A5021638897- Corneal Surgery and Treatments
- Ocular Surface and Contact Lens
- Corneal surgery and disorders
- Glaucoma and retinal disorders
- Ocular Infections and Treatments
- Mesenchymal stem cell research
- Ocular Disorders and Treatments
- Intraocular Surgery and Lenses
- Wound Healing and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Electrospun Nanofibers in Biomedical Applications
- Retinal and Optic Conditions
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Academic Publishing and Open Access
- Autoimmune Bullous Skin Diseases
- Optical Coherence Tomography Applications
- Herpesvirus Infections and Treatments
- Clinical Reasoning and Diagnostic Skills
- Connexins and lens biology
- Urticaria and Related Conditions
- Artificial Intelligence in Healthcare and Education
- Academic integrity and plagiarism
- Health and Medical Research Impacts
- scientometrics and bibliometrics research
University of Illinois Chicago
2016-2025
Ocular Surface Center
2022-2025
University of Illinois Urbana-Champaign
2008-2025
New York Eye and Ear Infirmary
2012-2025
University of Southern California
2024
Illinois College
2019-2024
Southern California Eye Institute
2024
Visual Sciences (United States)
2017-2021
University of Chicago
2018-2021
University of California, Irvine
2019
Purpose: Mesenchymal stromal cells (MSCs) have been used therapeutically to modulate inflammation and promote repair. Extracellular vesicles, including exosomes, identified as one of the important mediators. This study investigated effect human corneal MSC-derived exosomes on epithelial wound healing. Methods: Corneal MSCs (cMSCs) were isolated from cadaver corneas. The secretome was collected after 72 hours using differential ultracentrifugation. Morphology size examined by electron...
Purpose This study aimed to report the abilities of large language model ChatGPTR (OpenAI, San Francisco, USA) in constructing ophthalmic discharge summaries and operative notes.Methods A set prompts was constructed through statements incorporating common surgeries across subspecialties cornea, retina, glaucoma, paediatric ophthalmology, neuro-ophthalmology, plastics surgery. The responses ChatGPT were assessed by three surgeons carefully analyzed them for evidence-based content, specificity...
Purpose: The aim of this study was to assess the capabilities ChatGPT-4.0 and ChatGPT-3.5 for diagnosing corneal eye diseases based on case reports compare with human experts. Methods: We randomly selected 20 cases including infections, dystrophies, degenerations from a publicly accessible online database University Iowa. then input text each description into asked provisional diagnosis. finally evaluated responses correct diagnoses, compared them diagnoses made by 3 specialists (human...
The skin is subject to damage from the surrounding environment. repair of wounds can be very challenging due several factors such as severe injuries, concomitant infections, or comorbidities diabetes. Different drugs and wound dressings have been used treat wounds. Tissue engineering, a novel therapeutic approach, revolutionized treatment regeneration tissue damage. This field includes use synthetic natural biomaterials that support growth tissues organs outside body. Accordingly, demand for...
Extracellular vesicles (EVs), a diverse group of cell-derived exocytosed particles, are pivotal in mediating intercellular communication due to their ability selectively transfer biomolecules specific cell types. EVs, composed proteins, nucleic acids, and lipids, taken up by cells affect variety signaling cascades. Research the field has primarily focused on stem with particular focus mesenchymal cells, for potential therapeutic benefits. Recently, tissue-specific EVs or type-specific...
Herpesviruses exemplified by herpes simplex virus-1 (HSV-1) attach to cell surface heparan sulfate (HS) for entry into host cells. However, during a productive infection, the HS moieties on parent cells can trap newly exiting viral progenies and inhibit their release. Here we demonstrate that HS-degrading enzyme of host, heparanase (HPSE), is upregulated through NF-kB translocated upon HSV-1 infection removal facilitate We also find significant increase in HPSE release vivo murine corneas...
In this project, we strived to develop a decellularized human cornea use as scaffold for reconstructing the corneal epithelium and anterior stroma. Human cadaver corneas were by five different methods, including detergent- nondetergent-based approaches. The success of each method on removal cells from was investigated. structural integrity compared with native electron microscopy. basement membrane analyzed histology expression collagen type IV, laminin, fibronectin. Finally, ability support...
Abstract Severe corneal injuries often result in permanent vision loss and remain a clinical challenge. Human bone marrow-derived mesenchymal stem cells (MSCs) their secreted factors (secretome) have been studied for antiscarring, anti-inflammatory, antiangiogeneic properties. We aimed to deliver lyophilized MSC secretome (MSC-S) within viscoelastic gel composed of hyaluronic acid (HA) chondroitin sulfate (CS) as way enhance re-epithelialization reduce complications after mechanical chemical...
Corneal injuries are a major cause of blindness worldwide. To restore corneal integrity and clarity, there is need for regenerative bio-integrating materials in-situ repair replacement tissue. Here, we introduce Light-curable COrnea Matrix (LC-COMatrix), tunable material derived from decellularized porcine cornea extracellular matrix containing un-denatured collagen sulfated glycosaminoglycans. It functionalized hydrogel with proper swelling behavior, biodegradation, viscosity that can be...
Abstract Purpose Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) have been demonstrated to possess great potential in preclinical models. An efficient biomanufacturing platform is necessary for scale up production clinical therapeutic applications. The aim of this study investigate the differences neuro-regenerative properties MSC-derived EVs generated 2D versus 3D culture systems. Method Human bone marrow MSCs (BM-MSCs) were cultured monolayer and bioreactor...
Mesenchymal stromal/stem cells (MSCs) and their secreted factors have been shown to immunomodulatory regenerative effects. In this study, we investigated human bone-marrow-derived MSC secretome (MSC-S) for the treatment of corneal epithelial wounds. Specifically, evaluated role extracellular vesicles (EV)/exosomes in mediating wound-healing effects MSC-S. vitro studies using showed that MSC-CM increased cell proliferation HCEC HCLE cells, while EV-depleted lower both lines compared group....
Inflammatory skin disorders result in significant epidermal changes, including keratinocyte hyperproliferation, incomplete differentiation, and impaired barrier. Here we test whether, conversely, an barrier can promote inflammatory response. Mice lacking the transcription factor Kruppel-like 4 (Klf4) have a severe defect acquisition. Transcription profiling of Klf4–/– newborn revealed similar changes gene expression to involved psoriatic plaques, upregulation gap junction protein connexin 26...