A5061089065- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Epilepsy research and treatment
- Memory and Neural Mechanisms
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Photoreceptor and optogenetics research
- Axon Guidance and Neuronal Signaling
- Neuropeptides and Animal Physiology
- Neuroscience and Neural Engineering
- Neurotransmitter Receptor Influence on Behavior
- GABA and Rice Research
- Vestibular and auditory disorders
- Cerebral Palsy and Movement Disorders
- Neuroendocrine regulation and behavior
- Cholinesterase and Neurodegenerative Diseases
- Infant Development and Preterm Care
- Growth Hormone and Insulin-like Growth Factors
- Tryptophan and brain disorders
- Neuroscience of respiration and sleep
University of California, Los Angeles
2011-2024
Institute of Neurobiology
2007-2020
Scripps Research Institute
2014
VA Greater Los Angeles Healthcare System
2002-2013
United States Department of Veterans Affairs
1990-2013
West Los Angeles College
1991-2011
Wadsworth Center
1994
Peoria campus of the University of Illinois System
1993
Beckman Research Institute
1984-1987
City of Hope
1980-1987
Abstract: Glutamate decarboxylase (GAD) catalyzes the production of γ‐aminobutyric acid (GABA), a major inhibitory neurotransmitter. The mammalian brain contains two forms GAD, with Ms 67,000 and 65,000 (GAD 67 GAD 65 ). Using new antiserum specific for monoclonal antibody , we show that differ in their intraneuronal distributions: is widely distributed throughout neuron, whereas lies primarily axon terminals. In extracts, almost all an active holoenzyme form, saturated its cofactor,...
Abstract A monoclonal antibody to choline acetyltransferase (ChAT), the acetyl‐choline (ACh)‐synthesizing enzyme, has been used localize ChAT within neurons in immunocytochemical preparations of adult rat spinal cord. Morphological details known cholinergic are presented this study, and previously unidentified ChAT‐containing also described. Immunoreactions product was present cell bodies, dendrites, axons, axon terminals, thereby allowing comprehensive descriptions distribution...
Abstract Choline acetyltransferase (ChAT), the acetylcholine‐synthesizing enzyme and a definitive marker for cholinergic neurons, was localized immunocytochemically in motor somatic sensory regions of rat cerebral cortex with monoclonal antibodies. ChAT‐positive (ChAT+) varicose fibers terminal‐like structures were distributed loose network throughout cortex. Some immunoreactive cortical continuous those white matter underlying cortex, many these presumably originated from subcortical...
Abstract Monoclonal antibodies to choline acetyltransferase (ChAT) were used in an immunocytochemical study characterize putative cholinergic neurons and synaptic junctions rat caudate‐putamen. Light microscopy (LM) revealed that ChAT‐positive are distributed throughout the striatum. These cells have large oval or multipolar somata, exhibit three four primary dendrites branch extend long distances. Quantitative analysis of counterstained preparations indicated constitute 1.7% total neuronal...
In cerebellar granule cells, δ subunit-containing GABA A receptors are found exclusively at extrasynaptic sites, but their subcellular distribution in other brain areas is poorly understood. We examined the anatomical localization and physiological activation of these adult mouse dentate gyrus cells. Immunocytochemistry revealed a high density subunits molecular layer much lower cell body layer. At ultrastructural level, immunogold-labeled were edge symmetric synapses on dendrites....
δ Subunit-containing GABA A receptors are located predominantly at nonsynaptic sites in the dentate gyrus where they may play important roles controlling neuronal excitability through tonic inhibition and responses to spillover. Immunohistochemical methods were used determine whether subunit expression was altered after pilocarpine-induced status epilepticus C57BL/6 mice ways that could increase of gyrus. In pilocarpine-treated animals, normal diffuse labeling molecular layer decreased by 4...
The neurotransmitter GABA mediates the majority of rapid inhibition in CNS. Inhibition can occur via conventional mechanism, transient activation subsynaptic GABAA receptors (GABAA-Rs), or continuous high-affinity by low concentrations ambient GABA, leading to "tonic" that control levels excitability and network activity. GABAA-R alpha4 subunit is expressed at high dentate gyrus thalamus suspected contribute extrasynaptic GABAA-R-mediated tonic inhibition. Mice were engineered lack targeted...
Abstract Nonradioactive in situ hybridization methods with digoxigenin‐labeled cRNA probes were used to localize two glutamic acid decarboxylase (GAD) mRNAs rat brain. These encode forms of GAD that both synthesize GABA but differ a number characteristics including their molecular size (65 and 67 kDa). For each mRNA, discrete neuronal labeling high cellular resolution low background staining was obtained most populations known neurons. In addition, the current method revealed differences...
Abstract The δ subunit is a novel of the pentameric γ‐aminobutyric acid (GABA) A receptor that conveys special pharmacological and functional properties to recombinant receptors may be particularly important in mediating tonic inhibition. Mice lack have been produced by gene‐targeting technology, these mice were studied with immunohistochemical immunoblot methods determine whether changes GABA limited deletion or alterations other subunits also present knockout (δ–/–) mice....
Complex changes in GABA A receptors (GABA Rs) animal models of temporal lobe epilepsy during the chronic period include a decrease δ subunit and increases α4 γ2 subunits dentate gyrus. We used postembedding immunogold labeling to determine whether subcellular locations these were also altered pilocarpine-treated epileptic mice, related functional identified electrophysiologically. The ultrastructural studies confirmed at perisynaptic molecular layer gyrus where are critical for tonic...
Alcohol withdrawal syndrome (AWS) symptoms include hyperexcitability, anxiety, and sleep disorders. Chronic intermittent ethanol (CIE) treatment of rats with subsequent (EtOH) reproduced AWS in behavioral assays, which included tolerance to the sleep-inducing effect acute EtOH its maintained anxiolytic effect. Electrophysiological assays demonstrated a CIE-induced long-term loss extrasynaptic GABA A receptor (GABA R) responsiveness gain synaptic R CA1 pyramidal dentate granule neurons that...
Abstract A monoclonal antibody to choline acetyltransferase (ChAT) has been used in an immunocytochemical study of the postnatal development ChAT‐containing neurons cervical and thoracic spinal cord. Specimens from rat pups ranging age 1 28 days (dpn) were studied compared with adult specimens (Barber et al., '84). The established cholinergic neurons, somatic motoneurons sympathetic preganglionic cells, described as that previously unidentified ChAT‐positive dorsal, intermediate, central...
Axonal sprouting of excitatory neurons is frequently observed in temporal lobe epilepsy, but the extent to which inhibitory interneurons undergo similar axonal reorganization remains unclear. The goal this study was determine whether somatostatin (SOM)-expressing stratum (s.) oriens hippocampus exhibit beyond their normal territory and innervate granule cells dentate gyrus a pilocarpine model epilepsy. To obtain selective labeling SOM-expressing s. oriens, Cre recombinase-dependent construct...