A5069289540- Cancer Immunotherapy and Biomarkers
- Retinal Diseases and Treatments
- Melanoma and MAPK Pathways
- T-cell and B-cell Immunology
- Retinopathy of Prematurity Studies
- Retinal Development and Disorders
- Immune Cell Function and Interaction
- Ocular Diseases and Behçet’s Syndrome
- Advanced Fluorescence Microscopy Techniques
- Ocular Oncology and Treatments
- Cancer Genomics and Diagnostics
- Neonatal Respiratory Health Research
- Ocular Infections and Treatments
- Retinal and Optic Conditions
- Genomic variations and chromosomal abnormalities
- Glaucoma and retinal disorders
- Prenatal Screening and Diagnostics
- Cytomegalovirus and herpesvirus research
- Brain Metastases and Treatment
- Mitochondrial Function and Pathology
- Retinal and Macular Surgery
- Advanced biosensing and bioanalysis techniques
- Cutaneous Melanoma Detection and Management
- Anesthesia and Neurotoxicity Research
- Animal Virus Infections Studies
University of California, Los Angeles
2019-2024
Doheny Eye Institute
2019-2020
Washington University in St. Louis
2013-2019
UCLA Health
2019
Ariadne Diagnostics (United States)
2012
Complete Genomics (United States)
2009
Toward $1000 Genomes The ability to generate human genome sequence data that is complete, accurate, and inexpensive a necessary prerequisite perform genome-wide disease association studies. Drmanac et al. (p. 78 , published online 5 November) present technique advancing toward this goal. method uses Type IIS endonucleases incorporate short oligonucleotides within set of randomly sheared circularized DNA. DNA polymerase then generates concatenated copies the circular leading formation compact...
ABSTRACT Objective To develop a novel prenatal assay based on selective analysis of cell‐free DNA in maternal blood for evaluation fetal Trisomy 21 (T21) and 18 (T18). Methods Two hundred ninety‐eight pregnancies, including 39 T21 seven T18 confirmed aneuploidies, were analyzed using novel, highly multiplexed assay, termed digital selected regions (DANSR™). Cell‐free from samples was DANSR assays loci chromosomes 18. Products 96 separate patients pooled sequenced together. A standard Z ‐test...
NK cell activation is controlled by the integration of signals from cytokine receptors and germline-encoded inhibitory receptors. cells undergo two distinct phases during murine CMV (MCMV) infection: a nonselective phase mediated proinflammatory cytokines specific driven signaling through Ly49H, an receptor that recognizes infected cells. We sought to delineate surface markers could distinguish had been activated nonselectively those specifically demonstrated stem Ag 1 (Sca-1) highly...
In recent years, CTLA-4 and PD-1/PD-L1 checkpoint inhibitors have proven to be effective become increasingly popular treatment options for metastatic melanoma other cancers. These agents work by enhancing autologous antitumor immune responses. Immune-related ophthalmologic complications been reported in association with inhibitor use but remain incompletely characterized. This study seeks investigate further characterize the neuro-ophthalmic ocular of blockade treatment.A survey was...
Purpose: Retinopathy of prematurity (ROP) is a leading cause childhood blindness. ROP occurs as consequence postnatal hyperoxia exposure in premature infants, resulting vasoproliferation the retina. The tetraspan protein epithelial membrane protein-2 (EMP2) highly expressed retinal pigment epithelium (RPE) adults, and it controls vascular endothelial growth factor (VEGF) production ARPE-19 cell line. We, therefore, hypothesized that Emp2 knockout (Emp2 KO) protects against neovascularization...
Purpose: Pathologic corneal neovascularization is a major cause of blindness worldwide, and treatment options are currently limited. VEGF one the critical mediators but current anti-VEGF therapies have produced limited results in cornea. Thus, additional therapeutic agents needed to enhance antiangiogenic arsenal. Our group previously demonstrated epithelial membrane protein-2 (EMP2) involvement pathologic angiogenesis multiple cancer models including breast glioblastoma. In this paper, we...
Purpose: Retinopathy of prematurity (ROP) results from postnatal hyperoxia exposure in premature infants and is characterized by aberrant neovascularization retinal blood vessels. Epithelial membrane protein-2 (EMP2) regulates hypoxia-inducible factor (HIF)–induced vascular endothelial growth (VEGF) production the ARPE-19 cell line genetic knock-out Emp2 a murine oxygen–induced retinopathy (OIR) model attenuates neovascularization. We hypothesize that EMP2 blockade via intravitreal injection...
Pathologic retinal neovascularization is a potentially blinding consequence seen in many common diseases including diabetic retinopathy, retinopathy of prematurity, and vaso-occlusive diseases. This study investigates epithelial membrane protein 2 (EMP2) its role as possible modulator angiogenesis human pigment epithelium (RPE) under hypoxic conditions. To effects, the RPE cell line ARPE-19 was genetically modified to either overexpress EMP2 or knock down levels, RNA sequencing western blot...
Murine models provide microvascular insights into the 3-D network disarray seen in retinopathy and cardiovascular diseases. Light-sheet fluorescence microscopy (LSFM) has emerged to capture retinal vasculature 3-D, allowing for assessment of progression potential screen new therapeutic targets mice. We hereby coupled LSFM, also known as selective plane illumination microscopy, with topological quantification, characterize vascular plexuses undergoing preferential obliteration.
Abstract NKG2D is a key component of cytotoxic antitumor and antiviral responses. Multiple viruses evade recognition by blocking ligand expression on infected cells. In contrast, cowpox virus targets directly encoding secreted antagonist, Orthopoxvirus MHC Class I-like Protein (OMCP). We have previously reported that OMCP also binds to the orphan receptor FcRL5 innate B Here, we demonstrate mammalian-derived, glycosylated but not FcRL5. Cowpox either lacking OMCP, or expressing an...
e15124 Background: The detailed study of ophthalmic immune-related adverse events (OirAEs) is integral importance in the evolving field cancer immunotherapy to inform management and treatment guidelines. This seeks quantify differences incidence recurrence rates OirAEs by immune checkpoint inhibitor (ICI) status a longitudinal big-data registry patients who are seen clinics. Methods: retrospective examined newly diagnosed with between January 1, 2013 December 31, 2017 American Academy...
ABSTRACT Murine models of visual impairment provide micro-vascular insights into the 3-D network disarray in retinopathy. Current imaging and analysis tend to be confined 2-D retinal vasculature. We hereby integrated selective plane illumination or known as light-sheet fluorescence microscopy (LSFM) with dual-illumination, followed by computational analyses, reveal topological vertical sprouts bridging primary secondary plexuses a postnatal mouse model oxygen-induced retinopathy (OIR)....