A5078746937- Neurobiology and Insect Physiology Research
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Olfactory and Sensory Function Studies
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Insect and Arachnid Ecology and Behavior
- Seismic Imaging and Inversion Techniques
- Optical Coherence Tomography Applications
- Optical Imaging and Spectroscopy Techniques
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Animal Behavior and Reproduction
- Cellular Mechanics and Interactions
- Geological Modeling and Analysis
- Seismology and Earthquake Studies
- Zebrafish Biomedical Research Applications
- Retinal Development and Disorders
- Glioma Diagnosis and Treatment
- Advanced Electron Microscopy Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Blood properties and coagulation
- Cancer Mechanisms and Therapy
- Physiological and biochemical adaptations
- Pickering emulsions and particle stabilization
Columbia University
2015-2024
Brain (Germany)
2020-2024
University of Kentucky HealthCare
2022
Optica
2017-2019
Massachusetts General Hospital
2015
Boston University
2014
What are the spatial and temporal scales of brainwide neuronal activity? We used swept, confocally-aligned planar excitation (SCAPE) microscopy to image all cells in a large volume brain adult Drosophila with high spatiotemporal resolution while flies engaged variety spontaneous behaviors. This revealed neural representations behavior on multiple scales. The activity most neurons correlated (or anticorrelated) running flailing over timescales that ranged from seconds minute. Grooming...
Across the nervous system, neurons with similar attributes are topographically organized. This topography reflects developmental pressures. Oddly, vestibular (balance) nuclei thought to be disorganized. By measuring activity in birthdated neurons, we revealed a functional map within central projection nucleus that stabilizes gaze larval zebrafish. We first discovered both somatic position and stimulus selectivity follow neuron birthdate. Next, electron microscopy loss-of-function assays,...
What are the spatial and temporal scales of brainwide neuronal activity, how do activities at different interact? We used SCAPE microscopy to image a large fraction central brain adult Drosophila melanogaster with high spatiotemporal resolution while flies engaged in variety behaviors, including running, grooming flailing. This revealed neural representations behavior on multiple scales. The activity most neurons across correlated (or, some cases, anticorrelated) running flailing over...
Learning alters cortical representations and improves perception. Apical tuft dendrites in layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons barrel cortex as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not repeated...
SUMMARY Learning alters cortical representations and improves perception. Apical tuft dendrites in Layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons barrel cortex as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not...
Abstract Diffusely infiltrating gliomas are known to cause alterations in cortical function, vascular disruption and seizures. These neurological complications present major clinical challenges, yet their underlying mechanisms causal relationships disease progression poorly characterized. Here, we followed glioma awake Thy1-GCaMP6f mice using in-vivo wide-field optical mapping monitor both neuronal activity functional hemodynamics. The bilateral synchrony of spontaneous glioma-infiltrated...
ABSTRACT Across the nervous system, neurons with similar attributes are topographically organized. This topography reflects developmental pressures. Oddly, vestibular (balance) nuclei thought to be disorganized. By measuring activity in birthdated neurons, we revealed a functional map within central projection nucleus that stabilizes gaze larval zebrafish. We first discovered both somatic position and stimulus selectivity follow neuron birthdate. Next, electron microscopy loss-of-function...
Learning alters cortical representations and improves perception. Apical tuft dendrites in Layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not repeated stimulus...
Learning alters cortical representations and improves perception. Apical tuft dendrites in Layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons barrel cortex as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not repeated...
Learning alters cortical representations and improves perception. Apical tuft dendrites in layer 1, which are unique their connectivity biophysical properties, may be a key site of learning-induced plasticity. We used both two-photon SCAPE microscopy to longitudinally track tuft-wide calcium spikes apical 5 pyramidal neurons barrel cortex as mice learned tactile behavior. Mice were trained discriminate two orthogonal directions whisker stimulation. Reinforcement learning, but not repeated...
Get PDF Email Share with Facebook Tweet This Post on reddit LinkedIn Add to CiteULike Mendeley BibSonomy Citation Copy Text H. Yu, P. T. Galwaduge, V. Voleti, K. B. Patel, M. A. Shaik, W. Li, and E. C. Hillman, "Combining Near-infrared Excitation Swept Confocally-aligned Planar (SCAPE) Microscopy for Fast, Volumetric Imaging in Mouse Brain," Biophotonics Congress: Biomedical Optics Congress 2018 (Microscopy/Translational/Brain/OTS), OSA Technical Digest (Optica Publishing Group, 2018), paper...
Abstract Multiphase suspensions are complex systems where microscopic interactions between suspended bubbles, particles, and liquids can significantly alter bulk behavior. Observing the internal mechanics of such help constrain dynamics natural multiphase flows. To capture these processes at high speed in three dimensions, we propose use Swept Confocally Aligned Planar Excitation (SCAPE) microscopy analog experiments. This imaging technique, developed for neuroscience biology, uses a sweeping...
SCAPE microscopy allows real-time 3D visualization of intact human tissues at cellular resolution using autofluorescence or topical/intravenous dyes. We are developing a portable clinical system suitable for open/laparoscopic surgery and surveillance accessible tissues.
Freshly excised tissues were imaged with SCAPE microscopy, allowing rapid 3D visualization. Results suggest in situ imaging using could be used to diagnose and monitor diseased tissue, guide targeted biopsies surgical resection.