A5066764983- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Neuropeptides and Animal Physiology
- Epilepsy research and treatment
- Microtubule and mitosis dynamics
- Cell Adhesion Molecules Research
- Neonatal and fetal brain pathology
- Cellular Mechanics and Interactions
- Ubiquitin and proteasome pathways
- Proteoglycans and glycosaminoglycans research
- Signaling Pathways in Disease
- 14-3-3 protein interactions
- Drug Transport and Resistance Mechanisms
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Protease and Inhibitor Mechanisms
- Adenosine and Purinergic Signaling
- S100 Proteins and Annexins
- Caveolin-1 and cellular processes
- Barrier Structure and Function Studies
- Pharmacological Receptor Mechanisms and Effects
- Peptidase Inhibition and Analysis
- Fibroblast Growth Factor Research
- Thermal Regulation in Medicine
- Nerve injury and regeneration
Institut de Neurophysiopathologie
2006-2025
Centre National de la Recherche Scientifique
2009-2024
Technological Educational Institute of Eastern Macedonia and Thrace
2024
Aix-Marseille Université
2011-2024
Inserm
1995-2009
Institut de Neurobiologie de la Méditerranée
2001-2009
Hôpital de la Timone
2009
Délégation Paris 5
1996-1999
Université Paris Cité
1996-1999
University of Wisconsin–Madison
1998
Dendritic spines are actin-rich compartments that protrude from the microtubule-rich dendritic shafts of principal neurons. Spines contain receptors and postsynaptic machinery for receiving majority glutamatergic inputs. Recent studies have shown microtubules polymerize into signaling through synaptic NMDA regulates this process. However, mechanisms regulating microtubule dynamics in dendrites remain unclear. Here we show hippocampal neurons male female mice, enter highly localized sites at...
In humans and animal models, temporal lobe epilepsy (TLE) is associated with reorganization of hippocampal neuronal networks, gliosis, neuroinflammation, loss integrity the blood–brain barrier (BBB). More than 30% epilepsies remain intractable, characterization molecular mechanisms involved in BBB dysfunction essential to identification new therapeutic strategies. this work, we induced status epilepticus rats through injection proconvulsant drug pilocarpine, which leads TLE. Using RT-qPCR,...
The quintessential feature of the dendritic microtubule array is its nonuniform pattern polarity orientation. During development dendrite, a population plus end–distal microtubules first appears, and these are subsequently joined by oppositely oriented microtubules. Studies from our laboratory indicate that latter intercalated within their specific transport cell body neuron during critical stage in (Sharp, D.J., W. Yu, P.W. Baas. 1995. J. Cell Biol. 130:93– 104). In addition, we have...
Astrocytes play an active role in the central nervous system and are critically involved astrogliosis, a homotypic response of these cells to disease, injury, associated neuroinflammation. Among numerous molecules processes matrix metalloproteinases (MMPs), family zinc-dependent endopeptidases, secreted or membrane-bound, that regulate by proteolytic cleavage extracellular matrix, cytokines, chemokines, cell adhesion molecules, plasma membrane receptors. MMP activity is tightly regulated...
Drebrin A, one of the most abundant neuron-specific F-actin-binding proteins, is found exclusively in dendrites and particularly concentrated dendritic spines receiving excitatory inputs. We investigated role drebrin A synaptic transmission that overexpression augmented glutamatergic transmission, probably through an increase active site density. Interestingly, also affected frequency, amplitude kinetics miniature inhibitory postsynaptic currents (mIPSCs), despite fact GABAergic synapse...
Neurotensin (NT) is a 13-amino acid neuropeptide widely distributed in the CNS that has been involved pathophysiology of many neural and psychiatric disorders. There are three known neurotensin receptors (NTSRs), which mediate multiple actions, form neurotensinergic system conjunction with NT. NTSR1 main mediator NT, displaying effects both periphery, while NTSR2 mainly expressed brain NTSR3 broader expression pattern. In this review, we bring together up-to-date studies showing an...
Abstract Kainate (KA) is a potent neuroexcitatory agent in several areas of the adult brain, with convulsant and excitotoxic properties that increase as ontogeny proceeds. Besides its depolarizing actions, KA may enhance intracellular accumulation Ca 2+ to promote selective neuronal damage. The effects are mediated by specific receptors recently considered be involved fast neurotransmission can activated synaptically. receptors, e.g. GluR5 GluR6 have been characterized molecular cloning....
Preclinical and clinical studies show that mild to moderate hypothermia is neuroprotective in sudden cardiac arrest, ischemic stroke, perinatal hypoxia/ischemia, traumatic brain injury, seizures. Induction of largely involves physical cooling therapies, which induce several complications, while some molecules have shown be efficient pharmacologically induced (PIH). Neurotensin (NT), a 13 amino acid neuropeptide regulates body temperature, interacts with various receptors mediate its...
It is well established that the microtubules of mitotic spindle are organized by a variety motor proteins, and it appears same motors or closely related variants organize in postmitotic neuron. Specifically, cytoplasmic dynein kinesin-related known as CHO1/MKLP1 used within spindle, recent studies suggest they also essential for establishment axonal dendritic microtubule arrays Other required to tightly regulate behaviors attractive speculate these might help Here we show homolog Eg5...
Background Tissue inhibitor of metalloproteinases-1 (TIMP-1) displays pleiotropic activities, both dependent and independent its inhibitory activity on matrix metalloproteinases (MMPs). In the central nervous system (CNS), TIMP-1 is strongly upregulated in reactive astrocytes cortical neurons following excitotoxic/inflammatory stimuli, but no information exists effects growth morphology neurons. Principal Findings We found that 24 h incubation with recombinant induced a 35% reduction neurite...
Abstract The reorganizations of the overall intrinsic glutamatergic and γ‐aminobutyric acid (GABA)‐ergic hippocampal networks as well time course these during development pilocarpine‐induced temporal lobe epilepsy were studied with in situ hybridization immunohistochemistry experiments for vesicular glutamate transporter 1 (VGLUT1) GABA (VGAT). These transporters are particularly interesting specific markers GABAergic neurons, respectively, whose expression levels could reflect demand...
Abstract Olfactory ensheathing cells (OECs) are unique glia found only in the olfactory system. They retain exceptional plasticity and support neurogenesis retargeting across PNS:CNS boundary OECs have been shown to improve functional outcome when transplanted into rodents with spinal cord injury. The growth‐promoting properties of implanted encompass their ability migrate through scar tissue render it more permissive for axonal outgrowth, but underlying molecular mechanisms remain poorly...
In mesial temporal lobe epilepsy (MTLE), spontaneous seizures likely originate from a multi-structural epileptogenic zone, including several regions of the limbic system connected to hippocampal formation. this study, we investigate structural connectivity between supramammillary nucleus (SuM) and dentate gyrus (DG) in model MTLE induced by pilocarpine rat. This hypothalamic nucleus, which provides major extracortical projections formation, plays key role regulation hippocampus-dependent...
Drebrin A is one of the most abundant neuron-specific binding proteins F-actin and its expression increased in parallel with synapse formation. particularly concentrated dendritic spines, postsynaptic sides excitatory glutamatergic synapses. More recently, Ferhat colleagues reported functional role drebrin regulating synaptic transmission. Indeed, our study showed that overexpression induced an increase but not GABAergic synapses resulted alteration normal excitatory-inhibitory ratio favor...
Abstract Neurotensin (NT) acts as a primary neurotransmitter and neuromodulator in the CNS has been involved number of pathologies including epilepsy. NT mediates its central peripheral effects by interacting with NTSR1, NTSR2, Sort1/NTSR3 receptor subtypes. To date, little is known about precise expression receptors brain neural cells their regulation pathology. In present work, we studied cellular distribution NTSR2 protein rat hippocampus questioned whether was modulated conditions...
Temporal lobe epilepsy (TLE) is associated with reorganization of hippocampal neuronal net-works, gliosis, neuroinflammation, loss integrity the blood-brain barrier (BBB) in humans and animal models. More than 30% epilepsies remain intractable characterization molecular mechanisms involved BBB dysfunction essential to identification new thera-peutic strategies. In this work, we induced status epilepticus rats by injection proconvul-sant drug pilocarpine that leads TLE. Using RT-qPCR, double...
Abstract: Basic fibroblast growth factor (bFGF) is present in the developing rat brain and has been shown to provide critical trophic support for hippocampal neurons culture. The influence of bFGF on expression mRNAs encoding transcription factors zif/268 c‐ fos was studied primary cultures (derived from embryos) using reverse transcription‐coupled PCR. In these grown 3 days absence serum, causes a dramatic transient increase levels , within 15 30 min, respectively. A similar induction two...
Abstract We have previously shown that, in HEK 293 cells, overexpression of acidic calponin, an actin‐binding protein, induces remodeling actin filaments, leading to a change cell morphology. In addition, this protein is found dendritic spines adult hippocampal neurons. hypothesized that plays role regulating actin‐based filaments during spine plasticity. To assess hypothesis, the pilocarpine model temporal lobe epilepsy was selected because important reorganization glutamatergic network,...
Abstract The kinesin‐related motor protein CHO1/MKLP1 was initially thought to be expressed only in mitotic cells, where it presumably transports oppositely oriented microtubules relative one another the spindle mid‐zone. We have recently shown that is also cultured neuronal enriched developing dendrites ( Sharp et al . 1997a ) J. Cell Biol ., 138, 833–843]. putative function of these postmitotic cells intercalate minus‐end‐distal among within dendrites, thereby establishing their...