A5061931865- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Neuroscience of respiration and sleep
- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Neurobiology and Insect Physiology Research
- Receptor Mechanisms and Signaling
- Hearing, Cochlea, Tinnitus, Genetics
- Computational Drug Discovery Methods
- Zebrafish Biomedical Research Applications
- Hormonal and reproductive studies
- Photoreceptor and optogenetics research
- Nerve injury and regeneration
- Essential Oils and Antimicrobial Activity
- Anesthesia and Neurotoxicity Research
- Infant Health and Development
- Phytochemistry and Biological Activities
- Diet and metabolism studies
- Plant and Biological Electrophysiology Studies
- French Language Learning Methods
- Cellular transport and secretion
Institut des Neurosciences Cellulaires et Intégratives
2013-2024
Université de Strasbourg
2009-2023
Centre National de la Recherche Scientifique
1998-2022
Friedrich Miescher Institute
2010
Keele University
2000-2010
Institut de Neurobiologie de la Méditerranée
2006
University of Bern
2002-2005
Université de franche-comté
1996-1998
Université de Lorraine
1998
Laboratoire de Neurosciences Cognitives
1998
Abstract The hypothalamic neuropeptide oxytocin (OT) exerts prominent analgesic effects via central and peripheral action. However, the precise pathways recruited by OT are largely elusive. Here we discovered a subset of neurons whose projections preferentially terminate on receptor (OTR)-expressing in ventrolateral periaqueductal gray (vlPAG). Using newly generated line transgenic rats (OTR-IRES-Cre), determined that most vlPAG OTR expressing cells targeted GABAergic. Ex vivo stimulation...
The persistent sodium current ( I NaP ) is known to play a role in rhythm generation different systems. Here, we investigated its contribution locomotor pattern the neonatal rat spinal cord. network mainly located ventromedial gray matter of upper lumbar segments. By means whole cell recordings slices, characterized membrane and biophysical properties interneurons this area. Compared with motoneurons, were more excitable, because higher input resistance time constant, displayed lower firing...
Cultures of neurons from rat neocortex exhibit spontaneous, temporally patterned, network activity. Such a distributed activity in vitro constitutes possible framework for combining theoretical and experimental approaches, linking the single-neuron discharge properties to phenomena. In this work, we addressed issue closing loop, identification single-cell prediction collective Thus, compared these predictions with spontaneously emerging vitro, detected by substrate arrays microelectrodes....
Oxytocin possesses several physiological and social functions, among which an important analgesic effect. For this purpose, oxytocin binds mainly to its unique receptor, both in the central nervous system peripheral nociceptive terminal axon skin. However, despite interesting properties current use clinics facilitate labor, is not used pain treatment. Indeed, it rapidly metabolized, with a half-life blood circulation estimated at five minutes cerebrospinal fluid around twenty humans rats....
Abstract Disinhibition reliably induces regular synchronous bursting in networks of spinal interneurons culture as well the intact cord. We have combined extracellular multisite recording using multielectrode arrays with whole cell recordings to investigate mechanisms involved organotypic and dissociated cultures from cords embryonic rats. Network bursts induced depolarization spikes single neurons, which were mediated by recurrent excitation through glutamatergic synaptic transmission. When...
Abstract We have shown previously that rhythm generation in disinhibited spinal networks is based on intrinsic spiking, network recruitment and a refractory period following the bursts. This mainly electrogenic Na/K pump activity. In present work, we investigated role of persistent sodium current (I NaP ) bursting using patch‐clamp multielectrode array recordings. detected I exclusively spiking cells. The blockade by riluzole suppressed silencing cells suppressing recruitment. produced...
Fast Inhibitory controls mediated by glycine (GlyRs) and GABAA receptors (GABAARs) play an important role to prevent the apparition of pathological pain symptoms allodynia hyperalgesia. The use positive allosteric modulators these receptors, specifically expressed in spinal cord, may represent interesting strategy limit or block expression. In this study, we have used stereoisomers progesterone metabolites, acting only via non-genomic effects, order evaluate contribution GlyRs GABAARs for...
Background Growing evidence in the literature shows that oxytocin (OT) has a strong spinal anti-nociceptive action. Oxytocinergic axons originating from subpopulation of paraventricular hypothalamic neurons establish synaptic contacts with lamina II interneurons but little is known about functional role OT respect to neuronal firing and excitability. Results Using patch-clamp technique, we have recorded acute transverse lumbar cord slices rats (15 30 days old) analyzed effects on action...
Disinhibition-induced bursting activity in cultures of fetal rat spinal cord is mainly controlled by intrinsic spiking with subsequent recurrent excitation the network through glutamate synaptic transmission, and autoregulation neuronal excitability. Here we investigated contribution electrogenic Na/K pump to excitability using extracellular recordings multielectrode arrays (MEAs) intracellular whole cell from interneurons. The blockade strophanthidin led an immediate transient increase...
Context: Menthol, the main monoterpene found in Mentha piperita L. (M. piperita) is known to modulate nociceptive threshold and present different curative preparations that reduce sensory hypersensitivities pain conditions. While for pulegone, a menthol-like monoterpene, only limited number of studies focus on its putative analgesic effects, pulegone most abundant Calamintha nepeta (L.) Savi (C. nepeta), plant Lamiaceae family used traditional medicine alleviate rheumatic disorders, which...
Inflammatory and degenerative diseases of the joint are major causes chronic pain. Long-lasting pain symptoms thought to result from a central sensitization nociceptive circuits. These processes include activation microglia spinal disinhibition. Using monoarthritic rat model pain, we tried potentiate neural inhibition by using etifoxine (EFX), nonbenzodiazepine anxiolytic that acts as an allosteric-positive modulator gamma-aminobutyric acid type A (GABAA) receptor function. Interestingly,...
Abstract The nociceptive system of rodents is not fully developed and functional at birth. Specifically, C fibers transmitting peripheral information establish synaptic connections in the spinal cord already during embryonic period that only become after Here, we studied consequences neonatal maternal deprivation ( NMD , 3 h/day, P2–P12) on establishment fiber‐mediated neurotransmission pain‐related behavior. In vivo recording revealed excitation neurons could be observed P14 control but...
Pain is an emotion and neuropathic pain symptoms are modulated by supraspinal structures such as the amygdala. The central nucleus of amygdala often called 'nociceptive amygdala', but little known about role basolateral Here, we monitored mechanical nociceptive thresholds in a mouse model infused modulators glutamate/GABAergic transmission (BLA) via chronically-implanted cannulas. We found that N-methyl-D-aspartate-type glutamate receptor antagonist (MK-801) exerted potent antiallodynic...
Abstract Corticosterone ( CORT ) is a glucocorticoid produced by adrenal glands under the control of hypothalamic–pituitary–adrenal axis. Circulating can enter central nervous system and be reduced to neuroactive 3α5α‐reduced steroids, which modulate GABA A receptors. In dorsal spinal cord, ergic transmission modulates integration nociceptive information. It has been shown that enhancing inhibitory alleviates hyperalgesia allodynia. Therefore, neuronal network pivotal target counteract pain...
Abstract N ‐methyl‐ d ‐aspartic acid (NMDA) receptors are implicated in fictive locomotion; however, their precise role there is not clear. In cultures of dissociated cells from foetal rat spinal cord, synchronous bursting (but locomotion) can be induced by disinhibition, which produced blocking glycinergic and γ‐aminobutyric (GABA) A ‐dependent synaptic conductances. this study, we investigate the NMDA‐R rhythm generation during disinhibition with multielectrode arrays patch‐clamp. We...
Background Lithium is widely used to treat bipolar disorders and displays mood stabilizing properties. In addition, lithium relieves painful cluster headaches has a strong analgesic effect in neuropathic pain rat models. Objectives To investigate the of on cuff model pain. Methods We behavioral pharmacological approaches study single injection wild-type mu opioid receptor (MOR) null cuffed mice. Mass spectrometry enzyme-linked immunosorbent assay allowed measure levels endogenous MOR agonist...
Background and Purpose Chronic administration of medication can significantly affect metabolic enzymes leading to physiological adaptations. Morphine metabolism in the liver has been extensively studied following acute morphine treatment, but such processes CNS are poorly characterized. Long‐term treatment is limited by development tolerance, resulting a decrease its analgesic effect. Whether or not tolerance affects vivo brain blood–brain barrier (BBB) permeability remains major question....
Disinhibition of rat spinal networks induces a spontaneous rhythmic bursting activity. The major mechanisms involved in the generation such are intrinsic neuronal firing subpopulation interneurons, recruitment network by recurrent excitation, and autoregulation excitability. We have combined whole cell recording with calcium imaging flash photolysis caged-calcium to investigate contribution [Ca 2+ ] i rhythmogenesis. found that mainly enters neurons through voltage-activated channels...
Abstract The hypothalamic neuropeptide, oxytocin (OT), exerts prominent analgesic effects via central and peripheral action. Here we discovered a novel subset of OT neurons whose projections preferentially terminate on receptor (OTR)-expressing in the ventrolateral periaqueductal gray (vlPAG). Using newly generated line transgenic rats (OTR-IRES-Cre), determined that most vlPAG OTR expressing cells being targeted by are GABAergic nature. Both optogenetically-evoked axonal release as well...