A5008050888- Neuroscience and Neuropharmacology Research
- Zebrafish Biomedical Research Applications
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Neuroscience of respiration and sleep
- Vestibular and auditory disorders
- Nerve injury and regeneration
- Spinal Cord Injury Research
- Ion channel regulation and function
- Pain Mechanisms and Treatments
- Hearing, Cochlea, Tinnitus, Genetics
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Sleep and Wakefulness Research
- Neurogenesis and neuroplasticity mechanisms
- Memory and Neural Mechanisms
- Neurotransmitter Receptor Influence on Behavior
- Calpain Protease Function and Regulation
- Muscle activation and electromyography studies
- Lipid Membrane Structure and Behavior
- Neonatal and fetal brain pathology
- Neuroendocrine regulation and behavior
- Amyotrophic Lateral Sclerosis Research
- stochastic dynamics and bifurcation
- Biochemical effects in animals
Institut de Neurosciences de la Timone
2013-2024
Aix-Marseille Université
2011-2024
Centre National de la Recherche Scientifique
2010-2024
Hôpital Sainte-Marguerite
1987-2004
University of Gothenburg
1988-1992
Inserm
1986-1987
In healthy adults, activation of γ-aminobutyric acid (GABA) A and glycine receptors inhibits neurons as a result low intracellular chloride concentration ([Cl – ] i ), which is maintained by the potassium-chloride cotransporter KCC2. reduction KCC2 expression or function implicated in pathogenesis several neurological disorders, including spasticity chronic pain following spinal cord injury (SCI). Given critical role regulating strength robustness inhibition, identifying tools that may...
Abstract A number of noradrenaline and serotonin agonists were tested to investigate which them replicate the depressive actions monoamines on transmission from group II muscle afferents in cat spinal cord. The applied ionophoretically at two sites maximal monosynaptic focal field potentials are evoked afferents—in intermediate zone dorsal horn 4th 5th lumbar segments. Their effects estimated changes amplitude potentials. compounds fell into three categories according site they depressed...
Abstract The morphology of midlumbar interneurones with peripheral input from group II muscle afferents was analysed after intracellular injection horseradish peroxidase (HRP). Twenty‐three were stained intrasomatically and five others intra‐axonally. majority (10 13) located in lamina VII (intermediate zone ventral horn interneurones) found to project ipsilaterally. They had medium‐sized somata dendrites projecting radially over a distance more than 1 mm. All these neurones axons that...
Abstract Several studies suggest that neurons from the lateral region of SuM (SuML) innervating dorsal dentate gyrus (DG) display a dual GABAergic and glutamatergic transmission are specifically activated during paradoxical (REM) sleep (PS). The objective present study is to characterize anatomical, neurochemical electrophysiological properties SuML-DG projection determine how they control DG oscillations neuronal activation PS other vigilance states. For this purpose, we combine structural...
Abstract We characterized the interneurons involved in control of ankle extensor (triceps surae [TS] muscles) motoneurons (MNs) lumbar enlargement mouse neonates by retrograde transneuronal tracing using rabies virus (RV). Examination kinetics transfer at sequential intervals post inoculation enabled us to determine time window during which only first‐order interneurons, i.e., likely monosynaptically connected MNs (last‐order [loINs]) were RV‐infected. The infection network resulted...
Vestibulospinal pathways activate contralateral motoneurons (MNs) in the thoracolumbar spinal cord of neonatal mouse exclusively via axons descending ipsilaterally from vestibular nuclei lateral vestibulospinal tract (LVST; Kasumacic et al., 2010). Here we investigate how transmission LVST to MNs is mediated by commissural interneurons (dCINs) different segments. We test polysynaptic nature this crossed projection assessing LVST-mediated ventral root (VR) response latencies, manipulating...
To assess the organization and functional development of vestibulospinal inputs to cervical motoneurons (MNs), we have used electrophysiology (ventral root electromyographic [EMG] recording), calcium imaging, trans-synaptic rabies virus (RV) conventional retrograde tracing immunohistochemistry in neonatal mouse. By stimulating VIIIth nerve electrically while recording synaptically mediated responses MNs, characterized from three tracts, separate ipsilateral contralateral medial tracts...
Summary Amyotrophic lateral sclerosis (ALS) is a devastating motoneuronal degenerative disease, which inevitably fatal in adults. ALS characterized by an extensive loss of motoneurons the cerebrospinal axis, except for those that control eye movements and bladder contraction. The reason this selectivity not known. Systematic differences have been found organization excitatory synaptic transmission ALS-resistant vs. ALS-susceptible motor nuclei. However, although express high levels glycine...
Abstract Neurones of origin the ventral spinocerebellar tract were stained with intracellularly applied horseradish peroxidase to investigate whether they give off any initial axon collaterals. The neurones located in fourth and fifth lumbar segments identified by their antidromic activation following stimulation contralateral superior cerebellar peduncle. Nine 23 well‐stained axons found collaterals soon after crossed midline. entered horn branched within lamina VII dorsal part VIII....
Maturation of inhibitory postsynaptic transmission onto motoneurons in the rat occurs during perinatal period, a time window which pathways arising from brainstem reach lumbar enlargement spinal cord. There is developmental switch miniature IPSCs (mIPSCs) predominantly long-duration GABAergic to short-duration glycinergic events. We investigated effects complete neonatal [postnatal day 0 (P0)] cord transection (SCT) on expression Glycine and GABA A receptor subunits (GlyR R subunits)...
In mesial temporal lobe epilepsy (MTLE), spontaneous seizures likely originate from a multi-structural epileptogenic zone, including several regions of the limbic system connected to hippocampal formation. this study, we investigate structural connectivity between supramammillary nucleus (SuM) and dentate gyrus (DG) in model MTLE induced by pilocarpine rat. This hypothalamic nucleus, which provides major extracortical projections formation, plays key role regulation hippocampus-dependent...
Abstract Following reconstruction with high spatial resolution of the 3‐D geometry dendritic arborizations two abducens motoneurons, we simulated distribution electrotonic voltage over whole tree. Here, demonstrate that complex stochastic structure both motoneurons can be reduced to a statistically significant small set well discriminated clusters. These clusters are formed by branches belonging different dendrites neuron but similar properties. A cluster analysis was performed estimate...
Abstract We describe how the stochastic geometry of dendritic arborization a single identified motoneuron rat affects local details its electrotonic structure. After describing 3D at high spatial resolution, we simulate distribution voltage gradients along branches under steady‐state and transient conditions. show that variations in diameters asymmetric branchings determine non‐monotonous features heterogeneous This is defined by decay expressed as function somatofugal paths physical...
Motor control and body representation in the central nervous system (CNS) as well musculoskeletal architecture physiology are shaped during development by sensorimotor experience feedback, but emergence of locomotor disorders maturation their persistence over time remain a matter debate absence brain damage. By using transient immobilization hind limbs, we investigated enduring impact postnatal restriction (SMR) on gait posture treadmill, age-related changes locomotion, histopathology...
Abstract In newborn mice of the control [C3H/HeJ (C3H)] and monoamine oxidase A‐deficient (Tg8) strains, in which levels endogenous serotonin (5‐HT) were drastically increased, we investigated how 5‐HT system dysregulation affected maturation phrenic motoneurons (PhMns), innervate diaphragm. First, using immunocytochemistry confocal microscopy, observed a 2A receptor (5‐HT ‐R) expression PhMns both C3H Tg8 neonates at somatic dendritic levels, whereas 1B was only level. We interactions...
Premotor inhibitory neurons responsible for the decrease in firing discharge during fast or slow eye movements selectively target cell bodies and dendrites of abducens motoneurons. Gamma-aminobutyric acid (GABA) glycine, main synaptic neurotransmitters central nervous system, act via glycine GABAA receptors, assembled from various types subunits, which determine kinetics currents mediated. Therefore, our hypothesis was that expression receptors on somatic dendritic compartments, involved...
Lead poisoning is one of the most significant health problem environmental origin. It known to cause different damages in central and peripheral nervous system which could be represented by several neurophysiological behavioral symptoms. In this study we firstly investigated effect lead prenatal exposure rats (3g/L), from neonatal young age, on motor/sensory performances, excitability spinal cord gaits during development. Then evaluated neuroprotective effects curcumin I (Cur I) against...