis techniques
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Retinal and Optic Conditions
- Ocular Diseases and Behçet’s Syndrome
- Retinal Development and Disorders
- Ubiquitin and proteasome pathways
- Corneal Surgery and Treatments
- Advancements in PLL and VCO Technologies
- Radio Frequency Integrated Circuit Design
- Retinal Diseases and Treatments
- Semiconductor materials and devices
- Reproductive System and Pregnancy
- Phagocytosis and Immune Regulation
- Corneal surgery and disorders
- Immunotherapy and Immune Responses
- Neurofibromatosis and Schwannoma Cases
- Systemic Lupus Erythematosus Research
- Epigenetics and DNA Methylation
- Nutrition, Genetics, and Disease
- Autophagy in Disease and Therapy
University of California, Los Angeles
2011-2024
Doheny Eye Institute
2013-2024
University of Michigan–Ann Arbor
2020
UCLA Health
2020
Hong Kong Polytechnic University
2018
Berkeley College
2018
Southern California Eye Institute
2017
Hong Kong University of Science and Technology
2002
University of Hong Kong
2002
Rambus (United States)
1993
A modification of stacked spiral inductors increases the self-resonance frequency by 100% with no additional processing steps, yielding values 5 to 266 nH and frequencies 11.2 0.5 GHz. Closed-form expressions predicting less than 5% error have also been developed. Stacked transformers are introduced that achieve voltage gains 1.8 3 at multigigahertz frequencies. The structures fabricated in standard digital CMOS technologies four five metal layers.
Mesenchymal stem cells (MSCs) are being tested in a wide range of human diseases; however, loss potency and inconsistent quality severely limit their use. To overcome these issues, we have utilized developmental precursor called the hemangioblast as an intermediate cell type derivation highly potent replenishable population MSCs from embryonic (hESCs). This method circumvents need for labor-intensive hand-picking, scraping, sorting that other hESC-MSC methods require. Moreover, unlike...
Generation of robust cell-mediated immune responses at mucosal surfaces while reducing overall inflammation is a primary goal for vaccination. Here we report the use recombinant nanoparticle as vaccine delivery platform against infections requiring T immunity eradication.We encapsulated an immunogenic protein, major outer membrane protein (MOMP) Chlamydia muridarum, within hollow, vault nanocapsules (MOMP-vaults) that were engineered to bind IgG enhanced immunity. Intranasal immunization...
Previous configurations of biomass conversion technologies based on the use ionic liquids (ILs) suffer from problems such as high operating costs and large amounts water used. There have been recent efforts toward process intensification integration to realize a one-pot approach for biofuel production using certain ILs, but these typically still require pH adjustment and/or dilution after pretreatment before saccharification fermentation. Deep eutectic solvents (DESs) were investigated an...
Understanding tumor-induced angiogenesis is a challenging problem with important consequences for the diagnosis and treatment of cancer. In this study, we define novel function epithelial membrane protein-2 (EMP2) in control angiogenesis. EMP2 functions as an oncogene endometrial cancer, its expression has been linked to decreased survival. Using cancer xenografts, modulation resulted profound changes tumor microvasculature. Under hypoxic conditions, upregulation promoted vascular...
Epithelial membrane protein-2 (EMP2) is upregulated in a number of tumors and therefore remains promising target for mAb-based therapy. In the current study, image-guided therapy an anti-EMP2 mAb was evaluated by PET both syngeneic immunodeficient cancer models expressing different levels EMP2 to enable better understanding its tumor uptake off accumulation clearance. The therapeutic efficacy initially high- low-expressing tumors, reduced load high EMP2-expressing 4T1 HEC-1-A tumors. To...
When operating pins at high data rates, the key problem is to control timing skews (both on- and off-chip) so on can be read in a short time. The of external solved by clocking scheme where clock signals travel same distance between sender receiver that there little skew 600-mV/sub pp/ signals. There are two clocks: one for incoming (RxClk) outgoing (TxClk). A PLL (phase-locked loop) generates properly skewed internal clocks operate bus. consists main loop fine loops (one each receive...
A monolithic 900-MHz CMOS wireless receiver with on-chip RF and IF filters a fully integrated fractional-N synthesizer is presented. Implemented in standard 0.5-/spl mu/m process without any off-chip component, the complete has measured image rejection of 79 dB, sensitivity -90 dBm, an IIP3 -24 noise figure 22 dB power 227 mW chip area 5.7 mm/sup 2/. The achieves phase -118 dBc/Hz at 600 kHz offset settling time less than 150 /spl mu/s.
Chlamydia trachomatis causes STI and reproductive dysfunction worldwide which is not preventable with antibiotics. Identifying a population of endocervical T cells to target in vaccine development would enhance efficacy.Trafficking murine CD4+ lymphocytes muridarum infected genital tract (GT) tissue vivo was measured using adoptive transfer studies fluorescent from integrin β7-/- mice or lack E-selectin on endothelial cells.Murine migration showed that α4β7 significantly reduced trafficking...
Little is known about the role of epithelial membrane protein-2 (EMP2) in breast cancer development or progression. In this study, we tested hypothesis that EMP2 may regulate formation self-renewal stem cells (BCSC) tumor microenvironment.
Dendritic cells (DCs) are central for the induction of T-cell responses needed chlamydial eradication. Here, we report activation two DC subsets: a classical CD11b+ (cDC) and plasmacytoid (pDC) during genital infection with Chlamydia muridarum. Genital induced an influx cDC pDC into tract its draining lymph node (iliac nodes, ILN) as well colocalization T in ILN. C. muridarum also stimulated high levels costimulatory molecules on naïve vivo. In contrast, expressed low most vivo did not...
purpose. Mammalian programmed cell death (PD)-1 is a membrane-associated receptor regulating the balance between T-cell activation, tolerance, and immunopathology; however, its role in neurons has not yet been defined. The hypothesis that PD-1 signaling actively promotes retinal ganglion (RGC) within developing mouse retina was investigated. methods. Mature types expressing were identified by immunofluorescence staining of vertical sections; developmental expression localized immunostaining...
During mouse retina maturation, the final number of retinal ganglion cells (RGCs) is determined by highly regulated programmed cell death. Previous studies demonstrated that immunoregulatory receptor death-1 (PD-1) promotes developmental RGC To identify functional signaling partner(s) for PD-1, we identified expression PD-1 ligands and examined effect ligand on number. We also explored hypothesis development visual circuitry.Characterization brain 1 (PD-L1) were immunofluorescence tissue...
Purpose: Pathologic corneal neovascularization is a major cause of blindness worldwide, and treatment options are currently limited. VEGF one the critical mediators but current anti-VEGF therapies have produced limited results in cornea. Thus, additional therapeutic agents needed to enhance antiangiogenic arsenal. Our group previously demonstrated epithelial membrane protein-2 (EMP2) involvement pathologic angiogenesis multiple cancer models including breast glioblastoma. In this paper, we...
Breast cancer (BC) remains among the most commonly diagnosed cancers in women worldwide. Triple-negative BC (TNBC) is a subset of characterized by aggressive behavior, high risk distant recurrence, and poor overall survival rates. Chemotherapy backbone for treatment patients with TNBC, but outcomes remain compared to other subtypes, part due lack recognized functional targets. In this study, expression tetraspan protein epithelial membrane 2 (EMP2) was explored as predictor TNBC response...
Given their immune-modulating capacity, regulatory T cells (Treg) may be important players in the induction of protective T-cell response (Th1) to genital chlamydial infection. Recent work has demonstrated that plasmacytoid dendritic (pDC) respond infection, and pDC uniquely positioned for Treg during this Here, we present first data demonstrating influx into draining lymph node site infection We found depletion altered numbers nonprotective inflammatory [interferongamma-(IFNgamma)-producing...
A modification of stacked spiral inductors increases the self-resonance frequency by 100% with no additional processing steps, yielding values 5 nH to 266 and frequencies 11.2 GHz 0.5 GHz. Closed-form expressions predicting less than 5% error have also been developed. 1-to-2 transformer consisting 3 spirals achieves a voltage gain 1.8 at 2.5 The structures fabricated in standard CMOS technologies four five metal layers.
A single 5-V supply 256 K EEPROM (electrically erasable programmable read-only memory) was designed, manufactured, and tested. recently developed double-poly n-well CMOS process with 1.25-/spl mu/m minimum feature size successfully used to manufacture this part. Using technology, a 54-/spl mu/m/SUP 2/ cell has been realized. novel autoredundant Q-cell concept in the memory core combined very-high-endurance oxynitride dielectric provides breakthrough needed increase endurance of up one...
Purpose: We investigated the effect of exogenously administered human embryonic stem cell-derived mesenchymal stromal cells (hESC-MSCs) in experimental autoimmune uveitis (EAU) B10.RIII mice, a murine model severe uveitis.Methods: mice were immunized with an uveitogenic peptide, and intraperitoneal injections 5 million hESC-MSCs per animal given on same day. Behavioral light sensitivity assays, histological evaluation, cytokine production, regulatory T analyzed at peak disease.Results:...
Pathologic retinal neovascularization is a potentially blinding consequence seen in many common diseases including diabetic retinopathy, retinopathy of prematurity, and vaso-occlusive diseases. This study investigates epithelial membrane protein 2 (EMP2) its role as possible modulator angiogenesis human pigment epithelium (RPE) under hypoxic conditions. To effects, the RPE cell line ARPE-19 was genetically modified to either overexpress EMP2 or knock down levels, RNA sequencing western blot...
Corneal neovascularization (NV) is a sight-threatening condition often associated with infection, inflammation, prolonged contact lens use, corneal burns, and acute graft rejection. Macrophages recruited to the cornea release nitric oxide (NO) superoxide anion (O2(-)), which react together form highly toxic molecule peroxynitrite (ONOO(-)). The role of ONOO(-) in upregulating multiple angiogenic factors cultured human limbal epithelial (HCLE) cells was investigated.Human were incubated 500...
New methods are needed to eradicate or prevent Chlamydia trachomatis infections. Blockade of epithelial membrane protein 2 (EMP2) by genetic silencing neutralizing polyclonal antibody reduced chlamydial infectivity in vitro. This study tests the prediction that recombinant anti-EMP2 diabody could reduce early infection genital tract vivo. In a murine model, pretreatment with diabody, as compared control significantly bacterial load, tissue production inflammatory cytokines, recruitment...