A5056910523- Pulmonary Hypertension Research and Treatments
- Monoclonal and Polyclonal Antibodies Research
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Multiple Sclerosis Research Studies
- T-cell and B-cell Immunology
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Glycosylation and Glycoproteins Research
- Occupational and environmental lung diseases
- SARS-CoV-2 and COVID-19 Research
- Immunotherapy and Immune Responses
- Eosinophilic Disorders and Syndromes
- RNA modifications and cancer
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Alkaline Phosphatase Research Studies
- Sarcoidosis and Beryllium Toxicity Research
- Medical Imaging and Pathology Studies
- Eicosanoids and Hypertension Pharmacology
- Myasthenia Gravis and Thymoma
- Pleural and Pulmonary Diseases
- S100 Proteins and Annexins
- ATP Synthase and ATPases Research
University of Arizona
2011-2025
University of Phoenix
2022-2023
St. Joseph's Hospital and Medical Center
2007-2011
Barrow Neurological Institute
2007-2011
University of California, San Francisco
2005
All India Institute of Medical Sciences
1997-2005
Council of Scientific and Industrial Research
2002
Oxygen is a critical noncellular component of the bone marrow microenvironment that plays an important role in development hematopoietic cell lineages. In this study, we investigated impact low oxygen (hypoxia) on ex vivo myeloerythroid differentiation human cord blood-derived CD34+ stem and progenitor cells. We characterized culture conditions to demonstrate inhibits proliferation causes metabolic shift populations. found hypoxia promotes erythroid by supporting Hypoxia also increases...
Abstract The homeostasis of CD4 + CD25 regulatory T cells (Tregs) depends on the cytokine interleukin (IL)‐2. As IL‐21 shares sequence homology with IL‐2 and receptors contain a γ‐chain common to IL‐2, we hypothesized that could also affect Tregs. We tested this hypothesis in experimental autoimmune encephalomyelitis (EAE), an animal model relapsing‐remitting human multiple sclerosis. show blockade SJL/J mice before after induction EAE enhances influx inflammatory into central nervous system...
Abstract Background Valley fever (VF) is a common cause of community acquired pneumonia in the American Southwest. Diagnosis challenging due to VF’s similarities other pulmonary diseases. The VF T-cell response important control disease and measuring this holds promise as an improved diagnostic. Objective Identification epitopes development diagnostics efficacy monitoring for vaccine trials. Methods We identified specific by using their ability bind Major Histocompatibility Complex Class II...
Abstract Diagnosis of malignant pleural effusion (MPE) is made by cytological examination fluid or histological tissue from biopsy. Unfortunately, detection malignancy using cytology has an overall sensitivity 50%, and dependent upon tumor load, volume assessed, cytopathologist experience. The diagnostic yield also compromised low abundance cells when morphology obscured inflammation reactive mesothelial cells. A reliable molecular marker that may complement for the diagnosis needed. purpose...
Because presentation of acetylcholine receptor (AChR) peptides to T cells is critical the development myasthenia gravis, we examined role cathepsin S (Cat S) in experimental autoimmune gravis (EAMG) induced by AChR immunization. Compared with wild type, Cat null mice were markedly resistant EAMG, and showed reduced B cell responses AChR. immunized immunodominant weak responses, indicating failed peptide accounted for resistance. A inhibitor suppressed vitro IFN-gamma production lymph node...
Rare genetic variants and variation at loci in an enhancer SOX17 (SRY-box transcription factor 17) are identified patients with idiopathic pulmonary arterial hypertension (PAH) PAH congenital heart disease. However, the exact role of or mutations pathogenesis has not been reported.
Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal lung disease with poor prognosis. Current/available clinical prediction tools have limited sensitivity accuracy when evaluating outcomes of IPF. Research has shown that focal adhesion kinase (FAK), produced by the protein tyrosine 2 (PTK2) gene, crucial in IPF development. FAK activation characteristic lesional fibroblasts; Thus, may be valuable therapeutic target or prognostic biomarker for This study aimed to create gene...
Pulmonary arterial hypertension (PAH) is a devastating disease characterized by obliterative vascular remodeling and persistent increase of resistance, leading to right heart failure premature death. Understanding the cellular molecular mechanisms will help develop novel therapeutic approaches for PAH patients. Single-cell RNA sequencing (scRNAseq) analysis found that both FABP4 FABP5 were highly induced in endothelial cells (ECs) Egln1Tie2Cre (CKO) mice, which was also observed pulmonary...
Glatiramer acetate (GA) is an immunomodulatory drug approved for the treatment of clinically isolated syndrome (CIS) and relapsing/remitting multiple sclerosis (RRMS). As antigen-based therapy, GA induces GA-specific antibodies in treated patients animals. do not neutralize therapeutic effects on relapses disability. Rather, it has been suggested that may be associated with improved clinical outcomes. We evaluated antibody responses eight RRMS 15 months GA-treated C57BL/6 mice before after...
Abstract Background The isozymes of alkaline phosphatase, the tissue non-specific, intestinal and placental, have similar properties a high degree identity. placental isozyme (PLAP) is an oncofetal antigen expressed in several malignancies including choriocarcinoma, seminoma ovarian carcinoma. We had earlier attempted to isolate PLAP-specific scFv from synthetic human immunoglobulin library but were unable do so, presumably because similarity between isozymes. In this work, we employed...
Summary By regulating invariant (Ii) chain processing and MHC class II peptide loading, the endosomal protease cathepsin S (Cat S) has a potential role in autoimmune susceptibility. Indeed, Cat null mice are resistant to I‐Ab ‐ restricted experimental myasthenia gravis due inadequate presentation. To explore of Graves' disease model, I‐Ad‐restricted wild‐type (WT) –/– were immunized with adenovirus encoding A subunit thyroid stimulating hormone receptor (TSHR). TSHR develop Th1 T cells,...
Abstract The development of committed erythroid progenitors and their continued maturation into mature erythrocytes requires the cytokine erythropoietin (Epo). Here, we describe immunophenotypic identification a unique Epo-dependent colony-forming unit-erythroid (CFU-E) cell subtype that forms during early erythropoiesis (EE). This previously undescribed CFU-E subtype, termed late-CFU-E (lateC), lacks surface expression characteristic marker CD235a (glycophorin A) but has high levels CD71...
Human antibodies against specific targets of tumor cells are the most desirable molecules for possible immunotherapy. They could be developed by using combinatorial antibody library displayed on a phage. We selected four human fragments (scFv) binding to oncoplacental antigen Heat Stable Alkaline Phosphatase (HSAP, placental isozyme alkaline phosphatase) from synthetic library. Characterization these scFvs showed they bound HSAP with moderate affinity but did not have specificity, as...
Rare genetic variants and variation at loci in an enhancer SRY-Box Transcription Factor 17 (SOX17) are identified patients with idiopathic pulmonary arterial hypertension (PAH) PAH congenital heart disease. However, the exact role of or mutation SOX17 pathogenesis has not been reported.To investigate deficiency (PH) development.Human lung tissue endothelial cells (ECs) from IPAH were used to determine expression SOX17. Tie2Cre-mediated EC-specific deletion Sox17 mice assessed for PH...
Abstract Background Diagnosis of malignant pleural effusion (MPE) is made by cytological examination fluid or histological tissue from biopsy. Unfortunately, detection malignancy using cytology has an overall sensitivity 50%, and dependent upon tumor load, volume assessed, cytopathologist experience. The diagnostic yield also compromised low abundance cells when morphology obscured inflammation reactive mesothelial cells. A reliable molecular marker that may complement diagnosis needed....