Background: In our previous study, we found that periostin was upregulated in prostate cancer, and its expression could be modulated by TGF-β. TGF-β upregulate some cells, both induce epithelial mesenchymal transition (EMT). We aimed to study the effect of process TGF-β-induced EMT cancer cells. Methods: constructed a lentivirus vector containing gene transduced it into PC3 DU145 After confirming overexpression PCR Western blotting, used an MTT assay establish growth curve measure cell...

Until recently, there have been limited options for patients with bone metastatic castration-resistant prostate cancer (BmCRPC) following the failure of or development resistance to docetaxel (DTX), which is one frontline treatments.Sterol regulatory element-binding protein 1 (SREBP1) reported regulate abnormal lipid metabolism and promote progression metastasis (PCa).The siRNA interferes SREBP1 may provide an efficient treatment when combined DTX.Methods: In this study, lipoic acid (LA)...

Combination immunotherapy is a promising strategy to remove the inhibitory effect of tumor microenvironment on immune effector cells, improving efficacy checkpoint inhibitor treatment in bladder cancer. However, it challenging deliver multiple drugs tissue effectively and simultaneously ensure optimal therapeutic effects. Macrophage-derived exosome-mimetic nanovesicles (EMVs) were designed validated as nanoplatform for coloading delivery CD73 (AB680) monoclonal antibody programmed cell death...

Background Predicting the recurrence and progression of Non-muscle-invasive bladder cancer(NMIBC) is critical for urologist. Histological grade provides significant prognostic information, especially prediction progression. Currently, 1973 2004 WHO classification co-exist. Which system better predicting rumor still a matter debate. Methodology/Principal Findings 348 patients diagnosed with Non-muscle invasive cancer were enrolled in our retrospective study. Paraffin sections assessed by an...

Proteomics may help us better understand the changes of multiple proteins involved in oncogenesis and progression prostate cancer(PCa) identify more diagnostic prognostic biomarkers. The aim this study was to screen biomarkers PCa by proteomics analysis using isobaric tags for relative absolute quantification(iTRAQ).The patients undergoing biopsies were classified into 3 groups according pathological results: benign hyperplasia (BPH, n = 20), PCa(n 20) BPH with local prostatic...

Abstract Background In our recent study, Periostin was up-regulated in prostate cancer(PCa) compared with benign hyperplasia (BPH) by proteomics analysis of biopsies. We investigated the effect sliencing RNA interference (RNAi) on proliferation and migration PCa LNCap cell line. Methods All biopsies from PCa, BPH local prostatic intraepithelial neoplasm(PIN) were analyzed iTRAQ(Isobaric tags for relative absolute quantification) technology. Western blotting immunohistochemical staining used...

Cancer-associated fibroblasts (CAFs) play a significant role in tumor development and treatment failure, yet the precise mechanisms underlying their contribution to renal cell carcinoma (RCC) remains underexplored. This study explored interaction between CAFs cells, related mechanisms. isolated from tissues promoted progression drugs resistance both

Epidemiological studies have assessed the association between kallikrein 3 (KLK3) polymorphisms and prostate cancer (PCa) susceptibility. However, published data on this are somewhat inconclusive.Articles investigating three KLK3 (rs1058205, rs2735839, rs266882) variants PCa susceptibility were searched from online databases, which included 35,838 patients 36,369 control participants. Odds ratios (ORs) with 95% confidence intervals (CIs) used to demonstrate strength of association. We also...

Objective: Chronic psychological stress is common in patients with bladder cancer. An increasing number of evidence demonstrated that psychiatric disorder leads to worse prognostic outcomes This study was investigate the effects chronic on growth cancer and its potential mechanisms. Methods: A xenograft mouse model established by subcutaneously implanting human cell line T24 into nude mice. All tumor-bearing mice (N=20) were randomly separated two groups. Mice control group subjected normal...

We aimed to explore whether chronic psychological stress affects the efficacy of immune checkpoint inhibitors (ICIs) immunotherapy in bladder cancer. The unpredictable mild (CUMS) process was applied during administration anti-PD-L1 for subcutaneous tumors mice. Tumor regression obviously shown therapy groups, while this effect notably attenuated by CUMS. Additionally, increased infiltration regulatory T-cells, decreased amount CD8+ lymphocytes, and reduced levels tumor-associated cytokines...

CX3CL1 is a chemokine that may play important roles in cancer immune regulation. Its mechanism bladder (BCa) poorly understood. The objective of the current study was to evaluate association between and BCa related biological mechanisms. A total 277 patients with were enrolled present study. expression disease outcome evaluated. In vitro vivo experiments performed using TCCSUP cell line investigate function BCa. Compared low expression, high significantly associated poorer progression-free...

Depression is a common mental disease in bladder cancer patients, leading to loss of happiness, an increase the suicide rate, and higher mortality. However, influence depression on tumor tissue remains unknown. In this current study, subcutaneous xenograft model was established male C57 mice with mouse cell line MB49. Chronic unpredictable mild stress (CUMS) simulate patients. The caused by CUMS confirmed testing sucrose preference plasma cortisol adrenocorticotropic hormone (ACTH) levels....

Prostate cancer (PCa) is the second most common malignancy in men, and androgen deprivation therapy (ADT) first-line therapy. However, cases will eventually develop into castration-resistant prostate (CRPC) after ADT treatment. Enzalutamide (Enz) a second-generation receptor inhibitor approved by Food Drug Administration to treat patients with CRPC. Unfortunately, receiving Enz treatment ultimately resistance via various complicated mechanisms. In this review, we introduce emerging...

Background: Patients with non-muscle-invasive bladder cancer (NMIBC) need accurate estimations of the risk recurrence and progression. Physicians can offer individualized therapy after identifying high-risk tumors. In our study, we compared applicability European Organization for Research Treatment Cancer (EORTC) tables American Urological Association (AUA) stratification in Chinese patients NMIBC. Methods: We retrospectively studied 301 NMIBC who underwent transurethral resection tumor...