A5056535790- Traumatic Brain Injury and Neurovascular Disturbances
- Advanced Fluorescence Microscopy Techniques
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Functional Brain Connectivity Studies
- Neonatal and fetal brain pathology
- Optical Imaging and Spectroscopy Techniques
- Advanced MRI Techniques and Applications
- Respiratory Support and Mechanisms
- Proteoglycans and glycosaminoglycans research
- Cardiac Arrest and Resuscitation
- Photoacoustic and Ultrasonic Imaging
- Glioma Diagnosis and Treatment
- Date Palm Research Studies
- Sodium Intake and Health
- Nitric Oxide and Endothelin Effects
- Automotive and Human Injury Biomechanics
- Neonatal Respiratory Health Research
- Cardiovascular Health and Disease Prevention
- Thermal Regulation in Medicine
- SARS-CoV-2 detection and testing
- Advanced Glycation End Products research
- COVID-19 diagnosis using AI
- Mitochondrial Function and Pathology
- Technology and Data Analysis
Children's Hospital of Pittsburgh
2024-2025
Columbia University
2011-2024
Optica
2015-2024
Children's Hospital of Philadelphia
2020-2021
Google (United States)
2017
Allen Institute for Brain Science
2016
Cleveland Clinic
2011
Significance Resting-state functional connectivity mapping exploits correlations in the magnetic resonance imaging (fMRI) blood oxygen level-dependent (BOLD) signal across brain. However, results are difficult to interpret without an understanding of neural correlates these hemodynamic fluctuations. This work uses mice which activity and brain hemodynamics can be mapped simultaneously. We show that resting-state predicted from spontaneous correspond a series driven increases local volume,...
The functional modulation of blood flow in the brain is critical for health and basis contrast magnetic resonance imaging. There evident coupling between increases neuronal activity local flow; however, many aspects this neurovascular remain unexplained by current models. Based on rapid dilation distant pial arteries during cortical hyperemia, we hypothesized that endothelial signaling may play a key role long-range propagation vasodilation hyperemia brain. Although well characterized...
Background A combination of horse anti-thymocyte globulin and cyclosporine produces responses in 60–70% patients with severe aplastic anemia. We performed a phase II study rabbit as first-line therapy for anemia.Design Methods Twenty anemia treated were compared to 67 historical control cases matched clinical characteristics globulin.Results Response rates at 3, 6 12 months similar or globulin: 40% versus 55% (P=0.43), 45% 58% (P=0.44) 50% (P=0.61), respectively. No differences early...
In the adult brain, increases in neural activity lead to local blood flow. However, many prior measurements of functional hemodynamics neonatal including magnetic resonance imaging (fMRI) human infants, have noted altered and even inverted hemodynamic responses stimuli. Here, we demonstrate that localized early postnatal mice does not evoke flow as elucidate metabolic correlates these a function developmental age. Using wide-field GCaMP imaging, development somatosensory stimulus is...
Cortical spreading depolarizations (CSDs) are increasingly suspected to play an exacerbating role in a range of acute brain injuries, including stroke, possibly through their interactions with cortical blood flow. We use simultaneous wide-field imaging neural activity and hemodynamics Thy1-GCaMP6f mice explore the neurovascular dynamics CSDs during following Rose Bengal-mediated photothrombosis. observed all as slow-moving waves GCaMP fluorescence extending far beyond photothrombotic area....
Amazon Web Services, or AWS, is an easy-touse, flexible, and reasonably priced cloud platform. Many Services (AWS) customers use RDBMS, relational database management systems. Deploying setting up systems made easier with Oracle Database on AWS. Relational Service (RDS) by allows users to manage databases. These challenges are distilled into a Level Agreement (SLA) that specifies the standards for quality of service provided tenants. In addition, SLA needs think about how renters' irregular...
Cerebral oedema or brain tissue swelling is a significant complication following traumatic injury stroke that can increase the intracranial pressure (ICP) and impair blood flow. Here, we have identified potential driver of oedema: negatively charged molecules fixed within cells. This charge density (FCD), once exposed, could ICP through Donnan effect. We shown metabolic processes membrane integrity are required for concealing this FCD as slices rat cortex swelled immediately (within 30 min)...
What's NewHigh-volume testing is critical to COVID-19 containment. In this report, we share our team's development of a pediatric-focused drive-through center, and discuss how process can inform future pediatric public health efforts. High-volume Identifying individuals infected by the novel coronavirus mitigating its deleterious effects. Challenges have arisen around test high volumes patients while minimizing risk disease spread. outline approach, process, lessons learned in developing...
Increased intracranial pressure (ICP) caused by edema following severe traumatic brain injury (TBI) or stroke contributes to high rates of mortality and morbidity. The search continues for more effective treatments that target the increased ICP. We previously described effect fixed charge density (FCD) on its swelling behavior according Donnan effect. Here we show reduction tissue FCD is an means reducing in rat porcine cortical vitro. enzymes directed at digesting candidate contributors...
Abstract Diffusely infiltrating gliomas are known to cause alterations in cortical function, vascular disruption and seizures. These neurological complications present major clinical challenges, yet their underlying mechanisms causal relationships disease progression poorly characterized. Here, we followed glioma awake Thy1-GCaMP6f mice using in-vivo wide-field optical mapping monitor both neuronal activity functional hemodynamics. The bilateral synchrony of spontaneous glioma-infiltrated...
Wide-field optical mapping (WFOM) of cortical neural activity and hemodynamics can be achieved in awake, behaving GCaMP animals. Here, we demonstrate the importance correcting for effects hemoglobin absorption this modality.
We explore the use of heat as a contrast mechanism for in-vivo brain imaging, using thermal imaging to understand role dynamic blood flow changes in temperature regulation living brain.
Advancements in brain imaging techniques have significantly expanded the size and complexity of real-time neuroimaging behavioral data. However, identifying patterns, trends synchronies within these datasets presents a significant computational challenge. Here, we demonstrate an approach that can translate time-varying data into unique audiovisualizations consisting audible representations dynamic merged with simplified, color-coded movies spatial components recordings. Multiple variables be...
Bedside ultrasonography, or point-of-care ultrasonography (POCUS), is increasingly becoming widely adopted in pediatric intensive care units (ICU) for procedural interventions and diagnostics. Although vascular access remains one of the primary applications POCUS ICU, its use assessing fluid status responsiveness growing. helps identify reversible causes cardiac arrest, such as pericardial effusion pneumothorax, although impact on outcomes still under investigation. Here, we will review...
Aims & Objectives: Under normal circumstances intracranial pressure (ICP) remains relatively constant during physiologic fluctuations in cardiovascular dynamics and CO2. However, under pathologic conditions like severe traumatic brain injury (sTBI) systemic changes may lead to ICP, reflecting loss of autoregulation and/or poor compliance. We aimed explore the utility ICP responsivity (RICP) determined using high-frequency physiology signals a dynamic, time-varied model ensemble pediatric...