A5101619661- Pluripotent Stem Cells Research
- Spine and Intervertebral Disc Pathology
- Orthopaedic implants and arthroplasty
- MicroRNA in disease regulation
- Natural Compounds in Disease Treatment
- Circular RNAs in diseases
- Cervical and Thoracic Myelopathy
- Musculoskeletal pain and rehabilitation
- Orthopedic Infections and Treatments
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Inflammasome and immune disorders
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- Infectious Diseases and Tuberculosis
- Cancer-related gene regulation
- Gout, Hyperuricemia, Uric Acid
- Diet, Metabolism, and Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Extracellular vesicles in disease
- Bone health and treatments
- Cancer Research and Treatments
- CAR-T cell therapy research
- Botulinum Toxin and Related Neurological Disorders
- Medical Imaging and Analysis
Soochow University
2020-2025
Hangzhou First People's Hospital
2016-2024
Zhejiang University
2018-2024
Guangdong University of Technology
2023-2024
Harbin Veterinary Research Institute
2024
Chinese Academy of Agricultural Sciences
2024
Macau University of Science and Technology
2021-2023
Hunan University
2023
Indiana University School of Medicine
2020-2022
Indiana University – Purdue University Indianapolis
2020-2022
Neutrophils, the most abundant white blood cells in circulation, are closely related to cancer development and progression. Healthy primary neutrophils present potent cytotoxicity against various cell lines through direct contact via generation of reactive oxygen species. However, due their short half-life resistance genetic modification, have not yet been engineered with chimeric antigen receptors (CARs) enhance antitumor for targeted immunotherapy. Here, we genetically human pluripotent...
Abstract Embryonic stem cells (ESCs) exhibit unique attributes of boundless self-renewal and pluripotency, making them invaluable for fundamental investigations clinical endeavors. Previous examinations microgravity effects on ESC differentiation have predominantly maintained a descriptive nature, constrained by limited experimental opportunities techniques. In this investigation, we present compelling evidence derived from murine human ESCs, demonstrating that simulated (SMG)-induced stress...
Osteoarthritis (OA), in which macrophage-driven synovitis is considered closely related to cartilage destruction and could occur at any stage, an inflammatory arthritis. However, there are no effective targets cure the progression of OA. The NOD-, LRR-,and pyrin domain-containing protein 3 (NLRP3) inflammasome synovial macrophages participates pathological process treatment strategies targeting it be approach for PIM-1 kinase, as a downstream effector many cytokine signaling pathways, plays...
Abstract Osteosarcoma is a malignant bone tumor that primarily inflicts the youth. It often metastasizes to lungs after chemotherapy failure, which eventually shortens patients’ lives. Thus, there dire clinical need develop novel therapy tackle osteosarcoma metastasis. Methionine dependence special metabolic characteristic of most cells may offer target pathway for such therapy. Herein, we demonstrated methionine deficiency restricted growth and metastasis cultured human cells. A genetically...
Abstract Damaged bone is sensitive to mechanical stimulation throughout the remodeling phase of healing. Muscle damage and muscular atrophy associated with open fractures subsequent fixation are not beneficial maintaining optimum conditions for stability. The aim this study was investigate whether local muscle dysfunction affect fracture healing in a rat femur model. We combined model short period quadriceps fracture. Forty‐four‐month‐old male Wistar rats were adopted study. Two units...
We aimed to study the anti-tumor effects of triptolide on osteosarcoma and related molecular mechanisms. The cell viability, apoptosis portion, tumor size, weight, invasion cells were determined. relative level microRNA-181 in tissues adjacent was determined by quantitative real-time reverse transcription polymerase chain reaction. target gene microRNA-181a verified luciferase report assay. At last, treated with + mimics verify relationship between microRNA-181a. Triptolide inhibited...
Background/Aims: Osteosarcoma, the most common primary bone malignancy, arises from primitive transformed cells of mesenchymal origin with worldwide increasing morbidity and mortality. Previous studies found apoptosis osteosarcoma was essential for an effective manner to improve progress osteosarcoma, CXCR4 has been demonstrated be relevant various tumor metastasis. Methods: The proliferation transfected shRNA control were measured by BrdU assay. Apoptosis detected flow cytometry. Apoptotic...
Abstract Background Minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF) and endoscopic (Endo-LIF) are both minimally procedures for degenerative diseases. In this study, we attempted to compare the clinical efficacy postoperative outcomes of MIS-TLIF Endo-LIF Methods The study cohort comprised 99 patients with diseases treated by or from January 2019 July 2021. (visual analogue scale (VAS), Oswestry disability index (ODI), MacNab criteria) preoperatively, 1 month...
The mechanism by which DNA-damage affects self-renewal and pluripotency remains unclear. DNA damage repair mechanisms have been largely elucidated in mutated cancer cells or simple eukaryotes, making valid interpretations on early development difficult. Here we show the impact of ionizing irradiation maintenance differentiation mouse embryonic stem (ESCs). Our findings demonstrate that induces upregulation p53 family genes, including p53, p63, p73, resulting elevated expression E3 ubiquitin...
Porcine epidemic diarrhea virus (PEDV) is associated with severe enteritis, which contributes to high mortality in piglets. The aim of this study was describe molecular mechanisms proinflammatory cytokine(s) production during PEDV infection. We showed that infection porcine intestine epithelial cell clone J2 (IPEC-J2) induces a gradual increase interleukin 8 (IL-8) at different time points, as well Vero E6 PEDV. secretion IL-8 these two lines infected related the activation NF-κB....
Hydroxyapatite (HAP), a CaP compound similar to the mineral phase present in bone, has excellent biocompatibility but little osseous inductivity. In this study, we evaluated novel nano-Sr-HAP, which calcium of hydroxyapatite was substituted with strontium, acts as bone-forming agent. Its and osteoinduction were assayed using marrow mesenchymal stem cells (MSCs) osteoblasts (OBs) vitro. We able demonstrate that nano-Sr-HAP supported increased OB cell adhesion, proliferation viability up 4...
The treatment of lumbar infectious spondylitis is controversial. In this study, we attempted to demonstrate that unilateral percutaneous endoscopic debridement with physiologic saline and negative pressure drainage postoperatively may achieve a satisfactory result in spondylitis. We retrospectively analyzed 17 patients who underwent (PEDD) through posterolateral transforaminal approach. Each biopsy specimen was submitted without delay after surgery examined for microorganisms evaluated...
Rats with fluorescent markers are of great value for studies that trace lineage-specific development, particularly those assessing the differentiation potential embryonic stem cells (ESCs). The piggyBac (PB) transposon is widely used efficient introduction genetic modifications into genomes, and has already been successfully to produce transgenic mice rats. Here, we generated rats carrying either desRed protein (RFP) gene or enhanced green (eGFP) by injecting pronuclei PB plasmids. We showed...
Type 2 diabetes mellitus (T2DM) is a well-known independent risk factor for non-alcoholic fatty liver disease (NAFLD). However, research exploring the association between blood glucose management and of NAFLD status in subjects without was insufficient. This study aimed to explore glycated hemoglobin (HbA1c) with severity steatosis fibrosis non-diabetic people.A cross-sectional analysis conducted on 2998 American adults using data from National Health Nutrition Examination Survey (NHANES)...
The present study was designed to investigate the function of matrix metalloproteinase‑9 (MMP‑9) in human glioma cells and potential regulatory mechanisms. Reverse transcription‑quantitative polymerase chain reaction used analyze expression MMP‑9 microRNA‑34a (miR‑34a) plasma patients with healthy volunteers. MTT Transwell assays were assess cell growth migration, respectively. Annexin‑V/propidium iodide staining measure apoptosis. In addition, measured using western blot analysis. glioma,...