A5100333558- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- Extracellular vesicles in disease
- Muscle Physiology and Disorders
- Cancer Mechanisms and Therapy
- Ferroptosis and cancer prognosis
- Machine Learning in Bioinformatics
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- RNA Interference and Gene Delivery
- Bone Metabolism and Diseases
- Sirtuins and Resveratrol in Medicine
- Cancer, Lipids, and Metabolism
- Adipose Tissue and Metabolism
- Spectroscopy and Chemometric Analyses
- Genomics, phytochemicals, and oxidative stress
- Histone Deacetylase Inhibitors Research
- Silk-based biomaterials and applications
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Chromatin Remodeling and Cancer
- Biomarkers in Disease Mechanisms
- Child Nutrition and Water Access
Longhua Hospital Shanghai University of Traditional Chinese Medicine
2022-2025
Shanghai University of Traditional Chinese Medicine
2022-2025
Shanghai Institute of Technology
2025
XinHua Hospital
2024
Sichuan Agricultural University
2016-2024
Ministry of Agriculture and Rural Affairs
2022-2024
Sun Yat-sen University
2015-2024
Tianjin Medical University General Hospital
2017-2024
Shenzhen Second People's Hospital
2015-2024
Guilin Medical University
2023
Several miRNAs have been implicated in the development and progression of osteosarcoma (OS). In this study, we found that miR‐16 is downregulated OS cell lines tissues. Overexpression suppresses proliferation tumor growth nude mice. Furthermore, confirmed IGF1R a direct target miR‐16. Mechanistic investigation revealed overexpression inhibits Raf1–MEK1/2–ERK1/2 pathway. clinical specimens, levels inversely correlate with expression. Our results provide significant clues regarding role as...
Ascl2, a basic helix-loop-helix transcription factor, is downstream target of WNT signaling that controls the fate intestinal cryptic stem cells and colon cancer progenitor cells. However, its involvement in molecular events largely undefined; particular, mechanism by which Ascl2 regulates plasticity epithelial-mesenchymal transition (EMT) mesenchymal-epithelial (MET) programs remains unknown. In this study, we systematically demonstrate loss function promotes MET derepressing expression...
MicroRNAs (miRNAs) are a class of highly conserved, small endogenous single-strand non-coding RNAs. They aberrantly expressed in the circulation and tissue patients with cancer. Therefore, it has been suggested that they may act as key regulators carcinogenesis. The aim present study was to examine expression level miR-195-5p human breast cancer its potential role measured 40 specimens adjacent normal tissues by quantitative polymerase chain reaction (qPCR). Next, explore function...
Osteogenic differentiation of human mesenchymal stem cells (hMSCs) is regulated by multiple transcription factors and signaling molecules. However, the molecular mechanisms underlying this process remain to be fully elucidated. MicroRNAs (miRNAs) act as key regulators in various biological processes mediating mRNA degradation or translational inhibition target genes. In study, we report that miR-346 plays critical roles regulating osteogenic hBMSCs. The expression endogenous was increased...
Abstract Background MicroRNAs are a class of endogenous single strand non-coding RNAs that involved in many important physiological and pathological processes. The purpose this study was to examine the expression levels miR-497 human breast cancer its function MDA-MB-231 cells. Methods Quantitative polymerase chain reaction used measure 40 specimens adjacent normal tissues. MTT assays, colony formation wound healing transwell assays cell cycle were explore potential Dual-luciferase reporter...
Spermatogenesis is temperature-dependent, and the increase in testicular temperature seriously affects mammalian spermatogenesis semen quality. In this study, heat stress model of mice was made with a 43 °C water bath for 25 min, effects on quality spermatogenesis-related regulators were analyzed. On 7th day after stress, testis weight shrank to 68.45% sperm density dropped 33.20%. High-throughput sequencing analysis showed that 98 microRNAs (miRNAs) 369 mRNAs down-regulated, while 77 miRNAs...
Abstract Recent studies have demonstrated functions of miR-145 in vascular smooth muscle cells (VSMCs) phenotypes and diseases. In this study, we aim to determine whether CD40 is involved mediated switch VSMC phenotypes. cultured VSMCs, the effects on TNF-α, TGF-β, Homocysteine (Hcy) induced cell proliferation were evaluated by over-expression or siRNA-mediated knockdown CD40. We also used ultrasound imaging explore effect carotid artery intima-media thickness (CIMT) atherosclerotic cerebral...
tRNA-derived fragments (tRFs), a novel type of non-coding RNA derived from tRNAs, play an important part in governing gene expressions at post-transcriptional level. To date, the regulatory mechanism tRFs fat deposition and adipogenesis is completely unknown. In this study, high diet was employed to induce obese rat model, transcriptome sequencing conducted identify differentially expressed that response obesity. We found out tRFGluTTC, which promoted preadipocyte proliferation by increasing...
Emerging evidence is revealing that microRNAs (miRNAs) play essential roles in mechanosensing for regulating osteogenesis. However, no mechanoresponsive miRNAs have been identified human bone specimens. Methods: Bedridden and aged patients, hindlimb unloaded mice, Random Positioning Machine primary osteoblasts were adopted to simulate mechanical unloading conditions at the human, animal cellular levels, respectively. Treadmill exercise Flexcell cyclic stretching used loading vivo vitro,...
Glioblastoma multiforme (GBM), WHO grade IV astrocytoma, is the most common primary neoplasm of central nervous system (CNS) and has highest malignancy mortality rates. The invasive nature GBM complicates surgical resection restricts chemotherapeutic access, contributing to poor patient prognosis. migration tumor cells closely related cell proliferation. acquisition migratory capability, in addition intracellular factors, proposed be a crucial mechanism during progression invasion. Using...
Genistein is an isoflavone phytoestrogen that has been shown to improve obesity; however, the underlying molecular mechanisms involved therein have not clearly elucidated. In this study, we administered genistein high-fat diet-induced obese mice investigate its effect on hepatic gluconeogenesis. The results showed treatment significantly inhibited body weight gain, hyperglycemia, and adipose lipid deposition in mice. Glucose tolerance test (GTT), insulin (ITT) pyruvate (PTT) gluconeogenesis...
Evidence suggest that overexpression of hypoxia-inducible factor-1α (HIF-1α) is linked to multidrug resistance epilepsy. Here we explored whether aberrant expression HIF-1α regulated by miRNAs. Genome-wide microRNA profiling was performed on temporal cortex resected from mesial lobe epilepsy (mTLE) patients and age-matched controls. miRNAs are putative regulator were predicted via target scan confirmed real-time quantitative polymerase chain reaction (RT-qPCR). Mimics or miRNA morpholino...
Adipogenesis is a complex biological process and the main cause of obesity. Recently, microRNAs (miRNAs), class small endogenous non-coding RNAs, have been proven to play an important role in adipogenesis by post-transcriptional regulation target genes. In this current study, we observed increment miR-152 expression during 3T3-L1 cell audiogenic differentiation. A functional analysis indicated that overexpression inhibited pre-adipocyte proliferation suppressed some cycle-related Moreover,...
l-arginine (l-Arg) has been reported to possess a wide range of functions, including anti-inflammatory, anti-oxidative, and anti-apoptosis. However, the role l-Arg in LPS-induced muscle injury its potential protective mechanism not well elucidated. This study aimed investigate effects on oxidative stress apoptosis differentiated C2C12 myotube cells. Our results demonstrated that cells treated with 0.2 mg/mL LPS significantly decreased cell viability. treatment suppressed induced ROS...
Neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease, and many other types, cause cognitive dysfunctions such as dementia via the progressive loss of structure or function body's neurons. However, etiology these diseases remains unknown, diagnosing less common disorders vascular (VaD) a challenge. In this work, we developed machine-leaning-based technique to distinguish between normal control (NC), AD, VaD, with Lewy bodies, mild impairment at microRNA (miRNA)...
Adult wound scarring result in functional skin deficits. However, the development of effective measures to modulate entire healing encourage function reconstruction is still a clinical challenge, as multiple cells are involved hierarchically. Hydrogel scaffolds with long-lasting local release provide new insights into relevance healing. Herein, multi-targeting hydrogel loaded SHP099 (Gel-SHP) designed during repair. Our results show that Gel-SHP promotes rapid by modulating macrophages...
MicroRNAs have been reported to be aberrantly expressed in patients with pancreatic cancer. The aim of the present meta-analysis is establish overall diagnostic accuracy measurement microRNA for diagnosing cancer.After a systematic review English language studies from Medline, Embase, and Cochrane Library, sensitivity, specificity, other measures diagnosis cancer were pooled using random-effects models. methodological quality each study was assessed by QUADAS (quality assessment accuracy)....