A5100710308- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA Research and Splicing
- MicroRNA in disease regulation
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Glioma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Cancer, Lipids, and Metabolism
- Genetic and Environmental Crop Studies
- Natural Compound Pharmacology Studies
- Ferroptosis and cancer prognosis
- S100 Proteins and Annexins
- Natural Compounds in Disease Treatment
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Plant Reproductive Biology
- Advanced biosensing and bioanalysis techniques
- Plant Virus Research Studies
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Microtubule and mitosis dynamics
- Nanoparticle-Based Drug Delivery
- Textile materials and evaluations
- CRISPR and Genetic Engineering
Chinese PLA General Hospital
2024
Qingdao University
2021
Tianjin Medical University General Hospital
2014-2019
Tianjin Municipal Education Commission
2019
Nanjing Medical University
2016-2017
Jiangsu Province Hospital
2016-2017
Tianjin Medical University
2014
Guangzhou Medical University
2012
CRISPR/dCas9 systems can precisely control endogenous gene expression without interrupting host genomic sequence and have provided a novel feasible strategy for the treatment of cancers at transcriptional level. However, development CRISPR/dCas9-based anti-cancer therapeutics remains challenging due to conflicting requirements design delivery system: cationic membrane-binding surface facilitates tumor accumulation cellular uptake system, but hinders circulating stability in vivo. Here,...
Over 20% of cancer 'driver' genes encode chromatin regulators. Long noncoding RNAs (lincRNAs), which are dysregulated in various cancers, play a critical role dynamics and gene regulation by interacting with key epigenetic It has been previously reported that the lincRNA HOTAIR mediates recruitment polycomb repressive complex 2 (PRC2) leading to aberrant transcriptional silencing tumor suppressor glioma breast cancer. Thus, can serve as promising therapeutic target. Herein, we identified...
Gambogic acid (GA) is a natural compound derived from Chinese herbs that has been approved by the Food and Drug Administration for clinical trials in cancer patients; however, its molecular targets have not thoroughly studied. Here, we report GA inhibits tumor proteasome activity, with potency comparable to bortezomib but much less toxicity. First, acts as prodrug only gains proteasome-inhibitory function after being metabolized intracellular CYP2E1. Second, GA-induced inhibition...
Glioblastoma multiforme (GBM), WHO grade IV astrocytoma, is the most common primary neoplasm of central nervous system (CNS) and has highest malignancy mortality rates. The invasive nature GBM complicates surgical resection restricts chemotherapeutic access, contributing to poor patient prognosis. migration tumor cells closely related cell proliferation. acquisition migratory capability, in addition intracellular factors, proposed be a crucial mechanism during progression invasion. Using...
Antisense long noncoding RNAs (lncRNAs) are reported to play a regulating role in carcinogenesis of various human malignancies. However, the function lncRNAs and their underlying mechanism renal cell carcinoma (RCC) is still unknown. The aims this study investigate expression lncRNA BX357664 RCC explore its lines. As result, was downregulated according previous microarray analysis qualitative real-time polymerase chain reaction. After upregulation BX357664, reduced migration, invasion,...
Aims and background Although glioblastomas infiltrate diffusely into adjacent brain, it is difficult to unequivocally identify the solitary invading glioma cell. It necessary develop coculture models study motility of cells, monitor cellular morphology, movement direction, migration area invasion rate. Methods Cerebral slices were cultured on Millicell-CM membrane inserts in a petri dish. The neuronal viability organizational structure brain sections well maintained by experimental...
Recent advances in single-cell RNA sequencing (scRNA-seq) have endowed researchers with the ability to detect and analyze transcriptomes of individual cancer cells.In present study, 16,128 tumor cells from EGFR wild-type EGFRvIII mutant were profiled by scRNA-seq.Analyses scRNA-seq data both U87MG U87MG-EGFRvIII libraries revealed inherent heterogeneity gene expression biological processes.The stably expressing showed enhanced transcriptional activities a relatively homogeneous pattern,...
ObjectiveThe objective of this study is to investigate the aggressive infiltration glioblastoma into adjacent brain tissue, considering its challenging prognosis. Initially classified as an intergenic non-coding RNA, we aim elucidate functional implications LINC01138 in glioblastoma.MethodGlioma grading was performed utilizing H&E staining, which unveiled distinct nuclear morphology high-grade gliomas. The downregulation glioma tissues corroborated through qRT-PCR and gel electrophoresis,...
Objective To investigate the impact of Cortactin knocking down by specific siRNA on invasion and migration human malignant glioma cells.Methods Immunohistochemistry was employed to detect expression in normal brain tissues glioblastoma tissues,which were collected from patients performed epilepsy surgery removal surgery,respectively.Human cell line U251 routinely cultured vitro,and divided into three groups:Cortactin-siRNA group (Cortactin-siRNA being transfected cells mediated...
Objective To investigate the effect of microRNA-451 (miR-451) on migration and its potential molecular mechanism in glioma cell line U251.Method The expression miR-451 tissues brain was identified by qRT-PCR.U251 cells were divided into two groups,miR-451 inhibitor group control group,for vitro experiments.Oligonucleotide transfected human glionblastoma U251.The each examined Wound Healing assay Transwell assay,and protein GTP-Rac1 Rac1 determined Western blot respectively.Results levels...
Objectives This study aimed to explore the effects of silencing isoprenylcysteine carboxyl methyltransfe-rase (Icmt) through small interfering RNA (siRNA) interference on proliferation and apoptosis tongue squamous cell carcinoma (TSCC). Methods Three siRNA were designed constructed for Icmt gene sequence then transfected into TSCC cells CAL-27 SCC-4 silence expression. The tested divided as follows: groups Icmt-siRNA-1, Icmt-siRNA-2, Icmt-siRNA-3, negative control group, blank group....