A5027155201- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- Cervical Cancer and HPV Research
- Glioma Diagnosis and Treatment
- Endometrial and Cervical Cancer Treatments
- Autoimmune and Inflammatory Disorders Research
- Immune cells in cancer
- NF-κB Signaling Pathways
- Cancer Cells and Metastasis
- Biomarkers in Disease Mechanisms
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Fractional Differential Equations Solutions
- Cellular Mechanics and Interactions
- RNA Research and Splicing
- FOXO transcription factor regulation
- Eosinophilic Disorders and Syndromes
- Animal Virus Infections Studies
- Radiomics and Machine Learning in Medical Imaging
- 3D Printing in Biomedical Research
- Phagocytosis and Immune Regulation
- Virus-based gene therapy research
Soochow University
2023-2025
First Affiliated Hospital of Soochow University
2023-2025
City Of Hope National Medical Center
2021-2024
Changchun Normal University
2023
Fujian Provincial Cancer Hospital
2022
Fujian Medical University
2022
Fujian Women and Children Hospital
2022
Shanxi Normal University
2019-2020
The Ohio State University
2018
Dalian Institute of Chemical Physics
2014
Abstract IL15 is a pleiotropic cytokine with multiple roles that improve immune responses to tumor cells. Oncolytic viruses (OV) specifically lyse tumors and activate responses. Systemic administration of or its complex the IL15Rα chimeric antigen receptor (CAR) natural killer (NK) cells are currently being tested in clinic. Here, we generated herpes simplex 1–based OV-expressing human IL15/IL15Rα sushi domain fusion protein (named OV-IL15C), as well off-the-shelf EGFR-CAR NK cells, studied...
Abstract Engineered natural killer (NK) cells represent a promising option for immune therapy due to their immediate availability in allogeneic settings. Severe acute diseases, such as COVID-19, require targeted and intervention. Here we show engineering of NK express (1) soluble interleukin-15 (sIL15) enhancing survival (2) chimeric antigen receptor (CAR) consisting an extracellular domain ACE2, targeting the spike protein SARS-CoV-2. These CAR (mACE2-CAR_sIL15 cells) bind VSV-SARS-CoV-2...
The majority of patients with acute myeloid leukemia (AML) succumb to the disease or its complications, especially among older patients. Natural killer (NK) cells have been shown antileukemic activity in AML; however, our knowledge, primary NK armed a chimeric antigen receptor (CAR) targeting antigens associated AML as an "off-the-shelf" product for control not explored. We developed frozen, off-the-shelf allogeneic human engineered CAR recognizing FLT3 and secreting soluble interleukin-15...
Glioblastoma (GBM) is the most common brain tumor and remains incurable. Primary GBM cultures are widely used tools for drug screening, but there a lack of genomic pharmacological characterization these primary cultures. Here, we collect 50 patient-derived glioma cell (PDGC) lines characterize them by whole genome sequencing, RNA response screening. We identify three molecular subtypes among PDGCs: mesenchymal (MES), proneural (PN), oxidative phosphorylation (OXPHOS). Drug profiling reveals...
Significance Natural killer (NK) cells belong to a critical innate arm of host immunity against viral infection and malignancies. However, limited expansion persistence NK in vivo remain major challenges for cell-based therapy. Here, we show that platelet-derived growth factor (PDGF)-D−PDGF receptor-beta signaling—a potent stimulator cell motility—activates an autocrine manner contributes interleukin-15–mediated survival but not effector functions, the latter which were previously shown...
We described previously a human natural killer (NK) cell population that upregulates PD-L1 expression upon recognizing and reacting to tumor cells or exposure combination of IL12, IL18, IL15. Here, investigate the safety efficacy tumor-reactive cytokine-activated (TRACK) NK cells, from umbilical cord blood were expanded, transduced with retroviral vector encoding soluble (s) IL15, further cytokine activated induce expression. Our results show cryopreserved thawed sIL15_TRACK had...
Abstract Trogocytosis is a fast, cell–cell contact-dependent uptake of membrane patches and associated molecules by one cell from another. Here, we report our investigation trogocytosis TYRO3, protein, tumor target cells to natural killer (NK) the functional consequences for NK cells. We found that although did not express endogenous TYRO3 on surface, activated rapidly acquired via in vitro vivo. which termed TYRO3+ cells, had significantly enhanced cytotoxicity IFNγ production as well...
The E3 ubiquitin ligase Cbl-b has been characterized as an intracellular checkpoint in T cells; however, the function of primary human NK cells, innate immune anti-tumor effector cell, is not well defined. In this study, we show that expression significantly upregulated cells activated by IL-15, IL-2, and cell-sensitive tumor cell line K562 lacks MHC class I expression. Pretreatment with JAK or AKT inhibitors prior to IL-15 stimulation reversed upregulation. Downregulation resulted...
Abstract Purpose This study aimed to analyse the genomic alteration profiles and immune characteristics of a cohort Chinese cervical cancer patients understand why certain benefited from molecular targeted therapies immunotherapy as well their prognostic significance. Methods PD-L1 expression clinicopathological information were obtained 98 patients. Differences in gene mutations between squamous cell carcinoma (SCC) adenocarcinoma (AC) analysed by chi-square test or Fisher's exact test. our...
Natural products and their derivatives have long been used as pharmacological agents in the fight against cancer. Human natural killer (NK) cells are critical our immune system that they capable of destroying tumor directly. However, there few reports elucidate role activating NK cells. In this study, we discovered a synthetic disaccharide derivative diphyllin, 4-O-{[2",3",4"-tri-O-acetyl-α-D-arabinopyranosyl-(1"→4')]-2',3'-di-O-acetyl-α-L-rhamnopyranosyl}diphyllin (TAARD), can alone...
Pancreatic cancer (PC), the third leading cause of cancer-related death in U.S., is frequently found too late to be cured by traditional chemotherapy. Expression B7 homolog 6 (B7H6), a member family immunoreceptors, has been PC and several other cancers. B7H6 ligand for cytotoxicity triggering receptor 3 (NKp30), which expressed on NK cells. Here, we demonstrate that can detected tissues but not normal organs. Its expression patients associated significantly with tumor differentiation grade...
Abstract Purpose: This study aimed to analyze genomic alteration profiles and immune characteristics of a cohort Chinese cervical cancer patients understand why certain benefited from molecular targeted therapies, immunotherapy their prognostic significance. Methods: PD-L1 expression, clinicopathological information 98 samples. Differences in expression gene mutations between squamous cell carcinoma (SCC) adenocarcinoma (AC) were analyzed by chi-square test or Fisher's exact test. our the...
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