A5087598968- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Cardiovascular Effects of Exercise
- Yersinia bacterium, plague, ectoparasites research
- Plant-based Medicinal Research
- Bacillus and Francisella bacterial research
- Cardiomyopathy and Myosin Studies
- ATP Synthase and ATPases Research
- Neonatal Respiratory Health Research
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Blood disorders and treatments
- Advanced Electron Microscopy Techniques and Applications
- Genetics, Aging, and Longevity in Model Organisms
- Infant Development and Preterm Care
- Autophagy in Disease and Therapy
- Neutropenia and Cancer Infections
- Renal Diseases and Glomerulopathies
- Pharmacological Effects of Natural Compounds
- Vitamin C and Antioxidants Research
- Retinoids in leukemia and cellular processes
- Pharmacological Effects and Assays
- Marine Biology and Environmental Chemistry
- Sex and Gender in Healthcare
Inner Mongolia Agricultural University
2023-2024
Institute of Microbiology
2023-2024
University of Wisconsin–Madison
2024
Kaiser Permanente
2024
Kaiser Permanente Santa Clara Medical Center
2024
Shaanxi University of Chinese Medicine
2023
University of Michigan
2012-2022
Chengdu Center for Disease Control and Prevention
2017-2022
First Affiliated Hospital of Zhengzhou University
2018-2022
Peking University
2009-2020
Plakophilin-2 (PKP2) is a component of the desmosome and known for its role in cell-cell adhesion. Mutations human PKP2 associate with life-threatening arrhythmogenic cardiomyopathy, often right ventricular predominance. Here, we use range state-of-the-art methods cardiomyocyte-specific, tamoxifen-activated, knockout mouse to demonstrate that addition cell adhesion, necessary maintain transcription genes control intracellular calcium cycling. Lack reduces expression Ryr2 (coding Ryanodine...
Significance Catecholaminergic polymorphic ventricular tachycardia (CPVT) is an arrhythmogenic syndrome characterized by life-threatening cardiac arrhythmias triggered physical exercise or emotional stress. Although patients with CPVT have no abnormalities in structure, they present fibrillation sudden death as the first symptom. Most cases are linked to mutations ryanodine receptor (RyR2), intracellular Ca 2+ channel that provides majority of enables heart contraction. The current mechanism...
Exchanges of matrix contents are essential to the maintenance mitochondria. Cardiac mitochondrial exchange content in two ways: by direct contact with neighboring mitochondria and over longer distances. The latter mode is supported thin tubular protrusions, called nanotunnels, that other at relatively long Here, we report cardiac myocytes heterozygous mice carrying a catecholaminergic polymorphic ventricular tachycardia-linked RyR2 mutation (A4860G) show unique unusual response:...
As the most prototypical G protein-coupled receptor, beta-adrenergic receptor (betaAR) regulates pace and strength of heart beating by enhancing synchronizing L-type channel (LCC) Ca(2+) influx, which in turn elicits greater sarcoplasmic reticulum (SR) release flux via ryanodine receptors (RyRs). However, whether how betaAR-protein kinase A (PKA) signaling directly modulates RyR function remains elusive highly controversial. By using unique single-channel imaging technology, we measured...
Calstabin2 is a component of the cardiac ryanodine receptor (RyR2) macromolecular complex, which modulates Ca(2+) release from sarcoplasmic reticulum in cardiomyocytes. Previous reports implied that genetic deletion leads to phenotypes related aging. However, mechanistic role process aging remains unclear. To assess whether involved age-related heart dysfunction, we studied knockout (KO) and control wild-type (WT) mice. We found significant association between Indeed, aged KO mice exhibited...
Abstract Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) allow investigations in a human cardiac model system, but disorganized mechanics and immaturity of hPSC-CMs on standard two-dimensional surfaces have been hurdles. Here, we developed platform micron-scale muscle bundles to control biomechanics arrays thousands purified, independently contracting strips elastomer substrates with far greater throughput than single cell methods. By defining geometry workload this...
Hypertrophic cardiomyopathy (HCM) is triggered mainly by mutations in genes encoding sarcomeric proteins, but a significant proportion of patients lack genetic diagnosis. We identified novel mutation the ryanodine receptor 2, RyR2-P1124L, patient from genotype-negative HCM cohort. The aim this study was to determine whether RyR2-P1124L triggers functional and structural alterations isolated RyR2 channels whole hearts. found that P1124L induces conformational changes SPRY2 domain RyR2....
Background Plague, caused by the bacterium Yersinia pestis , is a zoonotic disease that poses considerable threats to human health. Nucleic acid tests are crucial for plague surveillance and rapid detection of Y . However, inhibitors in complex samples such as soil animal tissues often hamper nucleic detection, leading reduced rate identifying low concentrations To address this challenge, we developed sensitive specific droplet digital polymerase chain reaction (ddPCR) assay detecting DNA...
Loss-of-function (LOF) variants in SCN1B, encoding voltage-gated sodium channel β1 subunits, are linked to human diseases with high risk of sudden death, including developmental and epileptic encephalopathy cardiac arrhythmia. Subunits modulate the cell-surface localization, gating, kinetics pore-forming α subunits. They also participate cell-cell cell-matrix adhesion, resulting intracellular signal transduction, promotion cell migration, calcium handling, regulation morphology. Here, we...
Objectives: To examine the association between smoking cessation and risk of type 2 diabetes with emphasis on post-cessation weight gain. Methods: In total, 8,951 participants from China Health Retirement Longitudinal Study at baseline (2011) were included. Diabetes incidence was accessed third survey (2015). Current smokers treated as reference odds ratios (OR) for never smokers, recent, long-term quitters computed using multivariable logistic regression. Stratified analysis further...
Sorcin, a penta-EF hand Ca2+-binding protein expressed in cardiomyocytes, is known to interact with ryanodine receptors and other Ca2+ regulatory proteins. To investigate sorcin's influence on cardiac excitation-contraction coupling its role the development of malfunctions, we generated sorcin knockout (KO) mouse model. Sorcin KO mice presented ventricular arrhythmia sudden death when challenged by acute stress induced isoproterenol plus caffeine. Chronic stress, which was transverse aortic...
The heart contraction is controlled by the Ca2+-induced Ca2+ release (CICR) between L-type channels and ryanodine receptors (RyRs). FK506-binding protein FKBP12.6 binds to RyR subunits, but its role in stabilizing function has been debated for long. Recent reports of high-resolution structure show that HD2 domain SPRY2 neighbouring subunit FKBP-bound RyR1 detached invisible FKBP-null RyR2. present study was test consequence absence on situ activation RyR2.Using whole-cell patch-clamp...
Background Type VI secretion system (T6SS) is widely present in Gram-negative bacteria and directly mediates antagonistic prokaryote interactions. PAAR (proline-alanine-alanine-arginine repeats) proteins have been proven essential for T6SS-mediated target cell killing. Although are commonly found A. baumannii , their biological functions not fully disclosed yet. In this study, we investigated the of a protein termed TagP (T6SS-associated-gene PAAR), encoded by gene ACX60_RS09070 outside core...
Abstract During exercise or stress, the sympathetic system stimulates cardiac contractility via β‐adrenergic receptor (β‐AR) activation, resulting in phosphorylation of ryanodine (RyR2). Three RyR2 sites have taken prominence excitation–contraction coupling: S2808 and S2030 are described as protein kinase A specific S2814 a Ca 2+ /calmodulin type‐2‐specific site. To examine contribution these phosphosites to signalling, we generated double knock‐in (DKI) mice which Ser2808 Ser2814 both been...
Abstract Background Fushenmu ( Pini Radix in Poria , FSM) is a folk parasitic herb that has been mainly used for palpitation and amnesiain traditional Chinese medicine (TCM). Recently, as an individual or component of formulations, exhibits therapeutic potential the treatment cardiac arrhythmias. Yet, how specific targets pathways inhibit arrhythmia not yet reported. Methods Here, based on clinical functional genomics, metabolomics molecular biologic technologies, network construction...
Pediatric acute promyelocytic leukemia (APL) accounts for 10% of pediatric myelogenous (AML) case and is accompanied by a tendency to hemorrhage. miR-188-5p plays an important role in adult AML. Therefore, the purpose this study was explore effects on cell proliferation apoptosis tumor growth, its mechanism APL patients.Survival-associated miRNAs or mRNAs from TCGA database associated with AML were identified via using "survival R" package R language. CCK8, clone formation, flow cytometry,...
In the early life of preterm infants, relationship between heart function and length hospitalization is unclear. This study aims to examine association serum NT-proBNP level on 7th day (NT-proBNP7) after birth among infants.A retrospective cohort was conducted. Patients included 709 infants born at 28-31 weeks' gestational age (GA) admitted NICU First Affiliated Hospital Zhengzhou University December 20, 2016, April 31, 2021. Main outcome: Late discharge (postmenstrual in fourth quartile...
Abstract Aims β-adrenergic receptors (βARs) play pivotal roles in regulating cardiac excitation–contraction (E-C) coupling. Global signalling of β1ARs up-regulates both the influx Ca2+ through sarcolemmal L-type channels (LCCs) and release from sarcoplasmic reticulum (SR) ryanodine (RyRs). However, we recently found that β2AR stimulation meditates ‘offside compartmentalization’, confining β1AR into subsarcolemmal nanodomains without reaching SR proteins. In present study, aim to investigate...