A5051588276- Prostate Cancer Treatment and Research
- Cancer, Lipids, and Metabolism
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Sperm and Testicular Function
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Microfluidic and Bio-sensing Technologies
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- Neurobiology and Insect Physiology Research
- Angiogenesis and VEGF in Cancer
- Anesthesia and Pain Management
- Prenatal Screening and Diagnostics
- AI in cancer detection
- Genomic variations and chromosomal abnormalities
- Hemostasis and retained surgical items
- Genetics, Aging, and Longevity in Model Organisms
- Vascular Malformations Diagnosis and Treatment
- Prostate Cancer Diagnosis and Treatment
- Ovarian cancer diagnosis and treatment
- Dialysis and Renal Disease Management
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Zebrafish Biomedical Research Applications
University of North Carolina at Chapel Hill
2020-2024
North Carolina State University
2020-2024
Sinai Hospital
2024
Canadian Apheresis Group
2024
University of Minnesota
2024
Personalis (United States)
2023
New York University
2022-2023
University of California, Los Angeles
2021
Loyola Marymount University
2021
Banaras Hindu University
2021
Abstract Prostate cancer is clinically heterogeneous, ranging from indolent to lethal disease. Expression profiling previously defined three subtypes of prostate cancer, one (subtype-1) linked favorable behavior, and the others (subtypes-2 -3) with a more aggressive form To explore disease heterogeneity at genomic level, we carried out array-based comparative hybridization (array CGH) on 64 tumor specimens, including 55 primary tumors 9 pelvic lymph node metastases. Unsupervised cluster...
<b><i>Objective:</i></b> To develop a microarray-based method for noninvasive prenatal testing (NIPT) and compare it with next-generation sequencing. <b><i>Methods:</i></b> Maternal plasma from 878 pregnant women, including 187 trisomy cases (18 13, 37 18, 132 21), was evaluated risk. Targeted chromosomes were analyzed using Digital Analysis of Selected Regions (DANSR™) assays. DANSR products subsequently divided between two DNA quantification...
Traumatic non-compressible hemorrhage is a leading cause of civilian and military mortality its treatment requires massive transfusion blood components, especially platelets. However, in austere battlefield locations, access to platelets highly challenging due limited supply portability, high risk bacterial contamination short shelf-life. To resolve this, we have developed an I.V.-administrable 'synthetic platelet' nanoconstruct (SynthoPlate), that can mimic amplify body's natural hemostatic...
The mutational activation of oncogenes drives cancer development and progression. Classic oncogenes, such as MYC RAS , are active across many different types. In contrast, “lineage-survival” represent a distinct emerging class typically comprising transcriptional regulators specific cell lineage that, when deregulated, support the proliferation survival cancers derived from that lineage. Here, in large collection colorectal lines tumors, we identify recurrent amplification chromosome 13, an...
Cells are the singular building blocks of life, and a comprehensive understanding morphology, among other properties, is crucial to assessment underlying heterogeneity. We developed Computational Sorting Mapping Single (COSMOS), platform based on Artificial Intelligence (AI) microfluidics characterize sort single cells real-time deep learning interpretation high-resolution brightfield images. Supervised models were applied cell lines dissociated primary tissue high-dimensional embedding...
Across the nervous system, neurons with similar attributes are topographically organized. This topography reflects developmental pressures. Oddly, vestibular (balance) nuclei thought to be disorganized. By measuring activity in birthdated neurons, we revealed a functional map within central projection nucleus that stabilizes gaze larval zebrafish. We first discovered both somatic position and stimulus selectivity follow neuron birthdate. Next, electron microscopy loss-of-function assays,...
Somatic activating mutations of
To assess the performance of a directed chromosomal analysis approach in prenatal evaluation fetal sex chromosome aneuploidy.We analyzed 432 frozen maternal plasma samples obtained from patients prior to undergoing diagnostic testing. The cohort included women greater than 18 years age with singleton pregnancy 10 weeks gestation. Samples were using chromosome-selective (DANSR(TM) ) and risk algorithm that incorporates fraction (FORTE(TM) ).The 34 cases aneuploidy. assay correctly identified...
Clear cell carcinoma (CCC) is a histologically distinct subtype that arises in several organ systems and marked by cytoplasmic clearing, attributed to abundant intracellular glycogen. Previously, transcription factor hepatocyte nuclear 1-beta (HNF1B) was identified as biomarker of ovarian CCC. Here, we set out explore more broadly the relation between HNF1B carcinomas with clear histology. expression, evaluated immunohistochemistry, significantly associated histology across diverse...
2-Substituted 1,3-benzoxazines having a phenyl group as an oxazine ring substituent are synthesized with benzaldehyde through 2-hydroxy-<italic>N</italic>-phenylbenzylamine structures.
Mechanical forces regulate a diverse set of biological processes at cellular, tissue, and organismal length scales. Investigating the cellular molecular mechanisms that underlie conversion mechanical to responses is challenged by limitations traditional animal models in vitro cell culture, including poor control over applied force highly artificial culture environments. Recent advances fabrication methods material processing have enabled development microfluidic platforms provide precise...
Children who are too large for harness restraints but small to obtain good restraint from a vehicle seatbelt alone should be seated in belt-positioning booster.Boosters have been shown significantly reduce abdominal injuries caused by seatbelts.This effectiveness may due part the fact that boosters effective seat cushion length, allowing children sit more comfortably without slouching.NHTSA recommends do not use until they at least 145 cm tall.In this paper, data several sources were...
Efficient delivery of oxygen and nutrients to tissues requires an intricate balance blood, lymphatic, interstitial fluid pressures (IFPs), gradients in pressure drive the flow lymph, through tissues. While specific mechanical stimuli, such as wall shear stress, have been shown modulate cellular signaling pathways along with gene protein expression patterns, understanding key signals imparted by flowing how these are integrated across multiple cells cell types native is incomplete due...
Abstract Cardiovascular disease is the cause of death in ≈50% hemodialysis patients. Accumulation uremic solutes systemic circulation thought to be a key driver endothelial dysfunction that underlies elevated cardiovascular events. A challenge understanding mechanisms relating chronic kidney lack vitro models allow screening effects environment on endothelium. Here, method described for microfabrication human blood vessels from donor cells and perfused with serum. The resulting donor‐derived...
Abstract A child’s success in school relies on their ability to quickly grasp language and reading skills, the foundations of which are acquired even before entering a formal classroom setting. Previous studies preschoolers have begun establish relationships linking beat synchronization, preliteracy auditory processing. Beat synchronization involves integration sensorimotor systems with cognitive circuits and, therefore calls many same neural networks as language. Using drumming task, we...
Extravasation of circulating cells is an essential process that governs tissue inflammation and the body's response to pathogenic infection. To initiate anti-inflammatory phagocytic functions within tissues, immune must cross vascular endothelial barrier from vessel lumen subluminal extracellular matrix. In this work, we present a microfluidic approach enables recreation three-dimensional, perfused formed by human embedded collagen-rich Monocytes are introduced into perfusate, investigate...
The ease of getting into and out passenger cars light trucks is a critical component customer acceptance product differentiation. In commercial vehicles, the health safety drivers affected by design steps handholds they use to get cab. Ingress/egress assessment appears represent substantial application opportunity for digital human models. complexity space range possible biomechanical subjective measures interest mean that developing useful empirical models difficult, requiring large-scale...