A5042230052- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Tissue Engineering and Regenerative Medicine
- 3D Printing in Biomedical Research
- Congenital heart defects research
- RNA Interference and Gene Delivery
- Photoreceptor and optogenetics research
- Immune cells in cancer
- Immune Cell Function and Interaction
- Renal and related cancers
- Planarian Biology and Electrostimulation
- Neuroscience and Neural Engineering
- Zebrafish Biomedical Research Applications
- Single-cell and spatial transcriptomics
- Pancreatic function and diabetes
- RNA regulation and disease
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- Nanoplatforms for cancer theranostics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Micro and Nano Robotics
- Mesenchymal stem cell research
- Light effects on plants
- Virus-based gene therapy research
Purdue University West Lafayette
2019-2025
Purdue University Institute for Cancer Research
2023-2024
Davidson College
2024
Center for Cancer Research
2021-2023
University of California, Berkeley
2018-2023
University of Wisconsin–Madison
2012-2019
Institute of Food Science and Technology
2018-2019
Ministry of Agriculture and Rural Affairs
2019
Institute of Agro-Products Processing Science and Technology
2018
Morgridge Institute for Research
2016
Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors may provide the means for vascularization of tissue-engineered constructs can serve as models to study vascular development disease. Here, we report a method efficiently produce from hPSCs via GSK3 inhibition culture in defined media direct hPSC differentiation CD34(+)CD31(+) progenitors. Exogenous growth factor (VEGF) treatment was dispensable, progenitor β-catenin dependent. Furthermore, by clonal analysis,...
We report a defined method for differentiating human pluripotent stem cells to brain endothelial cells.
Abstract Glioblastoma (GBM) is one of the most aggressive and lethal solid tumors in human. While efficacious therapeutics, such as emerging chimeric antigen receptor (CAR)-T cells chemotherapeutics, have been developed to treat various cancers, their effectiveness GBM treatment has hindered largely by blood-brain barrier blood-brain-tumor barriers. Human neutrophils effectively cross physiological barriers display effector immunity against pathogens but short lifespan resistance genome...
Neutrophils, the most abundant white blood cells in circulation, are closely related to cancer development and progression. Healthy primary neutrophils present potent cytotoxicity against various cell lines through direct contact via generation of reactive oxygen species. However, due their short half-life resistance genetic modification, have not yet been engineered with chimeric antigen receptors (CARs) enhance antitumor for targeted immunotherapy. Here, we genetically human pluripotent...
ABSTRACT In embryonic stem cell (ESC) models for early development, spatially and temporally varying patterns of signaling types emerge spontaneously. However, mechanistic insight into this dynamic self-organization is limited by a lack methods spatiotemporal control signaling, the relevance signal dynamics cell-to-cell variability to pattern emergence remains unknown. Here, we combine optogenetic stimulation, imaging transcriptomic approaches study human ESCs (hESC) in two-dimensional (2D)...
Abstract Human pluripotent stem cells (hPSCs) offer tremendous promise in tissue engineering and cell-based therapies due to their unique combination of two properties: pluripotency unlimited proliferative capacity. However, directed differentiation hPSCs clinically relevant cell lineages is needed achieve the goal hPSC-based therapies. This requires a deep understanding how signaling pathways converge on nucleus control ability dissect gene function temporal manner. Here, we report use...
Human pluripotent stem cell (hPSC)-derived endothelial cells and their progenitors are important for vascular research therapeutic revascularization. Here, we report a completely defined progenitor differentiation platform that uses minimalistic medium consisting of Dulbecco's modified eagle ascorbic acid, lacking albumin growth factors. Following hPSC treatment with GSK-3β inhibitor culture in this medium, protocol generates more than 30% multipotent CD34+ CD31+ can be purified to >95% via...
Rapid, robust virus-detection techniques with ultrahigh sensitivity and selectivity are required for the outbreak of pandemic coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2). Here, we report that femtomolar concentrations single-stranded ribonucleic acid (ssRNA) SARS-CoV-2 trigger ordering transitions in liquid crystal (LC) films decorated cationic surfactant complementary 15-mer deoxyribonucleic (ssDNA) probe. More importantly, LC...
Spatially and temporally varying patterns of morphogen signals during development drive cell fate specification at the proper location time. However, current in vitro methods typically do not allow for precise, dynamic spatiotemporal control signaling are thus insufficient to readily study how dynamics affect behavior. Here, we show that optogenetic Wnt/β-catenin pathway activation can be controlled user-defined intensities, temporal sequences, spatial using engineered illumination devices...
Biomimetic artificial surfaces that enable the manipulation of gas bubble mobility have been explored in a wide range applications nanomaterial synthesis, surface defouling, biomedical diagnostics, and therapeutics. Although many superhydrophobic isotropic-lubricant-infused porous developed to manipulate bubbles, simultaneous control over adhesion transport bubbles underwater remains challenge. Thermotropic liquid crystals (LCs), class structured fluids, provide an opportunity tune behavior...
Adoptive chimeric antigen receptor (CAR)-engineered natural killer (NK) cells have shown promise in treating various cancers. However, limited immunological memory and access to sufficient numbers of allogenic donor hindered their broader preclinical clinical applications. Here, we first assess eight different CAR constructs that use an anti-PD-L1 nanobody and/or universal anti-fluorescein (FITC) single-chain variable fragment (scFv) enhance antigen-specific proliferation anti-tumor...
The objective of this experiment was to investigate the effects Lactobacillus plantarum and molasses on nutrient composition, fermentation quality, bacterial count, aerobic stability, microflora alfalfa silage in sandy grasslands. experimental treatments included control (CK), 10 6 CFU/g (L), 5% (M), + (LM). were determined after 14 days 56 ensiling, respectively. results showed that addition L, M, LM reduced dry matter loss (DM), neutral detergent fiber (NDF), acid (ADF) content, increased...
Abstract Extracellular vesicles (EVs) secreted by human brain cells have great potential as cell‐free therapies in various diseases, including stroke. However, because of the significant amount EVs needed preclinical and clinical trials, EV application is still challenging. Vertical‐Wheel Bioreactors (VWBRs) designed features that allow for scaling up generation forebrain spheroid under low shear stress. In this study, secretion spheroids derived from induced pluripotent stem 3D aggregates...
The secreted Wnt signaling molecules are essential to the coordination of cell-fate decision making in multicellular organisms. In adult animals, proteins critical for tissue regeneration and frequently contribute cancer. Small that disable acyltransferase Porcupine (Porcn) candidate anticancer agents clinical testing. Here we have systematically assessed effects Porcn inhibitor (WNT-974) on several types identify potentially unwanted chemical could limit therapeutic utility such agents. An...
Droplet-based miniature reactors have attracted interest in both fundamental studies, for the unique reaction kinetics they enable, and applications bio-diagnosis material synthesis. However, precise automatic feeding of chemicals, important delicate reactions these miniaturized chemical reactors, either requires complex, high-cost microfluidic devices or lacks capability to maintain a pinning-free droplet movement. Here, design synthesis new class liquid crystal (LC)-based open surfaces,...