A5002945906- Trauma and Emergency Care Studies
- Gun Ownership and Violence Research
- Microtubule and mitosis dynamics
- Injury Epidemiology and Prevention
- Cellular Mechanics and Interactions
- Suicide and Self-Harm Studies
- Neurogenesis and neuroplasticity mechanisms
- Cardiac Arrest and Resuscitation
- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Emergency and Acute Care Studies
- Axon Guidance and Neuronal Signaling
- Burn Injury Management and Outcomes
- Restraint-Related Deaths
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Traumatic Brain Injury and Neurovascular Disturbances
- Trauma Management and Diagnosis
- Photoreceptor and optogenetics research
- Autopsy Techniques and Outcomes
- Surgical Simulation and Training
- Neuroscience and Neural Engineering
- Cell Adhesion Molecules Research
- Thermal Regulation in Medicine
- RNA Interference and Gene Delivery
- Minimally Invasive Surgical Techniques
The University of Texas Health Science Center at San Antonio
2022-2024
The University of Texas Health Science Center at Houston
2022-2024
Hennepin Healthcare Research Institute
2021-2024
University of Wisconsin–Madison
2011-2016
Medical College of Wisconsin
2015
Dendritic spines are actin-rich compartments that protrude from the microtubule-rich dendritic shafts of principal neurons. Spines contain receptors and postsynaptic machinery for receiving majority glutamatergic inputs. Recent studies have shown microtubules polymerize into signaling through synaptic NMDA regulates this process. However, mechanisms regulating microtubule dynamics in dendrites remain unclear. Here we show hippocampal neurons male female mice, enter highly localized sites at...
Microtubules (MTs) are capable of entering dendritic spines in mature hippocampal neurons through dynamic polymerization. Although these MT invasions directly associated with neuronal activity, their function remains unknown. Here we demonstrate mouse that entries into regulate the increase postsynaptic density-95 (PSD-95) protein after brain-derived neurotrophic factor (BDNF) treatment. Using multiwavelength total internal reflectance fluorescence microscopy, show BDNF prolonged average...
Aortic occlusion (AO) is a lifesaving therapy for the treatment of severe traumatic hemorrhagic shock; however, there remains controversy whether AO should be accomplished via resuscitative thoracotomy (RT) or endovascular balloon aorta (REBOA) in zone 1.To compare outcomes RT vs REBOA 1.This was comparative effectiveness research study using multicenter registry postinjury from October 2013 to September 2021. 1 (above celiac artery) compared with performed emergency department facilities...
Most excitatory synaptic terminals in the brain impinge on dendritic spines. We and others have recently shown that dynamic microtubules (MTs) enter spines from shaft. However, a direct role for MTs long-lasting spine plasticity has yet to be demonstrated it remains unclear whether MT-spine invasions are directly influenced by activity. Lasting changes morphology strength can triggered activation of NMDA receptors (NMDARs) associated with learning memory processes. To determine involved...
Hippocampal and cortical neurons have been used extensively to study central nervous system (CNS) neuronal polarization, axon/dendrite outgrowth, synapse formation function. An advantage of culturing these is that they readily polarize, forming distinctive axons dendrites, on a two dimensional substrate at very low densities. This property has made them extremely useful for determining many aspects development. Furthermore, by providing glial conditioning will continue develop, functional...
Growth cones interact with the extracellular matrix (ECM) through integrin receptors at adhesion sites termed point contacts. Point contact adhesions link ECM proteins to actin cytoskeleton numerous adaptor and signaling proteins. One presumed function of growth cone contacts is restrain or “clutch” myosin-II-based filamentous (F-actin) retrograde flow (RF) promote leading edge membrane protrusion. In motile non-neuronal cells, myosin-II binds exerts force upon filaments edge, where...
Abstract Introduction Approximately 30% of severe frostbite injuries result in amputation. Thrombolytic therapy (tPA) is used to reduce tissue loss following injury. This study evaluates factors impacting the tPA effectiveness using post-tPA perfusion imaging and amputation level as outcome measures. We hypothesize that stratifying tPA-treated patients into full, partial, non-responders will allow for a more nuanced assessment treatment efficacy. Methods reviewed prospectively maintained...
Abstract Introduction Severe frostbite injury can lead to significant long-term functional impairments. This study aims assess function and symptom resolution in upper vs. lower extremity frostbite. We hypothesize that is slower need for assistance higher injury. Methods A longitudinal cohort of 182 severe injured patients, defined with a post-rewarming perfusion deficit on imaging and/or clinical diagnosis, included 137 patients isolated or Analyses were stratified by amputation only those...
Abstract Introduction Extreme weather events are increasing in regions unaccustomed to freezing temperatures, placing more children at risk for frostbite and cold-related injuries, which can lead significant morbidity mortality. Many medical centers lack established management protocols, where early identification treatment essential. This study reviews pediatric cold-weather injuries across multiple databases delineate the extent of these identify geographic areas targeted intervention...
Abstract Introduction Thrombolytics (tPA) improve limb and digit salvage following severe frostbite injury, however disability tPA therapy is largely undescribed. Our aim was to evaluate functional status, pain, in a large cohort of patients treated with without tPA, hypothesizing no significant differences between the two groups. Methods Longitudinal study injured over winters 2013-20. Therapy notes were reviewed at discharge follow-up. Outcomes categorized including issues balance, grip or...
Abstract Introduction Severe frostbite injury can lead to long-term physical disability, particularly in patients who require amputation. While interventions often focus on limb salvage, understanding functional outcomes is crucial for planning rehabilitation and improving post-amputation care. This study aimed evaluate the status, pain levels, disability with severe frostbite. Methods retrospective included 59 injured amputations. Charts were reviewed at discharge, subsequent...
BACKGROUND Health insurance and race impact mortality discharge outcomes in the general trauma population. It remains unclear if disparities exist by and/or following firearm injuries. The purpose of this study was to assess differences based on status METHODS National Trauma Data Bank (2007–2016) queried for injuries International Classification Diseases, Ninth/Tenth Revision , Ecodes. Patients with known disposition, age (18–64 years), race, were included analysis (N = 120,005). To...
CIP4, a member of the F-BAR family proteins, plays important roles in variety cellular events by regulating both membrane and actin dynamics. In many cell types CIP4 functions vesicle formation, endocytosis tubulation. However, recent data indicate that is also involved protrusion some types, including cancer cells (lamellipodia invadopodia) neurons (ribbed lamellipodia veils). neurons, localizes specifically to extending protrusions limit neurite outgrowth early development. The mechanism...
The role of calcium is ubiquitous in human physiology. Emerging evidence suggests that the lethal triad be revised to include hypocalcemia (hypoCa) and thus known as diamond . There are data showing traumatic injury may result hypoCa independent from mechanism chelation by citrate-based blood preservatives. Minimal literature exists analyzing pediatric trauma patients. We hypothesize there an association with increased product requirements mortality.A retrospective cohort study severely...
Adhesive micro-lines of various sub-cellular geometries were created using a non-traditional micro stamping technique. This technique employed the use commercially available diffraction gratings as molds for stamps, method which is quick and inexpensive, could easily be adopted patterning tool in variety research efforts. The atypical saw-tooth profile stamps enabled unique degree control flexibility over patterned line gap widths. Cortical neurons cultured on poly-lysine PDMS exhibit...
Hippocampal and cortical neurons have been used extensively to study central nervous system (CNS) neuronal polarization, axon/dendrite outgrowth, synapse formation function. An advantage of culturing these is that they readily polarize, forming distinctive axons dendrites, on a two dimensional substrate at very low densities. This property has made them extremely useful for determining many aspects development. Furthermore, by providing glial conditioning will continue develop, functional...
Patients discharged against medical advice do not receive adequate treatment and have a greater risk of readmission. This study assessed the rate discharges following assault-related penetrating trauma, with secondary aims to evaluate long term pre/post-injury hospitalizations mortality.
Abstract Severe hypothermia and frostbite can result in significant morbidity mortality. We present a case of patient with severe due to cold exposure after snowmobile crash. He presented cardiac arrest core temperature 19°C requiring prolonged cardiopulmonary resuscitation, active internal rewarming, venoarterial extracorporeal membrane oxygenation, subsequently amputations all four extremities. Although be fatal condition, the quick action Emergency Medical Services, emergency physicians,...