A5046516052- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Tendon Structure and Treatment
- Electrocatalysts for Energy Conversion
- Supercapacitor Materials and Fabrication
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Occupational and environmental lung diseases
- Extraction and Separation Processes
- Graphene research and applications
- Wound Healing and Treatments
- Heterotopic Ossification and Related Conditions
- Adsorption and biosorption for pollutant removal
- Advancements in Battery Materials
- Angiogenesis and VEGF in Cancer
- Nanoporous metals and alloys
- Advanced Materials and Mechanics
- Inhalation and Respiratory Drug Delivery
- Advanced Battery Materials and Technologies
- Optical Coherence Tomography Applications
- Systemic Sclerosis and Related Diseases
- Cardiomyopathy and Myosin Studies
- Graphite, nuclear technology, radiation studies
University of Michigan
2019-2023
University at Buffalo, State University of New York
2015
Fibrosis, characterized by aberrant tissue scarring from activated myofibroblasts, is often untreatable. Although the extracellular matrix becomes increasingly stiff and fibrous during disease progression, how these physical cues affect myofibroblast differentiation in 3D poorly understood. Here, we describe a multicomponent hydrogel that recapitulates structure of interstitial regions where idiopathic pulmonary fibrosis (IPF) initiates. In contrast to findings on 2D hydrogels, was inversely...
Cellular phenotype is heavily influenced by the extracellular matrix (ECM), a complex and tissue-specific three-dimensional structure with distinct biophysical biochemical properties. As naturally derived cell culture platforms are difficult to controllably modulate, engineered synthetic ECMs have facilitated our understanding of how specific properties direct behavior. However, approaches typically lack fibrous topography, hallmark stromal interstitial in vivo. To construct tunable...
Cells sense the extracellular environment and mechanical stimuli translate these signals into intracellular responses through mechanotransduction, which alters cell maintenance, proliferation, differentiation. Here we use a mouse model of trauma-induced heterotopic ossification (HO) to examine how cell-extrinsic forces impact mesenchymal progenitor (MPC) fate. After injury, single-cell (sc) RNA sequencing injury site reveals an early increase in MPC genes associated with pathways adhesion...
Synthetic matrices which mimic the extracellular composition of native tissue create a comprehensive model for studying development and disease. Here, we have engineered composite material retains cell-secreted ECM culture ovarian follicles by embedding electrospun dextran fibers functionalized with basement membrane binder (BMB) peptide in PEG hydrogels. In presence ECM-sequestering fibers, encapsulated immature primordial stromal cells aggregated into large organoid-like structures dense...
Abstract Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) allow investigations in a human cardiac model system, but disorganized mechanics and immaturity of hPSC-CMs on standard two-dimensional surfaces have been hurdles. Here, we developed platform micron-scale muscle bundles to control biomechanics arrays thousands purified, independently contracting strips elastomer substrates with far greater throughput than single cell methods. By defining geometry workload this...
Fibrous extracellular matrix (ECM) proteins provide mechanical structure and adhesive scaffolding to resident cells within stromal tissues. Aligned ECM fibers play an important role in directing morphogenetic processes, supporting loads, facilitating cell migration. Various methods have been developed align purified biopolymer hydrogels, such as type I collagen, including flow-induced alignment, uniaxial tensile deformation, magnetic particles. However, biopolymers limited orthogonal...
Synthetic hydrogels represent an exciting avenue in the field of regenerative biomaterials given their injectability, orthogonally tunable mechanical properties, and potential for modular inclusion cellular cues. Separately, recent advances soluble factor release technology have facilitated control over milieu cell microenvironments via microparticles. A composite hydrogel incorporating both these components can robustly mediate tendon healing following a single injection. Here, synthetic...
ABSTRACT Fibrosis is central to numerous fatal conditions including solid cancers, pulmonary fibrosis, cirrhosis and post-infarct cardiac fibrosis amongst many others, thereby collectively contributing 45% of all deaths in developed nations. The potential for across most organ systems may stem from its connections wound healing the ubiquitous presence vascular endothelium. Endothelial cells (ECs) angiogenesis, associated biological program healing, have been heavily implicated organ-specific...
Emerging cell-based therapies such as stem cell therapy and immunotherapy have attracted broad attention in both biological research clinical practice. However, a long-standing technical gap of is the difficulty directly assessing treatment efficacy via tracking therapeutically administered cells. Therefore, imaging techniques to follow
ABSTRACT The peritumoral stroma is a complex 3D tissue that provides cells with myriad biophysical and biochemical cues. Histologic observations suggest during metastatic spread of carcinomas, these cues influence transformed epithelial cells, prompting diversity migration modes spanning single cell multicellular phenotypes. Purported consequences variations in tumor escape strategies include differential capability therapy resistance. Therefore, understanding how from the stromal...
Abstract Fibrosis is often untreatable and characterized by aberrant tissue scarring from activated myofibroblasts. Although the extracellular matrix becomes increasingly stiff fibrous during disease progression, how these physical cues impact myofibroblast differentiation in 3D poorly understood. Here we describe a multicomponent hydrogel that recapitulates structure hallmark to interstitial regions where idiopathic pulmonary fibrosis (IPF) initiates. In contrast findings on 2D hydrogels,...
The peritumoral stroma is a complex 3D tissue that provides cells with myriad biophysical and biochemical cues. Histologic observations suggest during metastatic spread of carcinomas, these cues influence transformed epithelial cells, prompting diversity migration modes spanning single cell multicellular phenotypes. Purported consequences variations in tumor escape strategies include differential capability therapy resistance. Therefore, understanding how from the stromal microenvironment...