A5089735895- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Nuclear Structure and Function
- Cell Adhesion Molecules Research
- Platelet Disorders and Treatments
- Erythrocyte Function and Pathophysiology
- Acute Myeloid Leukemia Research
- Tissue Engineering and Regenerative Medicine
- Hematopoietic Stem Cell Transplantation
- Cancer Cells and Metastasis
- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Blood properties and coagulation
- Korean Urban and Social Studies
- Hippo pathway signaling and YAP/TAZ
- Microfluidic and Bio-sensing Technologies
- Blood disorders and treatments
- RNA Interference and Gene Delivery
- Consumer Perception and Purchasing Behavior
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Amino Acid Enzymes and Metabolism
- Pluripotent Stem Cells Research
- Hemoglobinopathies and Related Disorders
- Skin and Cellular Biology Research
University of Illinois Chicago
2016-2025
Chung-Ang University
2024-2025
Center for Vascular Biology Research
2023-2024
Seoul National University
2023-2024
University of Illinois Urbana-Champaign
2016-2022
Illinois College
2016-2022
UNSW Sydney
2021
EMBL Australia
2021
University of Pennsylvania
2010-2020
Chungnam National University
2015-2018
The mechanical properties of the microenvironment and direct contact-mediated cell-cell interactions are two variables known to be important in determination stem cell differentiation fate, but little is about interplay these cues. Here, we use a micropatterning approach on polyacrylamide gels tunable stiffnesses study how homotypic contacts stiffness affect different stages osteogenesis mesenchymal cells (MSCs). Nuclear localization transcription factors associated with depended substrate...
Significance Mesenchymal stem cell (MSC) therapies have shown strong preclinical promise, particularly in ameliorating diseases of immune dysregulation. However, these results largely failed to demonstrate efficacy clinical settings, likely due large dosages necessitated by the short residence time infused cells, suboptimal phenotype, and rejection. We that a microfluidic-based biomaterial encapsulation approach can increase vivo after intravenous (i.v.) delivery more than an order...
Hematopoietic stem and progenitor cells, as well nucleated erythroblasts megakaryocytes, reside preferentially in adult marrow microenvironments whereas other blood cells readily cross the endothelial barrier into circulation. Because nucleus is largest organelle we hypothesized that (i) cell sorting across microporous barriers regulated by nuclear deformability controlled lamin-A -B, (ii) lamin levels directly modulate hematopoietic programs. Mass spectrometry-calibrated intracellular flow...
Significance Most chemotherapy drugs treat, but do not cure, cancer patients due to resistance. New high-throughput screening assays are emerging better predict drug efficacy by recapitulating tumor microenvironments with three-dimensional hydrogels. Our platform exploits the mechanical tunability of alginate hydrogels introduce biophysical cues into assays. The utility this approach is demonstrated findings unique modes growth modulation matrix stiffness and mechanosensitivity actions in...
Matrix stiffness that is sensed by a cell or measured purely physical probe reflects the intrinsic elasticity of matrix and also how thick thin is. Here, mesenchymal stem cells (MSCs) their nuclei spread in response to thickness-corrected microelasticity, with increases nuclear tension resulting from myosin-II lamin-A,C. Linearity between widely varying projected area its nucleus across many matrices, timescales, activity levels indicates constant ratio nucleus-to-cell volume, despite MSCs'...
Soft extracellular matrix boosts the activation of MSCs by TNFα to generate and recruit monocytes in a paracrine manner.
The pathogenesis of lung fibrosis involves hyperactivation innate and adaptive immune pathways that release inflammatory cytokines growth factors such as tumor factor (TGF)β1 induce aberrant extracellular matrix protein production. During the genesis pulmonary fibrosis, resident alveolar macrophages are replaced by a population newly arrived monocyte-derived interstitial subsequently transition into (Mo-AMs). These transitioning cells initiate releasing profibrotic remodeling matrix. Here,...
Neuromorphic olfactory systems have been actively studied in recent years owing to their considerable potential electronic noses, robotics, and neuromorphic data processing systems. However, conventional gas sensors typically the ability detect hazardous levels but lack synaptic functions such as memory recognition of accumulation, which are essential for realizing human-like sensory system. In this study, a seamless architecture system capable detecting memorizing present level accumulation...
We report the development of polyacrylamide hydrogels with photoswitchable stiffness using solely visible light and their application to cell culture. have previously shown that azobenzenes can control binding constants dynamic covalent boronic ester bonds (Chem. Sci. 2018, 9, 5987; J. Am. Chem. Soc. 2020, 42, 19969). Here we show these be incorporated into are stable for at least 10 days in buffer without changes or photoresponse. Reversible stiffening softening achieved green blue...
ABSTRACT The macrophage checkpoint receptor SIRPα signals against phagocytosis by binding CD47 expressed on all cells – including macrophages. Here, we found that inhibiting cis interactions between and the same increased engulfment (‘eating’) approximately level as trans interactions. Antibody blockade of CD47, pursued in clinical trials cancer, was applied separately to human-derived macrophages red blood cell (RBC) targets for phagocytosis, both scenarios produced surprisingly similar...
Vascular endothelial (VE)–cadherin forms homotypic adherens junctions (AJs) in the endothelium, whereas N-cadherin heterotypic adhesion between cells and surrounding vascular smooth muscle pericytes. Here we addressed question whether both cadherin complexes communicate through intracellular signaling contribute to integrity of barrier. We demonstrated that deletion (Cdh2) either or pericytes increases junctional permeability lung brain secondary reduced accumulation VE-cadherin at AJs....
Extracellular vesicles (EVs) are cell-secreted particles with broad potential to treat tissue injuries by delivering cargo program target cells. However, improving the yield of functional EVs on a per cell basis remains challenging due an incomplete understanding how microenvironmental cues regulate EV secretion at nanoscale. We show that mesenchymal stromal cells (MSCs) seeded engineered hydrogels mimic elasticity soft tissues lower integrin ligand density secrete ∼10-fold more than MSCs...
Conjugated linoleic acid (CLA) has been reported to inhibit mouse skin carcinogenesis, particularly in the promotion stage, but underlying molecular mechanisms remain poorly understood. Since persistent induction of cyclooxygenase-2 (COX-2) is frequently implicated we investigated effect cis -9, trans -11-CLA (9Z,11E-CLA) on tumor promoter-induced COX-2 expression HR-1 hairless vivo . Topical application 9Z,11E-CLA caused significant inhibition at 6 h induced by 10 nmol 12- O...
Synthetic hydrogels represent an exciting avenue in the field of regenerative biomaterials given their injectability, orthogonally tunable mechanical properties, and potential for modular inclusion cellular cues. Separately, recent advances soluble factor release technology have facilitated control over milieu cell microenvironments via microparticles. A composite hydrogel incorporating both these components can robustly mediate tendon healing following a single injection. Here, synthetic...
Abstract Conventional gas sensors typically focus on detecting transient gases with critical concentrations but lack the ability to detect hazards resulting from cumulative exposure. Here, study demonstrates a dual‐mode nitrogen dioxide (NO 2 ) sensor utilizing carbon nanotube thin‐film transistors, which features detection mode for sensitive of inflow and accumulation monitoring exposure, offering efficient compact analysis both immediate prolonged NO The proposed is capable through charge...