A5002956243- Extracellular vesicles in disease
- Mesenchymal stem cell research
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- RNA and protein synthesis mechanisms
- Protist diversity and phylogeny
- Polyamine Metabolism and Applications
- Circular RNAs in diseases
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Tissue Engineering and Regenerative Medicine
- Research on Leishmaniasis Studies
- Chemical Reactions and Isotopes
- Animal Genetics and Reproduction
- Amino Acid Enzymes and Metabolism
- Trypanosoma species research and implications
- Enzyme Production and Characterization
- DNA Repair Mechanisms
- Immune cells in cancer
- Electrospun Nanofibers in Biomedical Applications
- Burn Injury Management and Outcomes
- Effects of Radiation Exposure
- Monoclonal and Polyclonal Antibodies Research
- Cannabis and Cannabinoid Research
University of Wisconsin Carbone Cancer Center
2017-2025
University of Wisconsin–Madison
2007-2025
Pan Genome Systems (United States)
2014
Quintessence Biosciences (United States)
2006-2013
Southern Research Institute
2003
Baxter (United States)
1991-1992
Rosalind Franklin University of Medicine and Science
1986-1988
Glasgow Royal Infirmary
1976
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
1976
Abstract Tendon healing follows a complex series of coordinated events, which ultimately produces mechanically inferior tissue more scar-like than native tendon. More regenerative occurs when anti-inflammatory M2 macrophages play dominant role. Mesenchymal stromal/stem cells (MSCs) are able to polarize an immunophenotype via paracrine mechanisms. We previously reported that coculture CD14+ (MQs) with MSCs resulted in unique M2-like macrophage. recently, we generated using only extracellular...
Depending on the stimuli they encounter, B lymphocytes engage in signaling events that lead to immunity or tolerance. Both responses are mediated through antigen interactions with cell receptor (BCR). Antigen valency is thought be an important parameter signaling, but systematic studies lacking. To explore this issue, we synthesized multivalent ligands of defined valencies using ring-opening metathesis polymerization (ROMP). When mice injected antigens generated by ROMP, only those high...
Abstract Background Hematopoietic acute radiation syndrome (H-ARS) occurring after exposure to ionizing damages bone marrow causing cytopenias, increasing susceptibility infections and death. We others have shown that cellular therapies like human mesenchymal stromal cells (MSCs), or monocytes/macrophages educated ex-vivo with extracellular vesicles (EVs) from MSCs were effective in a lethal H-ARS mouse model. However, given the complexity of generating potential risks using allogeneic...
Positron emission tomography (PET) is a powerful tool for investigating the in vivo behavior of drug delivery systems. We aimed to assess biodistribution extracellular vesicles (EVs), nanosized secreted by cells isolated from various human cell sources using PET. EVs were mesenchymal stromal (MSCs) (MSC EVs), macrophages (Mϕ and melanoma line (A375 EVs) centrifugation conjugated with deferoxamine radiolabeling Zr-89. PET radiolabeled evaluated their tissue tropisms. Our study also...
Clostridium difficile causes antibiotic-associated diarrhea and colitis in humans through the actions of toxin A B on colonic mucosa. At present, broad-spectrum antibiotic drugs are used to treat this disease, patients suffer from high relapse rates after termination treatment. This study examined role both toxins pathogenesis ability orally administered avian antibodies against recombinant epitopes C. difficile-associated disease (CDAD). DNA fragments representing entire gene each were...
Mesenchymal stem cells (MSCs) have immunosuppressive and tissue repair properties, but clinical trials using MSCs to prevent or treat graft-versus-host disease (GVHD) shown mixed results. Macrophages (MØs) are important regulators of immunity can promote regeneration remodeling. We previously that educate MØs toward a unique anti-inflammatory immunophenotype (MSC-educated [MEMs]); however, their implications for in vivo models inflammation not been studied yet. now show comparison with MØs,...
Macrophages are crucial drivers of inflammatory corneal neovascularization and thus potential targets for immunomodulatory therapies. We hypothesized that therapeutic use cornea-derived mesenchymal stromal cells (cMSCs) may alter the function macrophages. found cMSCs can modulate phenotype angiogenic In vitro, induce apoptosis macrophages while preferentially promoting a distinct CD14hi CD16hi CD163hi CD206hi immunophenotype has significantly reduced effects based on in vitro angiogenesis...
In the setting of radiation-induced trauma, exposure to high levels radiation can cause an acute syndrome (ARS) causing bone marrow (BM) failure, leading life-threatening infections, anemia, and thrombocytopenia. We have previously shown that human macrophages educated with mesenchymal stem cells (MSCs) by coculture significantly enhance survival mice exposed lethal irradiation. this study, we investigated whether exosomes isolated from MSCs could replace direct generate exosome (EEMs)....
Abstract Recently, our group used exosomes from mesenchymal stromal/stem cells (MSCs) to simulate an M2 macrophage phenotype, that is, exosome-educated macrophages (EEMs). These EEMs, when delivered in vivo, accelerated healing a mouse Achilles tendon injury model. For the current study, we first tested ability of EEMs reproduce beneficial effects different rodent model, rat medial collateral ligament (MCL) We hypothesized treatment with would reduce inflammation and accelerate healing,...
Background Allogeneic bone marrow transplant (alloBMT) is curative for hematologic malignancies through the graft-versus-tumor (GVT) effect but has been ineffective solid tumors like osteosarcoma (OS). OS expresses CD155 which interacts strongly with inhibitory receptors TIGIT and CD96 also binds to activating receptor DNAM-1 on natural killer (NK) cells. never targeted after alloBMT. Combining adoptively transferred allogeneic NK (alloNK) cells blockade alloBMT may enhance a GVT against OS....
Tunicamycin-resistant variants of Leishmania mexicana were found to contain elevated activity N-acetylglucosamine-1-phosphate transferase and amplified DNA (Kink, J. A., Chang, K.-P. (1987) Proc. Natl. Acad. Sci. U. S. A. 84, 1253-1257). Complete digestion their with restriction endonucleases produced discrete ethidium bromide-staining bands after agarose gel electrophoresis. All four BamHI fragments the cloned separately into pBR322 share no substantial sequence homology. complementary each...
Tunicamycin at 10 micrograms/ml inhibits the growth and infectivity of parasitic protozoan Leishmania mexicana amazonensis. Tunicamycin-resistant variants this parasite were produced by gradual acclimatization cells to increasing concentrations drug up 80 a single-step selection ethyl methanesulfonate-pretreated or differentiating leishmanias with micrograms/ml. Prolonged exposure increases stability resistance those resistant contain amplified DNA, which hybridizes in proportion cells'...
A parasitic protozoan, Leishmania mexicana amazonensis, was previously made resistant to tunicamycin (J.A. Kink and K.-P. Chang, Proc. Natl. Acad. Sci. USA 84:1253-1257, 1987). In the present study, six different tunicamycin-resistant variants were biologically biochemically compared with their parental wild type further delineate mechanism of resistance that virulence observed. contrast type, all found grow differentiate in tunicamycin-containing medium. The 50% lethal doses for 10 or 80...
Site-specific cross-linking can generate homogeneous multimeric proteins of defined valency. Pancreatic-type ribonucleases are an especially attractive target, as their natural dimers enter mammalian cells, evade the cytosolic ribonuclease inhibitor (RI), and exert toxic ribonucleolytic activity. Here, we report on use eight distinct thiol-reactive reagents to produce dimeric trimeric conjugates four pancreatic-type ribonucleases. Both site conjugation and, a lesser extent, propinquity...