A5100723771- MicroRNA in disease regulation
- Advanced biosensing and bioanalysis techniques
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Nanopore and Nanochannel Transport Studies
- RNA Research and Splicing
- Cancer-related gene regulation
- Circular RNAs in diseases
- Blood Coagulation and Thrombosis Mechanisms
- Iron Metabolism and Disorders
- Molecular Biology Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Proteomics Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Monoclonal and Polyclonal Antibodies Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hemoglobinopathies and Related Disorders
- Mass Spectrometry Techniques and Applications
- Genomics and Rare Diseases
Shenzhen Maternity and Child Healthcare Hospital
2019-2025
York University
2017-2020
Nanjing University of Chinese Medicine
2018
People's Liberation Army No. 150 Hospital
2013
Immunovaccine (Canada)
2009
Second Military Medical University
2008
Eastern Hepatobiliary Surgery Hospital
2008
MicroRNAs (miRNAs) are small non-coding RNAs (typically consisting of 18-25 nucleotides) that negatively control expression target genes at the post-transcriptional level. Owing to biological significance miRNAs, miRTarBase was developed provide comprehensive information on experimentally validated miRNA-target interactions (MTIs). To date, database has accumulated >13,404 MTIs from 11,021 articles manual curations. In this update, a text-mining system incorporated enhance recognition...
Abstract The effect of thrombin on tumor cell cycle activation and spontaneous growth was examined in synchronized serum-starved lines a model prostate cancer development TRAMP mice. BrdUrd incorporation propidium iodide staining LNCaP cells arrested G0 treated with or serum revealed 48- 29-fold increase S phase cells, respectively, at 8 hours. Similar results were obtained glioblastoma line, T98G. Cell kinases inhibitors high levels p27Kip1 low Skp2 cyclins D1 A. Addition thrombin, TFLLRN,...
Objective To describe the characterization of a novel deletion causing α-thalassemia. Methods The proband was 4-year-old boy who presented with abnormal hematological parameters identified during routine blood investigation conducted for cold. Three common α-globin gene deletions, three mutations, and 17 mutations in β-globin were detected using PCR-flow fluorescence hybridization. Next-generation sequencing (NGS) CNVplex technologies employed to identify potential rare pathogenic mutation...
Accurate quantitation of microRNA (miRNA) in tissue samples is required for validation and clinical use miRNA-based disease biomarkers. Since sample processing, such as RNA extraction, introduces undesirable biases, it advantageous to measure miRNA a crude cell lysate. Here, we report on accurate lysate by CE-based hybridization assay termed direct quantitative analysis multiple miRNAs (DQAMmiR). Accuracy precision were determined lysate, extract from the pure buffer. The results showed that...
Using five bioinformatics analysis software, we identified Golgi protein 73 (GP73) as a putative target of microRNA-27b (miR-27b), which is closely related to various biological processes or diseases such bone metabolism disease, adipose cell and muscle development, pulmonary hypertension, cervical cancer, breast cancer. However, the clinical significance miR-27b in hepatocellular carcinoma (HCC) still unclear. The differential expression HCC adjacent normal liver tissues was measured by...
Thousands of putative microRNA (miRNA)-based cancer biomarkers have been reported, but none has validated for approval by the Food and Drug Administration. One reasons this alarming discrepancy is lack a method that sufficiently robust carrying out validation studies, which may require analysis samples from hundreds patients across multiple institutions pooling results together. The capillary electrophoresis (CE)-based hybridization assay proved to be more than reversed transcription...
Direct quantitative analysis of multiple miRNAs (DQAMmiR) is a hybridization-based assay, in which the excess DNA hybridization probes separated from miRNA-probe hybrids, and hybrids are each other gel-free capillary electrophoresis (CE) using two types mobility shifters: single-strand binding protein (SSB) added to CE running buffer peptide drag tags conjugated with probes. Here we introduce second-generation DQAMmiR, utilizes nucleic acid (PNA) rather than requires no SSB buffer. PNA...
Ideal-filter CE (IFCE) is a method for the selection of affinity binders protein targets from oligonucleotide libraries, example, random-sequence libraries and DNA-encoded in single step partitioning. In IFCE, protein-oligonucleotide complexes unbound oligonucleotides move opposite directions, facilitating very high efficiency their For any given target library, directions only limited range EOF mobilities, which, turn, corresponds to pH ionic strength values running buffer. Rational design...
Here we present the result of our work on achieving sub-pM limit quantitation in direct quantitative analysis multiple miRNAs by capillary electophoresis with laser-induced fluorescence detection. The PDF file contains description results figures, references, and supporting information. <i>raw data.ZIP</i> is archive containing (i) Excel files raw data used to build figures presented (ii) <i>red-me.PDF </i>file explaining how read use files. <i>figure all figure...
Here we present the result of our work on achieving sub-pM limit quantitation in direct quantitative analysis multiple miRNAs by capillary electophoresis with laser-induced fluorescence detection. The PDF file contains description results figures, references, and supporting information. raw data.ZIP is archive containing (i) Excel files data used to build figures presented (ii) red-me.PDF explaining how read use files. figure all Origin Adobe Illustrator formats. Abstract: Thousands putative...