A5074276799- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Adenosine and Purinergic Signaling
- Plant-Microbe Interactions and Immunity
- Lipid Membrane Structure and Behavior
- Blood Coagulation and Thrombosis Mechanisms
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Hemophilia Treatment and Research
- Lymphoma Diagnosis and Treatment
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Neonatal Respiratory Health Research
- Receptor Mechanisms and Signaling
- Viral Infections and Vectors
- Metabolism and Genetic Disorders
- Plant Parasitism and Resistance
- Ion Transport and Channel Regulation
- Biochemical and Molecular Research
- ATP Synthase and ATPases Research
- Cellular transport and secretion
- Drug Transport and Resistance Mechanisms
- Chronic Myeloid Leukemia Treatments
- Viral Infections and Outbreaks Research
- Neuroscience of respiration and sleep
Semmelweis University
2020-2024
Magyar Agrár- és Élettudományi Egyetem
2022
Zrínyi Miklós National Defence University
2020-2022
Carilion Clinic
2018
Virginia Tech
2018
University of Pecs
2001-2017
University of Szeged
1987-2014
University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca
2007-2012
VA Greater Los Angeles Healthcare System
2009-2012
University of California, Los Angeles
1988-2009
Abstract: A new procedure is described for the isolation of synaptosomes from various parts mammalian brain. This method utilizes an isoosmotic Percoll/sucrose discontinuous gradient and has some advantages over traditionally used synaptosomal techniques: (1) it possible to prepare suitable gradients while retaining isoosmolarity; (2) time preparation remarkably short (approximately 1 h); (3) if necessary, material can be easily removed samples. Intact were recovered 10%/16% (vol/vol)...
Abstract Some filoviruses can be transmitted to humans by zoonotic spillover events from their natural host and filovirus outbreaks have occured with increasing frequency in the last years. The Lloviu virus (LLOV), was identified 2002 Schreiber’s bats ( Miniopterus schreibersii ) Spain subsequently detected Hungary. Here we isolate infectious LLOV blood of a live sampled bat is sequenced cultured sp. kidney cell line SuBK12-08. It furthermore able infect monkey human cells, suggesting that...
Abstract: Intact synaptosomes isolated from mammalian brain tissues (rat, mouse, gerbil, and human) have an ATP hydrolyzing enzyme activity on their external surface. The synaptosomal ecto‐ATPase(s) possesses characteristics consistent with those that been described for ecto‐ATPases of various other cell types. has a high affinity (the apparent K m values are in the range 2–5 × 10 −5 M ), is apparently stimulated equally well by either Mg 2+ or Ca absence any cations. activation constant...
Abstract: An ATP‐hydrolysing activity on the external surface of intact synaptosomes from chicken forebrain has been investigated. The observed ATPase was not due to leakage intracellular activities, artefacts resulting breakage nerve endings during incubation and isolation periods, or possible contamination by other subcellular particles. Disruption resulted in an approximately 2.5‐fold increase basal, Mg 2+ ‐dependent activity, suggesting that plasma membrane acting as a permeability...
Using the radionuclide 65Zn, we have demonstrated direct binding of zinc to purified rhodopsin. 65Zn is eluted with detergent-solubilized rhodopsin from concanavalin A columns and remains bound visual pigment through a subsequent gel-filtration step. Zinc disc membranes highly specific and, ions tested, copper best competitor. Equilibrium-dialysis experiments indicate that forms may increase on bleaching photopigment. These results important implications for studies plays role in retinal...
Cell surface ATPases (ecto‐ATPases or E‐ATPases) hydrolyze extracellular ATP and other nucleotides. Regulation of nucleotide concentration is one their major proposed functions. Based on enzymatic characterization, the E‐ATPases have been divided into two subfamilies, ecto‐ATPases ecto‐ATP‐diphosphohydrolases (ecto‐ATPDases). In presence either Mg 2+ Ca , ecto‐ATPDases, including proteins closely related to CD39, nucleoside diphosphates in addition triphosphates are inhibited by millimolar...
An ecto-ATP diphosphohydrolase (ATPDase) was purified to homogeneity from vesiculosomes shed chicken oviduct. First, the ecto-ATPDase-enriched were concentrated by filtration, differential centrifugation, and exclusion chromatography. Next, nonionic detergent, Nonidet P-40, used extract ecto-ATPDase vesiculosomal membranes, solubilized enzyme further ion exchange (DEAE-Bio-Gel) lentil-lectin-Sepharose 4B In final stage, immunoaffinity chromatography utilized obtain ecto-ATPDase. More than...
The non-protein amino acid β-Aminobutyric (BABA) may trigger the immune responses of plants to various biotic and abiotic stresses leading a long-term resistance (primed state). We present RNA-seq datasets BABA - primed mono- dicotyledonous plants, such as Arabidopsis thaliana Hordeum vulgare. Illumina NextSeq550 sequencing were carried out after 72 h exposure. 87 bp long sequence reads preprocessed treated control samples deposited in NCBI SRA database. Transcriptome de novo assembled each...
Adenylate kinase activity (ATP:AMP-phosphotransferase; EC 2.7.4.3) was studied in various subcellular fractions of rat brain tissues. Because the presence other adenosine nucleotide-utilizing enzymes, adenylate assayed both forward and reverse directions by using coupled enzyme systems a specific inhibitor, P1,P5-di(adenosine-5') pentaphosphate. As expected, highest (2.89 mumol/min/mg protein) detected cytosolic fraction. However, substantial (0.68 mumol/min/mg) also found intact...
Abstract: A study was conducted on the influence of 4‐aminopyridine (4‐AP) radiocalcium uptake and membrane potential rat cortical synaptosomes previously depolarized by biochemical procedures. The initial calcium entry into isolated nerve terminals substantally enhanced in presence 10‐ −4 M 4‐AP potassium‐rich media (60 m ). fast phase potassium‐stimulated involves different kinetic characteristics absence 4‐AP. In 4‐AP, component reached a peak during first 15 s immediately following...
Abstract Purpose: Endometrial cancer is the most common gynecologic malignancy. One promising biomarker epithelial membrane protein 2 (EMP2), and its expression an independent prognostic indicator for tumors with poor clinical outcome expression. The present study assesses suitability of EMP2 as a therapeutic target. Experimental Design: Human monovalent anti-EMP2 antibody fragments were isolated from human phage display library engineered bivalent (diabodies) specificity avidity to both...
The chicken oviduct ecto-ATP diphosphohydrolase (ATPDase), a member of the ecto-ATPase family, was purified to homogeneity previously (Strobel, R. S., Nagy, A. K., Knowles, F., Buegel, J., and Rosenberg, M. O. (1996) <i>J. Biol. Chem.</i> 271, 16323–16331). It is an 80-kDa glycoprotein with high specific activity (approximately 1,000 μmol/min/mg MgATP as substrate) hydrolyzes both nucleoside triphosphates diphosphates. Using amino acid sequence information obtained from enzyme, two partial...
Abstract— Synaptic vesicles were prepared from guinea‐pig cerebral cortex on a continuous D 2 O‐H O(1:1)‐sucrose gradient and purified in the presence of 1 m ‐EGTA by chromatography columns glass beads controlled pore size. As markers, endogenous ACh, NA, dopamine DβH measured. Two distinct populations synaptic recognized between layers 0.2–0.3 ‐ 0.3–0.5 ‐sucrose, which differed each other both electron microscopic appearance transmitter content. The less dense had much higher ACh content...
Severe Acute Respiratory Syndrome Coronavirus 2 is the third highly pathogenic human coronavirus in history. Since emergence Hubei province, China, during late 2019, situation evolved to pandemic level. Following Europe was second epicenter of pandemic. To better comprehend detailed founder mechanisms epidemic evolution Central-Eastern Europe, particularly Hungary, we determined full-length SARS-CoV-2 genomes from 32 clinical samples collected laboratory confirmed COVID-19 patients over...
Triple-negative breast cancer (TNBC) is a subtype of with poor prognosis and limited treatment options. This study evaluates the prognostic value stromal markers in TNBC, focusing on tumor–stroma ratio (TSR) overall stroma (OSR) whole slide images (WSI), as well expression type-I collagen, type-III fibrillin-1 tissue microarrays (TMAs), using both visual assessment digital image analysis (DIA). A total 101 female TNBC patients, primarily treated surgery between 2005 2016, were included. We...
This work aimed to study the plant conditioning effect and mode of action a plant-based biostimulant used in organic farming. new generation biostimulant, named ELICE16INDURES
We previously demonstrated that the major ecto‐nucleoside triphosphate phosphohydrolase in chicken liver membranes is an ecto‐ATP‐diphosphohydrolase (ecto‐ ATPDase) [Caldwell, C., Davis, M.D. & Knowles, A.F. (1999) Arch. Biochem. Biophys . 362 , 46–58]. Enzymatic properties of membrane ecto‐ATPDase are similar to those oviduct we have purified and cloned. Using antibody developed against latter, homogeneity. The enzyme a glycoprotein with molecular mass 85 kDa specific activity ≈ 1000...
Recently, climate change has had an increasing impact on the world. Innate defense mechanisms operating in plants - such as PAMP-triggered Immunity (PTI) help to reduce adverse effects caused by various abiotic and biotic stressors. In this study, of ELICE16INDURES® plant conditioner for organic farming, developed Research Institute Medicinal Plants Herbs Ltd. Budakalász Hungary, were studied a soybean population Northern Hungary. The active compounds ingredients product selected way...