A5039060062- Burkholderia infections and melioidosis
- Chemical Looping and Thermochemical Processes
- SARS-CoV-2 and COVID-19 Research
- Advancements in Semiconductor Devices and Circuit Design
- PARP inhibition in cancer therapy
- Brucella: diagnosis, epidemiology, treatment
- Fungal Infections and Studies
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- CAR-T cell therapy research
- Antifungal resistance and susceptibility
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Electrostatic Discharge in Electronics
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Animal Virus Infections Studies
- Toxoplasma gondii Research Studies
- SARS-CoV-2 detection and testing
- interferon and immune responses
- Viral-associated cancers and disorders
- Receptor Mechanisms and Signaling
- Antimicrobial Peptides and Activities
- Helicobacter pylori-related gastroenterology studies
Northern Arizona University
2016-2025
University of Wisconsin–Madison
2007-2014
Pan Genome Systems (United States)
2014
Institute of Virology of the Slovak Academy of Sciences
2010
During routine screening for
The COVID-19 pandemic demonstrated the critical need for accurate and rapid testing virus detection. This has generated a high number of new methods aimed at replacing RT-PCR, which is golden standard testing. Most techniques are based on biochemistry require chemicals that often expensive supply might become scarce in large crisis. In present paper we suggest use physics leverage novel nanomaterials. We demonstrate using Surface Enhanced Raman Spectroscopy (SERS) virion particles very...
Abstract Engineered natural killer (NK) cells represent a promising option for immune therapy due to their immediate availability in allogeneic settings. Severe acute diseases, such as COVID-19, require targeted and intervention. Here we show engineering of NK express (1) soluble interleukin-15 (sIL15) enhancing survival (2) chimeric antigen receptor (CAR) consisting an extracellular domain ACE2, targeting the spike protein SARS-CoV-2. These CAR (mACE2-CAR_sIL15 cells) bind VSV-SARS-CoV-2...
Burkholderia pseudomallei is a Gram-negative, facultative intracellular bacillus that causes the disease melioidosis. B. expresses number of proteins contribute to its survival in mammalian host. We previously demonstrated immunization with OMVs derived from grown nutrient-rich media protects mice against lethal disease. Here, we evaluated if under macrophage-mimicking growth conditions could be enriched intracellular-stage order improve vaccine. show produced this manner (M9 OMVs) contain...
Positive-strand RNA [(+)RNA] viruses invariably replicate their genomes on modified intracellular membranes. In infected Drosophila cells, Flock House nodavirus (FHV) replication complexes form outer mitochondrial membranes inside ∼50-nm, virus-induced spherular invaginations similar to replication-linked spherules induced by many (+)RNA at various To better understand complex assembly, we studied the mechanisms of FHV spherule formation. has two genomic RNAs; RNA1 encodes multifunctional...
The bacterium Burkholderia ubonensis is commonly co-isolated from environmental specimens harbouring the melioidosis pathogen, pseudomallei. B. has been reported in northern Australia and Thailand but not North America, suggesting similar geographic distribution to Unlike most other cepacia complex (Bcc) species, considered non-pathogenic, although its virulence potential tested. Antibiotic resistance ubonensis, particularly towards drugs used treat severe pseudomallei infections, also...
Abstract Background Valley fever (VF) is a common cause of community acquired pneumonia in the American Southwest. Diagnosis challenging due to VF’s similarities other pulmonary diseases. The VF T-cell response important control disease and measuring this holds promise as an improved diagnostic. Objective Identification epitopes development diagnostics efficacy monitoring for vaccine trials. Methods We identified specific by using their ability bind Major Histocompatibility Complex Class II...
The COVID-19 pandemic has triggered the first widespread vaccination campaign against a coronavirus. Many vaccinated subjects are previously naive to SARS-CoV-2; however, almost all have encountered other coronaviruses (CoVs), and role of this immunity in shaping vaccine response remains uncharacterized. Here, we use longitudinal samples highly multiplexed serology identify mRNA-1273 vaccine-induced antibody responses range CoV Spike epitopes, both phylogenetically conserved non-conserved...
The molecular mechanisms by which RNA viruses induce apoptosis and apoptosis-associated pathology are not fully understood. Here we show that flock house virus (FHV), one of the simplest (family, Nodaviridae), induces robust permissive Drosophila Line-1 (DL-1) cells. To define pathway FHV triggers in this model invertebrate system, investigated potential role apoptotic effectors during infection. Suggesting involvement host caspases, pancaspase inhibitor...
Although acute melioidosis is the most common outcome of Burkholderia pseudomallei infection, we have documented a case, P314, where disease severity lessened with time, and pathogen evolved towards commensal relationship host. In current study, used whole-genome sequencing to monitor this long-term symbiotic better understand B. persistence in P314's sputum despite intensive initial therapeutic regimens. We collected sequenced 118 isolates from airways over >16-year period, also sampled...
New therapeutics are urgently needed in the fight against COVID-19. Repurposing drugs that either already approved for human use or advanced stages of approval process can facilitate more rapid advances toward this goal.
Clostridioides difficile infection (CDI) is an emerging public health threat and C. the most common cause of antimicrobial-associated diarrhea worldwide leading hospital-associated infections in US, yet burden community-acquired (CAI) poorly understood. Characterizing isolated from canines important for understanding role that may play CAI. In addition, several studies have suggested carry toxigenic asymptomatically, which imply there are mechanisms responsible resistance to CDI could be...
Distinct
The antiviral peptide, entry blocker (EB), inhibits influenza virus replication by preventing attachment to cells. Here, we identified the minimal and optimal EB sequence that retained activity with a 50% inhibitory concentration (IC(50)) effective (EC(50)) similar those of full-length peptide several truncated variants possessed up 10-fold lower IC(50)s. These data have implications for improving efficacy EB-derived peptides while decreasing production costs easing synthesis.
ABSTRACT A chronic infection with the parasite Toxoplasma gondii has previously been shown to protect mice against subsequent viral, bacterial, or protozoal infections. Here we have that a T. can prevent Plasmodium berghei ANKA-induced experimental cerebral malaria (ECM) in C57BL/6 mice. Treatment soluble antigens (STAg) reduced sequestration and T cell infiltration brains of P. -infected Administration STAg also preserved blood-brain barrier function, ECM symptoms, significantly decreased...
Abstract Serine proteases (SP), including furin, trypsin, and TMPRSS2 cleave the SARS-CoV-2 spike (S) protein, enabling virus to enter cells. Here, we show that factor (F) Xa, an SP involved in blood coagulation, is upregulated COVID-19 patients. In contrast other SPs, FXa exerts antiviral activity. Mechanistically, cleaves S preventing its binding ACE2, thus blocking viral entry infection. However, less effective against variants carrying D614G mutation common all pandemic variants. The...
Burkholderia pseudomallei causes melioidosis, a common source of pneumonia and sepsis in Southeast Asia Northern Australia that results high mortality rates. A caprine melioidosis model aerosol infection leads to systemic has the potential characterize humoral immune response. This could help identify immunogenic proteins for new diagnostics vaccine candidates. Outbred goats may more accurately mimic human infection, contrast inbred mouse models used date. B. was delivered as an...
Burkholderia pseudomallei is the causative agent of melioidosis, a severe infection endemic to many tropical regions. Lipopolysaccharide (LPS) recognized as an important virulence factor used by B. Isolates have been shown express one four different types LPS (typical LPS, atypical B and B2, rough LPS) in vitro studies demonstrated that may impact disease severity. The association between clinical manifestations, however, still unknown, part because effective method for type identification...
Background Binge drinking, an increasingly common form of alcohol consumption, is associated with increased mortality and morbidity; yet, its effects on the immune system's ability to defend against infectious agents are poorly understood. Burkholderia pseudomallei, causative agent melioidosis can occur in healthy humans, yet binge use progressively being recognized as a major risk factor. Although our previous studies demonstrated that exposure results reduced alveolar macrophage function...
We recently published a preliminary assessment of the activity poly (ADP-ribose) polymerase (PARP) inhibitor, stenoparib, also known as 2X-121, which inhibits viral replication by affecting pathways host. Here we show that stenoparib effectively SARS-CoV-2 wild type (BavPat1/2020) strain and four additional variant strains; alpha (B.1.1.7), beta (B.1.351), delta (B.1.617.2) gamma (P.1) in vitro , with 50% effective concentration (EC 50 ) estimates 4.1 μM, 8.5 24.1 8.2 μM 13.6 respectively. A...
Burkholderia pseudomallei , the causative agent of melioidosis, is a gram-negative soil bacterium well recognized in Southeast Asia and northern Australia. However, wider expanding global distribution B . has been elucidated. Early diagnosis critical for commencing specific therapy required to optimize outcome. Serological testing using indirect hemagglutination (IHA) antibody assay long used augment melioidosis monitor progress. cross reactivity prior exposure may complicate current...
Background Binge drinking, an increasingly common form of alcohol use disorder, is associated with substantial morbidity and mortality; yet, its effects on the immune system's ability to defend against infectious agents are poorly understood. Burkholderia pseudomallei, causative agent melioidosis can occur in healthy humans, yet binge intoxication being recognized as a major risk factor. Although our previous studies demonstrated that exposure increased B. pseudomallei near-neighbor...