A5089490204- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cardiovascular Effects of Exercise
- Nitric Oxide and Endothelin Effects
- ECG Monitoring and Analysis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Receptor Mechanisms and Signaling
- Bee Products Chemical Analysis
- Trypanosoma species research and implications
- Cardiac Arrhythmias and Treatments
- Medical and Biological Ozone Research
- Advanced Glycation End Products research
- Insect symbiosis and bacterial influences
- Vector-borne infectious diseases
- Research on Leishmaniasis Studies
- Venomous Animal Envenomation and Studies
- Microencapsulation and Drying Processes
- Parasitic Diseases Research and Treatment
- Pluripotent Stem Cells Research
- Adipose Tissue and Metabolism
- Cardiac Fibrosis and Remodeling
- 3D Printing in Biomedical Research
- Mitochondrial Function and Pathology
- Neuroscience and Neural Engineering
- Healthcare and Venom Research
Universidad de Costa Rica
2024-2025
University of Wisconsin–Madison
2011-2024
University of Michigan
2012-2024
Mayo Clinic
2012
Mayo Clinic in Arizona
2012
Madison Group (United States)
2011
Universidad Nacional
2008-2009
The purpose of this study was to define the relationship in polymicrobial sepsis (in adult male C57BL/6 mice) between heart dysfunction and appearance plasma extracellular histones. Procedures included induction by cecal ligation puncture measurement function using echocardiogram/Doppler parameters. We assessed ability histones cause disequilibrium redox status intracellular [Ca2+]i levels cardiomyocytes (CMs) (from mice rats). also studied disturb both functional electrical responses hearts...
Rationale: Most cardiac ryanodine receptor (RyR2) mutations associated with catecholaminergic polymorphic ventricular tachycardia (CPVT) are postulated to cause a distinctive form of Ca 2+ release dysfunction. Considering the spread distribution CPVT mutations, we hypothesized that dysfunctional heterogeneity also was feasible. Objective: To determine molecular and cellular mechanisms by which novel RyR2-V2475F mutation in humans triggers -dependent arrhythmias whole hearts intact mice....
Abstract Excitation-contraction coupling involves the faithful conversion of electrical stimuli to mechanical shortening in striated muscle cells, enabled by ubiquitous second messenger, calcium. Crucial this process are ryanodine receptors (RyRs), sentinels massive intracellular calcium stores contained within sarcoplasmic reticulum. In response sarcolemmal depolarization, RyRs release into cytosol, facilitating mobilization myofilaments and enabling cell contraction. order for cells relax,...
Background: Natural products, such as propolis, are an important source of biologically active compounds with the potential to treat health disorders. Propolis is a well-known waxy resin recognized for its antimicrobial, immunomodulatory, and cytotoxic effects. Objective: In this study, we aimed clarify formation mechanism propolis nanoparticles from perspective their stability chemical composition. By evaluating light absorption behaviour formed in different media quantifying polyphenols,...
Abstract Chagas disease, caused by the protozoan Trypanosoma cruzi, is a zoonosis primarily found in rural areas of Latin America. It considered neglected tropical and Triatoma dimidiata main vector parasite Central Despite efforts, disease continues to be public health concern, control remains primary tool reduce transmission. In this study, we tested hypothesis that highly abundant bacteria gut T. inhibit growth cruzi. To achieve this, bacterial diversity specimens from Costa Rica was...
Propolis natural extracts have been used since ancient times due to their antioxidant, anti-inflammatory, antiviral, and antimicrobial activities. In this study, we produced scaffolds of type I collagen, extracted from Wistar Hanover rat tail tendons, impregnated them with propolis nanoparticles (NPs) for applications in regenerative medicine. Our results show that the impregnation NPs collagen affected denaturation temperature tensile strength. The changes structural self-assembly contact...
In polymicrobial sepsis, the extracellular histones, mainly released from activated neutrophils, significantly contribute to cardiac dysfunction (septic cardiomyopathy), as demonstrated in our previous studies using Echo-Doppler measurements. This study aims elucidate roles of histones and their interactions with Toll-like receptors (TLRs) dysfunction. Through ex vivo assessments ECG, left ventricle (LV) function parameters, mice perfused we aim provide comprehensive insights into mechanisms...
Abstract During exercise or stress, the sympathetic system stimulates cardiac contractility via β‐adrenergic receptor (β‐AR) activation, resulting in phosphorylation of ryanodine (RyR2). Three RyR2 sites have taken prominence excitation–contraction coupling: S2808 and S2030 are described as protein kinase A specific S2814 a Ca 2+ /calmodulin type‐2‐specific site. To examine contribution these phosphosites to signalling, we generated double knock‐in (DKI) mice which Ser2808 Ser2814 both been...
The in vitro activity of four 2-nitropropene derivatives, 1-(3-benzothienyl)-2-nitropropene (N1), 1-(3-thienyl)-2-nitropropene (N2), 1-(5-bromo-2-thienyl)-2-nitropropene (N3) and 1-(4-bromo-2-thienyl)-2-nitropropene (N4), were tested against cultures the parasite Trypanosoma cruzi. Cytotoxicity studies performed using Vero cells. blood trypomastigotes, amastigotes epimastigotes showed differential degrees sensitivity towards compounds; highest tripomastigotes was exhibited by N1, followed...
<title>Abstract</title> Accurate and timely arrhythmia multi-classification is crucial for diagnosing managing cardiovascular diseases. Traditional interpretation of electrocardiograms time-consuming prone to human error. To address these challenges, this study describes a novel deep learning-based approach automated classification. Our proposed model, convolutional-recurrent neural network, leverages the strengths both convolutional recurrent networks effectively capture spatial temporal...
Despite intensive research, the functional consequences of cardiac ryanodine receptor (RyR2) phosphorylation remain highly controversial. Two major sites, S2808 and S2814, are respective targets for PKA CaMKII form a RyR2 “phosphorylation hot spot”. We previously showed that genetic ablation has no structural or function. Here we generated novel double knock-in mouse with S2814 (S2808A/S2814A) tested hypothesis preventing “hot spot” discernible impact in Echocardiography intraventricular...
Catecholaminergic polymorphic ventricular tachycardia (CPVT) patients harboring the cardiac ryanodine receptor (RyR2) mutation RyR2-A4860G exhibit sympathetically-driven idiopathic fibrillation without structural defects. In sharp contrast to other CPVT mutations characterized date, fails activate in response increasing luminal [Ca 2+ ] and, when expressed HEK293 or HL-1 cells, it exhibits attenuated store overload-induced Ca release. Thus, is first loss-of-function linked CPVT, but...