A5066764088- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- Epilepsy research and treatment
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Sleep and Wakefulness Research
- Adenosine and Purinergic Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neural Engineering
- Neuroendocrine regulation and behavior
- Birth, Development, and Health
- RNA Research and Splicing
- Tryptophan and brain disorders
- Nerve injury and regeneration
- Anesthesia and Neurotoxicity Research
- Genetics and Neurodevelopmental Disorders
- RNA regulation and disease
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Biochemical Analysis and Sensing Techniques
- Pharmacological Receptor Mechanisms and Effects
Institut de Neurosciences des Systèmes
2012-2023
Inserm
2010-2023
Aix-Marseille Université
2009-2023
University of Zagreb
2022
Institut National de Recherche en Santé Publique
2012
C3J Therapeutics (United States)
2012
Brewster Place
2012
Hôpital de la Timone
2009
Institut de Neurobiologie de la Méditerranée
2000-2008
Institut de Neurophysiopathologie
2007
Two isoforms of glutamic acid decarboxylase (GAD67 and GAD65) their mRNAs were localized in the rat brain by immunohistochemistry nonradioactive situ hybridization methods with digoxigenin-labeled cRNA probes. In most regions, both GAD present neuronal cell bodies as well axon terminals. A few populations neurons, such those reticular nucleus thalamus, exhibited similar body labeling for GADs. However, many that immunoreactive GAD67 often more numerous than GAD65. contrast, density...
In situ hybridization methods were used to determine if glutamic acid decarboxylase (GAD) mRNA-containing neurons within the hilus of dentate gyrus are vulnerable seizure-induced damage in a model chronic seizures. Sprague-Dawley rats injected intraperitoneally with pilocarpine, and hippocampal formation was studied histologically at 1, 2, 4, 8 week intervals after pilocarpine-induced histochemistry, using digoxigenin-labeled GAD cRNA probe, demonstrated substantial decrease number...
Temporal lobe epilepsy (TLE) in humans and animals is associated with axonal sprouting of glutamatergic neurons neosynaptogenesis the hippocampal formation. We examined whether this plasticity excitatory pathways contributes to an increased level excitation CA1 region rats experiencing chronic spontaneous limbic seizures following kainic acid or pilocarpine treatment. In cases, we report extensive pyramidal neurons, many branches entering cell layer stratum radiatum, regions that are not...
Abstract Nonradioactive in situ hybridization methods with digoxigenin‐labeled cRNA probes were used to localize two glutamic acid decarboxylase (GAD) mRNAs rat brain. These encode forms of GAD that both synthesize GABA but differ a number characteristics including their molecular size (65 and 67 kDa). For each mRNA, discrete neuronal labeling high cellular resolution low background staining was obtained most populations known neurons. In addition, the current method revealed differences...
Patients with temporal lobe epilepsy (TLE), the most common form of in adults, often display cognitive deficits. The time course and underlying mechanisms decline remain unknown during epileptogenesis (the process leading to epilepsy). Using rat pilocarpine model TLE, we performed a longitudinal study assess spatial nonspatial performance epileptogenesis. In parallel, monitored interictal-like activity (ILA) hippocampal CA1 region, as well theta oscillations, brain rhythm central numerous...
Abstract In the pilocarpine model of chronic limbic seizures, vulnerability GABAergic interneurons to excitotoxic damage has been reported in hippocampal CA1 region. However, little is known about specific types that degenerate this order characterize these interneurons, we performed quantitative analyses different populations neurons labeled for their peptide or calcium‐binding protein content. Our data demonstrate decrease number GAD mRNA‐containing stratum oriens pilocarpine‐treated rats...
Morphological studies suggest that the primate hippocampus develops extensively before birth, but little is known about its functional development. Patch-clamp recordings of hippocampal neurons and reconstruction biocytin-filled pyramidal cells were performed in slices macaque cynomolgus fetuses delivered by cesarean section. We found during second half gestation, axons dendrites grow intensively hundreds micrometers per day to attain a high level maturity near term. Synaptic currents appear...
Enduring, abnormal expression and function of the ion channel hyperpolarization-activated cyclic adenosine monophosphate gated type 1 (HCN1) occurs in temporal lobe epilepsy (TLE). We examined underlying mechanisms, investigated whether interfering with these mechanisms could modify disease course.Experimental TLE was provoked by kainic acid-induced status epilepticus (SE). HCN1 repression at mRNA, protein, functional levels. Chromatin immunoprecipitation employed to identify transcriptional...
Consumption of certain substances during pregnancy can interfere with brain development, leading to deleterious long-term neurological and cognitive impairments in offspring. To test whether modulators adenosine receptors affect neural we exposed mouse dams a subtype-selective type 2A receptor (A2AR) antagonist or caffeine, naturally occurring antagonist, lactation. We observed delayed migration insertion γ-aminobutyric acid (GABA) neurons into the hippocampal circuitry first postnatal week...
During development, neural circuit formation requires the stabilization of active γ-aminobutyric acid–mediated (GABAergic) synapses and elimination inactive ones. Here, we demonstrate that, although activation postsynaptic GABA type A receptors (GABAARs) adenosine A2A (A2ARs) stabilizes GABAergic synapses, only A2AR is sufficient. Both GABAAR- A2AR-dependent signaling pathways act synergistically to produce 3′,5′-monophosphate through recruitment calcium–calmodulin–adenylyl cyclase pathway....
In the pilocarpine model of chronic limbic seizures, subpopulations glutamic acid decarboxylase (GAD)-containing neurons within hilus dentate gyrus and stratum oriens CA1 hippocampal region are vulnerable to seizure-induced damage. However, many gamma-aminobutyric (GABA) remain in these other regions formation. To determine whether long-term changes occur main metabolic pathway responsible for GABA synthesis remaining neurons, levels mRNA protein labeling two forms GAD (GAD65 GAD67) were...
Patch–clamp recordings of CA1 interneurons and pyramidal cells were performed in hippocampal slices from kainate- or pilocarpine-treated rat models temporal lobe epilepsy. We report that γ-aminobutyric acid (GABA)ergic inhibition neurons is still functional epilepsy because: ( i ) the frequency spontaneous GABAergic currents similar to control ii focal electrical stimulation evokes a hyperpolarization prevents generation action potentials. In paired cells, synchronous interictal activities...
In human most cortical γ-aminobutyric acidergic (GABAergic) neurons are produced in the proliferative zones of dorsal telencephalon contrast to rodents. We report that cynomolgus monkey fetuses GABAergic generated telencephalon, addition region ventral ganglionic eminence (GE), however, with a temporal delay. neuron progenitors labeled for Mash1 and GAD65 were present mainly GE at embryonic days (E) 47–55, entire E64–75. These within locally rather than GE. The lineages display different...
Drebrin A, one of the most abundant neuron-specific F-actin-binding proteins, is found exclusively in dendrites and particularly concentrated dendritic spines receiving excitatory inputs. We investigated role drebrin A synaptic transmission that overexpression augmented glutamatergic transmission, probably through an increase active site density. Interestingly, also affected frequency, amplitude kinetics miniature inhibitory postsynaptic currents (mIPSCs), despite fact GABAergic synapse...
Abstract Specific subunits of γ‐aminobutyric acid (GABA) A receptors may be regulated differentially in animal models temporal lobe epilepsy during the chronic stage. Although several upregulated, other downregulated hippocampal formation. The α5 subunit is particular interest because its relatively selective localization hippocampus and potential role tonic inhibition. In normal rats, immunolabeling was high dendritic layers CA1 CA2 moderate these regions CA3. pilocarpine‐treated rats...
Abstract The mRNAs for two forms of glutamic acid decarboxylase (GAD65 and GAD67) were localized in the rat hippocampal formation by nonradioactive situ hybridization methods with digoxigeninlabeled cRNA probes. Some neurons all layers hippocampus dentate gyrus readily labeled each GAD mRNA, patterns labeling GAD65 GAD67 very similar. All major groups previously described GAD‐and GABA‐containing appeared to be mRNA. Such findings suggest that most GABA contain both mRNAs. When cerebral...