A5101795462- TGF-β signaling in diseases
- Signaling Pathways in Disease
- Galectins and Cancer Biology
- Protease and Inhibitor Mechanisms
- Peptidase Inhibition and Analysis
- Bone Metabolism and Diseases
- MicroRNA in disease regulation
- Hippo pathway signaling and YAP/TAZ
- Cancer-related gene regulation
- Cellular Mechanics and Interactions
- Hedgehog Signaling Pathway Studies
- Wnt/β-catenin signaling in development and cancer
- Bone health and treatments
- Bone and Dental Protein Studies
- Fibroblast Growth Factor Research
- Garlic and Onion Studies
- Angiogenesis and VEGF in Cancer
- Ubiquitin and proteasome pathways
- Liver physiology and pathology
- Fish Biology and Ecology Studies
- Chemotherapy-induced organ toxicity mitigation
- Biomedical Research and Pathophysiology
- Sexual function and dysfunction studies
- PI3K/AKT/mTOR signaling in cancer
- Developmental Biology and Gene Regulation
University of Michigan
2013-2024
Chongqing Medical University
2021
University of South China
2010-2020
Sichuan University
2020
West China Hospital of Sichuan University
2020
National Cancer Institute
2014-2018
Center for Cancer Research
2013-2018
National Institutes of Health
2013-2018
Fujian Medical University
2018
Union Hospital
2018
Abstract Matrix metalloproteinases (MMPs) are endopeptidases that play pivotal roles in promoting tumor disease progression, including angiogenesis. In many solid tumors, MMP expression could be attributed to stromal cells and is partially regulated by tumor-stroma interactions via cell–associated extracellular matrix metalloproteinase inducer (EMMPRIN). The role of EMMPRIN during angiogenesis growth was explored modulating activity using recombinant DNA engineering neutralizing antibodies....
Independent of cathepsin K, the osteoclast-derived matrix metalloproteinases MMP9 and MMP14 codependently drive bone resorption.
Snail and Slug are zinc-finger transcription factors that play key roles in directing the epithelial-mesenchymal transition (EMT) programs associated with normal development as well disease progression. More recent work suggests these EMT-associated also modulate function of both embryonic adult stem cells. Interestingly, YAP TAZ, co-transcriptional effectors Hippo pathway, likewise an important role cell self-renewal lineage commitment. While direct intersections between Snail/Slug pathways...
Cell surface reception of Sonic hedgehog (Shh) must ensure that the graded morphogenic signal is interpreted accordingly in neighboring cells to specify tissue patterns during development. Here, we report endocytic sorting signals for receptor Patched1 (Ptch1), comprising two ‘PPXY’ motifs, direct it degradation lysosomes. These are recognized by HECT-domain ubiquitin E3 ligases, Smurf1 and Smurf2, which induced Shh become enriched Caveolin-1 lipid rafts association with Ptch1....
Cells sense the extracellular environment and mechanical stimuli translate these signals into intracellular responses through mechanotransduction, which alters cell maintenance, proliferation, differentiation. Here we use a mouse model of trauma-induced heterotopic ossification (HO) to examine how cell-extrinsic forces impact mesenchymal progenitor (MPC) fate. After injury, single-cell (sc) RNA sequencing injury site reveals an early increase in MPC genes associated with pathways adhesion...
Here we describe a novel method for imaging apoptosis in cells using near-infrared fluorescent (NIRF) probe selective caspase-1 (interleukin 1beta-converting enzyme, ICE). This biocompatible, optically quenched ICE-NIRF incorporates peptide substrate, which can be selectively cleaved by caspase-1, resulting the release of fluorescence signal. The specificity this is supported various lines evidence: 1) activation purified but not another caspase vitro; 2) infection with herpes simplex virus...
Overexpression of extracellular matrix metalloproteinase inducer (EMMPRIN or CD147), a member the immunoglobulin family and glycoprotein enriched on surface tumor cells, promotes invasion, metastasis, growth survival malignant cells confers resistance to some chemotherapeutic drugs. However, molecular mechanisms underlying actions EMMPRIN are not fully understood. In this study we sought determine whether contributes phenotype breast cancer by inhibiting anoikis, form apoptosis induced loss...
We have previously demonstrated that 40%-70% of elf+/− mice spontaneously develop hepatocellular cancer (HCC) within 15 months, revealing the importance transforming growth factor-beta (TGF-β) signaling pathway in suppressing tumorigenesis liver. The current study was carried out to investigate mechanisms by which embryonic liver fodrin (ELF), a crucial Smad3/4 adaptor, suppresses tumor formation. Histological analysis hyperplastic tissues from revealed abundant newly formed vascular...
Smad ubiquitin regulatory factors (Smurfs) are HECT-domain E3 ligases that regulate diverse cellular processes, including normal and tumor cell migration. However, the underlying mechanism of Smurfs' role in migration is not fully understood. Here we show Smurf1 induces ubiquitination necrosis factor receptor-associated 4 (TRAF4) at K190. Using K190R mutant TRAF4, demonstrate Smurf1-induced required for proper localization TRAF4 to tight junctions confluent epithelial cells. We further...
To improve treatment of obesity, a contributing factor to multiple systemic and metabolic diseases, better understanding state environmental stress at the cellular level is essential. This work presents development three-dimensional (3D) in vitro model adipose tissue displaying induced lipid accumulation as function fatty acid supplementation that, subsequently, investigates responses pro-inflammatory stimulus, thereby recapitulating key stages obesity progression. Three-dimensional spheroid...
Abstract Osteoclasts are bone‐resorbing polykaryons responsible for skeletal remodeling during health and disease. Coincident with their differentiation from myeloid precursors, osteoclasts undergo extensive transcriptional metabolic reprogramming in order to acquire the cellular machinery necessary demineralize bone digest its interwoven extracellular matrix. While attempting identify new regulatory molecules critical resorption, we discovered that murine human osteoclast is accompanied by...
Abstract Transforming growth factor beta (TGF-β) is an important regulator of cell growth, and loss TGF-β signaling a hallmark carcinogenesis. The Smad3/4 adaptor protein β2-spectrin (β2SP) emerging as potent tumorigenesis through its ability to modulate the tumor suppressor function TGF-β. However, date role pathway at specific stages development hepatocellular carcinoma (HCC), particularly in relation activation other oncogenic pathways, remains poorly delineated. Here we identify...
Bone-resorbing osteoclasts mobilize proteolytic enzymes belonging to the matrix metalloproteinase (MMP) family directly degrade type I collagen, dominant extracellular component of skeletal tissues. While searching for additional MMP substrates critical bone resorption, Mmp9/Mmp14 double-knockout (DKO) osteoclasts-as well as MMP-inhibited human osteoclasts-unexpectedly display major changes in transcriptional programs tandem with compromised RhoA activation, sealing zone formation and...
Abstract Aims R ac1, P ak1 and ock1 are indicators related to gastric cancer invasion metastasis, but few reports discuss all three kinds of protein in research on metastasis. The aim this study was investigate the expression clinical significance carcinoma. Methods 158 cases carcinoma were investigated via immunohistochemical staining analysis. Results positive rates normal tissue, intraepithelial neoplastic tissues showed an increasing trend ( < 0.05). Their lymph node metastasis...
This study assessed first the impact of endometrial Tiam1/Rac1 signals and microRNA-22 (miR-22) on embryo implantation in mice, then expression above three genes endometrium during window natural menstrual cycle women with repeated failure (RIF) after vitro fertilization treatment. Four hundred fifty-two Kun-ming female mice 200 (70 infertility patients RIF, 130 as controls) were entered into this study. Endometrial miR-22 studied clinical mouse samples serum estrogen (E2) progesterone (P)...
Abstract Introduction Despite the increased understanding of treadmill training on angiogenesis stroke patients, its mechanism is not clearly known. The metalloproteinase membrane type 1‐metalloprotease ( MT 1‐ MMP ) promotes regeneration peripheral vessels but seldom research cerebral blood vessels. This study was designed to investigate effects exercise and expression after ischemia in rats. Methods adult male Sprague Dawley( SD rats were randomly divided into three groups: sham operation...
Purpose: To study the cellular distribution of extracellular matrix metalloproteinase inducer (EMMPRIN; CD147) in human eye and corneal retinal pigment epithelium cell lines its possible existence as a soluble protein ocular fluids. Methods: Immunohistochemistry was performed on eyes for cultures. Different EMMPRIN isoforms were analyzed by Western blotting Results: immunostaining could be detected conjunctival epithelium, endothelium, stromal keratocytes, several layers nerve fibers optic...
The role of sonic hedgehog (SHH) in maintaining corpora cavernosal morphology the adult penis has been established; however, mechanism how SHH itself is regulated remains unclear. Since decreased protein a cause smooth muscle apoptosis and erectile dysfunction (ED) penis, treatment can suppress cavernous nerve (CN) injury-induced apoptosis, question signaling significant. It likely that neural input involved this process since two models neuropathy-induced ED exhibit increased penis. We...
Nonalcoholic fatty liver disease (NAFLD) is characterized by abnormal accumulation of triglycerides (TG) in the and other metabolic syndrome symptoms, but its molecular genetic causes are not completely understood. Here, we show that mice deficient for ubiquitin ligase (E3) Smad regulatory factor 1 (Smurf1) spontaneously develop hepatic steatosis as they age exhibit exacerbated phenotype under a high-fat diet (HFD). Our data indicate loss Smurf1 up-regulates expression peroxisome...
The microRNA miR-130a-3p (miR-130a-3p) has anti-tumor activity against numerous cancer types. Further, may target Wnt signaling, which is a critical pathway regulating tumorigenesis. Functions of in colorectal (CRC) and contributions Wnt1 modulation, however, have not been examined, hence the exploration on these two aspects. In this study, comparison with normal controls, both CRC tissue multiple cell lines showed downregulated miR-130a-3p. MiR-130a-3p overexpression contributed to decrease...